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Virginia/18 July 2008
From 2008.igem.org
(Difference between revisions)
(New page: ==Overnight== *plates A11() A12 and A13 *The screaning plasmid grew in overnight broth but we could not induce it to fluoresce *Overnight broths of BP2, BP3, PSB1A10, psb1A10 with arabinos...) |
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| - | ==Overnight== | + | ==Overnight Results== |
| - | *plates A11() A12 and A13 | + | *plates A11(PRET BP1) A12(B0032+BP2) and A13(B0032+BP3) all grew |
*The screaning plasmid grew in overnight broth but we could not induce it to fluoresce | *The screaning plasmid grew in overnight broth but we could not induce it to fluoresce | ||
*Overnight broths of BP2, BP3, PSB1A10, psb1A10 with arabinose, E0240 (3x) (needs promoter), A6(PRE BP1) with IPTG(lactose) | *Overnight broths of BP2, BP3, PSB1A10, psb1A10 with arabinose, E0240 (3x) (needs promoter), A6(PRE BP1) with IPTG(lactose) | ||
| + | |||
==Goals== | ==Goals== | ||
| - | *Miniprep the cells that grew in the broths | + | *Miniprep the cells that grew in the broths (BP2, BP3, PSB1A10, E0240_3x , A6) |
*SDS PAGE the PRE BP1 to see if the new pathway is being expressed | *SDS PAGE the PRE BP1 to see if the new pathway is being expressed | ||
*Overnight broth of RE-BP2 and RE-BP3 | *Overnight broth of RE-BP2 and RE-BP3 | ||
Latest revision as of 14:34, 18 July 2008
Overnight Results
- plates A11(PRET BP1) A12(B0032+BP2) and A13(B0032+BP3) all grew
- The screaning plasmid grew in overnight broth but we could not induce it to fluoresce
- Overnight broths of BP2, BP3, PSB1A10, psb1A10 with arabinose, E0240 (3x) (needs promoter), A6(PRE BP1) with IPTG(lactose)
Goals
- Miniprep the cells that grew in the broths (BP2, BP3, PSB1A10, E0240_3x , A6)
- SDS PAGE the PRE BP1 to see if the new pathway is being expressed
- Overnight broth of RE-BP2 and RE-BP3
- Digest and gel miniprepped DNA from today to verify what we have
- Try to express fluorescence by digesting and ligating PSB1a10 to
- remove cell death gene and grow in dh5alpha cells
- add a promoter between restriction sites to prove it can atleast fluoresce green
- Digest and ligate E0240 (3x) with the promoter R0040 to express green
