Wisconsin: Lignin Project/10 July 2008

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(Difference between revisions)

Latest revision as of 03:28, 29 October 2008

Team Sorbitol:

Got the sequencing data back and found that colony 2 was correct and will be used for all future experiments.
Started the following cultures:
DH5a-PBAD30 : to harvest the plasmid to clone out the srl operon
DH5a-pBAD30-srlD: from colony 2 to freeze down for back up
DH5a-pBAD18 - to insert srld and the operon into MG1655, JW3890, RL257
This was done to make chemically competent
Team Fungus:
Ran out gradient PCR products on 1% agarose gel. No bands.
Ran TAQ gradient PCR again in one more tube due to low amplification results using only 1 uL of cDNA template. Ran PCR out on gel. Only positive control showed up.
Made LB+Amp+XGal plates.
Attempted transformation again.

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-== Team Sorbitol ==
+<div style="background: #000; padding: 10px;">
+<div style="width:800px; height:147px; border:0px; margin:0px;">[[Image:Igemwibanner.gif]]</div>
+{|align="justify" style="color:#aada84;background-color:#000;" width="800 px"
+|'''Team Sorbitol:'''<br>
+Got the sequencing data back and found that colony 2 was correct and will be used for all future experiments.<br>
+Started the following cultures:<br>
+DH5a-PBAD30 : to harvest the plasmid to clone out the ''srl'' operon<br>
+DH5a-pBAD30-srlD: from colony 2 to freeze down for back up<br>
+DH5a-pBAD18 - to insert ''srld'' and the operon into MG1655, JW3890, RL257<br>
+This was done to make chemically competent<br>
+'''Team Fungus:'''<br>
+Ran out gradient PCR products on 1% agarose gel. No bands.<br>
+Ran TAQ gradient PCR again in one more tube due to low amplification results using only 1 uL of cDNA template.
+Ran PCR out on gel. Only positive control showed up.<br>
+Made LB+Amp+XGal plates.<br>
+Attempted transformation again.<br>
+|}