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Team:Hawaii/Notebook/2008-08- 9
From 2008.igem.org
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:* Plasmid prep of E0240, J32007, I14032 ('05 and '08 distributions), I51020 | :* Plasmid prep of E0240, J32007, I14032 ('05 and '08 distributions), I51020 | ||
| - | ::* Centrifuged for | + | ::* Centrifuged for 90 min. at 4C after adding KoAc |
===Cryostock=== | ===Cryostock=== | ||
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:* Redid RE digest overnight | :* Redid RE digest overnight | ||
| + | ===Made KoAc solution for plasmid preps=== | ||
| + | :<strong>Grace</strong> | ||
===Gel Purification=== | ===Gel Purification=== | ||
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:* Ran the large-scale PCR of aadA(BB), aadA(pRL1383a), and P1 lytic region on a gel, cut it out, and purified it using the Qiagen Kit | :* Ran the large-scale PCR of aadA(BB), aadA(pRL1383a), and P1 lytic region on a gel, cut it out, and purified it using the Qiagen Kit | ||
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= Discussion = | = Discussion = | ||
Latest revision as of 05:28, 13 August 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Plasmid prep
- Grace
- Plasmid prep of E0240, J32007, I14032 ('05 and '08 distributions), I51020
- Centrifuged for 90 min. at 4C after adding KoAc
Cryostock
- Grace
- Did not take out 2.4 ml of E. coli before doing plasmid prep (oops). Reinoculated for cryostocking tomorrow.
Construction of p+r+g, g+t, J33207+t
- Grace
- RE digest of nir+rbs, plac+rbs, GFP, B0015, J33207
- Digested nir+rbs, plac+rbs, J33207 with EcoRI and SpeI
- Digested GFP, B0015 with Pst1 and XbaI
- Ran digests on EtBr stained 2% agaorse gel
- Gel did not run despite using new TAE buffer
- Redid RE digest overnight
Made KoAc solution for plasmid preps
- Grace
Gel Purification
- Margaret
- Ran the large-scale PCR of aadA(BB), aadA(pRL1383a), and P1 lytic region on a gel, cut it out, and purified it using the Qiagen Kit
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
