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Team:Hawaii/Meeting/2008-05-29
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# Team Agreement (Gernot) | # Team Agreement (Gernot) | ||
# Presentations: | # Presentations: | ||
| - | ## | + | ## BioBricks "Other" (GK) |
| - | + | ||
## Plasmid (MR/NW) | ## Plasmid (MR/NW) | ||
## Plan Part B | ## Plan Part B | ||
| + | ### Margaret: cyan red dual plasmid system | ||
| + | ### Grace: lux operon night light | ||
| + | ### Krystle: bacterial export system, soluble proteins for crystallagraphy | ||
# Team Photo | # Team Photo | ||
# After 9-10am meeting, Jamie will show the lab safety, chemical storage, etc walkthrough. | # After 9-10am meeting, Jamie will show the lab safety, chemical storage, etc walkthrough. | ||
| Line 15: | Line 17: | ||
Present: GK, KS, MR, SC, GP, KS, NW | Present: GK, KS, MR, SC, GP, KS, NW | ||
| - | # | + | # Presentations: |
| + | ## [[Image:Biobrick_parts_other.pdf | BioBricks Other]] (GK) | ||
| + | ### Other Parts (GK): Construction Intermediates, PCR Primers, Tags & Modifiers, Other Other Parts, Deleted Parts, Bacteriophate T7 Phage Parts & RNA Stabilizers. | ||
| + | ## Plasmid (MR/NW) | ||
| + | ### RSF1010 Overview: OriT, tra, mob overview | ||
| + | ### Proposed design, 4 BioBrick subcomponents | ||
| + | ### RNA Stability questions (regions past coding seq, how far do we PCR past carboxy terminus for possible RNA stability features? Are there any?) | ||
| + | ### Discussion regarding its usefulness (will be used by a lot of people) | ||
| + | ## Plan Part B | ||
| + | ### Margaret: cyan red dual plasmid system | ||
| + | ### Grace: lux operon night light | ||
| + | ### Krystle: bacterial export system, soluble proteins for crystallagraphy | ||
| + | # Team Photo | ||
| + | # Jamie showed us around the lab safety, talked about chemical storage, etc walkthrough. | ||
== Action Items == | == Action Items == | ||
| - | * | + | * MR write up formal proposal with details on synthetic plasmid |
| - | * | + | * ''E. coli'' grown and frozen, possible competent cell made |
| + | * get autoclave trained | ||
== Coming Up == | == Coming Up == | ||
Latest revision as of 07:26, 30 May 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Contents |
Agenda
- Team Agreement (Gernot)
- Presentations:
- BioBricks "Other" (GK)
- Plasmid (MR/NW)
- Plan Part B
- Margaret: cyan red dual plasmid system
- Grace: lux operon night light
- Krystle: bacterial export system, soluble proteins for crystallagraphy
- Team Photo
- After 9-10am meeting, Jamie will show the lab safety, chemical storage, etc walkthrough.
Minutes
Present: GK, KS, MR, SC, GP, KS, NW
- Presentations:
- File:Biobrick parts other.pdf (GK)
- Other Parts (GK): Construction Intermediates, PCR Primers, Tags & Modifiers, Other Other Parts, Deleted Parts, Bacteriophate T7 Phage Parts & RNA Stabilizers.
- Plasmid (MR/NW)
- RSF1010 Overview: OriT, tra, mob overview
- Proposed design, 4 BioBrick subcomponents
- RNA Stability questions (regions past coding seq, how far do we PCR past carboxy terminus for possible RNA stability features? Are there any?)
- Discussion regarding its usefulness (will be used by a lot of people)
- Plan Part B
- Margaret: cyan red dual plasmid system
- Grace: lux operon night light
- Krystle: bacterial export system, soluble proteins for crystallagraphy
- File:Biobrick parts other.pdf (GK)
- Team Photo
- Jamie showed us around the lab safety, talked about chemical storage, etc walkthrough.
Action Items
- MR write up formal proposal with details on synthetic plasmid
- E. coli grown and frozen, possible competent cell made
- get autoclave trained
Coming Up
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
