Team:Hawaii/Meeting/2008-05-29

From 2008.igem.org

(Difference between revisions)
 
(2 intermediate revisions not shown)
Line 4: Line 4:
# Team Agreement (Gernot)
# Team Agreement (Gernot)
# Presentations:
# Presentations:
-
## [[Image:Biobrick_parts_other.pdf | BioBricks Other]] (GK)
+
## BioBricks "Other" (GK)
-
### Other Parts (GK): Construction Intermediates, PCR Primers, Tags & Modifiers, Other Other Parts, Deleted Parts, Bacteriophate T7 Phage Parts & RNA Stabilizers.
+
## Plasmid (MR/NW)
## Plasmid (MR/NW)
-
### RSF1010 Overview: OriT, tra, mob overview
 
-
### Proposed design, 4 BioBrick subcomponents
 
-
### RNA Stability questions (regions past coding seq, how far do we PCR past carboxy terminus for possible RNA stability features? Are there any?)
 
-
### Discussion regarding its usefulness (will be used by a lot of people)
 
## Plan Part B  
## Plan Part B  
### Margaret: cyan red dual plasmid system
### Margaret: cyan red dual plasmid system
### Grace: lux operon night light
### Grace: lux operon night light
-
#### rubisco promoter may be toxic, could use KaiABC gene from Harvard 07? team.
 
-
#### already have degredation promoter
 
### Krystle: bacterial export system, soluble proteins for crystallagraphy
### Krystle: bacterial export system, soluble proteins for crystallagraphy
# Team Photo
# Team Photo
Line 24: Line 17:
Present: GK, KS, MR, SC, GP, KS, NW
Present: GK, KS, MR, SC, GP, KS, NW
-
#  
+
# Presentations:
 +
## [[Image:Biobrick_parts_other.pdf | BioBricks Other]] (GK)
 +
### Other Parts (GK): Construction Intermediates, PCR Primers, Tags & Modifiers, Other Other Parts, Deleted Parts, Bacteriophate T7 Phage Parts & RNA Stabilizers.
 +
## Plasmid (MR/NW)
 +
### RSF1010 Overview: OriT, tra, mob overview
 +
### Proposed design, 4 BioBrick subcomponents
 +
### RNA Stability questions (regions past coding seq, how far do we PCR past carboxy terminus for possible RNA stability features? Are there any?)
 +
### Discussion regarding its usefulness (will be used by a lot of people)
 +
## Plan Part B
 +
### Margaret: cyan red dual plasmid system
 +
### Grace: lux operon night light
 +
### Krystle: bacterial export system, soluble proteins for crystallagraphy
 +
# Team Photo
 +
# Jamie showed us around the lab safety, talked about chemical storage, etc walkthrough.
== Action Items ==
== Action Items ==
* MR write up formal proposal with details on synthetic plasmid
* MR write up formal proposal with details on synthetic plasmid
-
*  
+
* ''E. coli'' grown and frozen, possible competent cell made
 +
* get autoclave trained
== Coming Up ==
== Coming Up ==

Latest revision as of 07:26, 30 May 2008

Projects Events Resources
Sponsors Experiments Milestones Protocols
Notebook (t) Meetings (t)

Contents

Agenda

  1. Team Agreement (Gernot)
  2. Presentations:
    1. BioBricks "Other" (GK)
    2. Plasmid (MR/NW)
    3. Plan Part B
      1. Margaret: cyan red dual plasmid system
      2. Grace: lux operon night light
      3. Krystle: bacterial export system, soluble proteins for crystallagraphy
  3. Team Photo
  4. After 9-10am meeting, Jamie will show the lab safety, chemical storage, etc walkthrough.

Minutes

Present: GK, KS, MR, SC, GP, KS, NW

  1. Presentations:
    1. File:Biobrick parts other.pdf (GK)
      1. Other Parts (GK): Construction Intermediates, PCR Primers, Tags & Modifiers, Other Other Parts, Deleted Parts, Bacteriophate T7 Phage Parts & RNA Stabilizers.
    2. Plasmid (MR/NW)
      1. RSF1010 Overview: OriT, tra, mob overview
      2. Proposed design, 4 BioBrick subcomponents
      3. RNA Stability questions (regions past coding seq, how far do we PCR past carboxy terminus for possible RNA stability features? Are there any?)
      4. Discussion regarding its usefulness (will be used by a lot of people)
    3. Plan Part B
      1. Margaret: cyan red dual plasmid system
      2. Grace: lux operon night light
      3. Krystle: bacterial export system, soluble proteins for crystallagraphy
  2. Team Photo
  3. Jamie showed us around the lab safety, talked about chemical storage, etc walkthrough.

Action Items

  • MR write up formal proposal with details on synthetic plasmid
  • E. coli grown and frozen, possible competent cell made
  • get autoclave trained

Coming Up


[http://manoa.hawaii.edu/ Sponsor_UHM.gif][http://manoa.hawaii.edu/ovcrge/ Sponsor_OVCRGE.gif][http://www.ctahr.hawaii.edu Sponsor_CTAHR.gif]

Retrieved from "http://2008.igem.org/Team:Hawaii/Meeting/2008-05-29"