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Alberta NINT/12 August 2008
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[[Team:Alberta_NINT/Notebook | < Back to notebook]] | [[Team:Alberta_NINT/Notebook | < Back to notebook]] | ||
| - | [[ | + | [[Alberta_NINT/11_August_2008 | < Previous entry]] | [[Alberta_NINT/13_August_2008 | Next entry >]] |
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lab work (JD): | lab work (JD): | ||
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Incubated at 37 overnight | Incubated at 37 overnight | ||
| - | + | lab work (SD): | |
| + | Transformation results: | ||
| + | K102031 (negative control) 1 | ||
| + | TA2 7 | ||
| + | TA3 4 | ||
| + | TA6 1 | ||
| + | TA7 2 | ||
| + | Made overnights of the colonies on these plates. | ||
| + | Punched out pSB1A7 and pSB1A3 from the parts registry book and transformed them into XL1-B cells. | ||
Latest revision as of 23:07, 11 October 2008
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lab work (JD):
Gel purification of TAIn_2, 3, 6, 7/X+H
Collected Quantitative Data
Performed Ligations of K102011+TAIn_2,3,6,7
Transformed XL1-B cells with ligations
Plated on LB+kan35
Incubated at 37 overnight
lab work (SD):
Transformation results:
K102031 (negative control) 1
TA2 7
TA3 4
TA6 1
TA7 2
Made overnights of the colonies on these plates.
Punched out pSB1A7 and pSB1A3 from the parts registry book and transformed them into XL1-B cells.
