Alberta NINT/27 May 2008

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lab work (SD): DNA digest of K102000 with SpeI + PstI.  Incubated at 37 C for 1 hour.  Run on 2% agarose gel.  ~3 kbp fragment expected.  Ligated K102000 with E0433 and K102000 with null_output.
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[[Team:Alberta_NINT/Notebook | < Back to notebook]]
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lab work (JD): Recovered Part BBA_R011 from iGEM package, transformed XL1-B cells and plated on LB+Amp100 plates.  Created 2 O/Ns as well.
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[[Alberta_NINT/23_May_2008 | < Previous entry]] | [[Alberta_NINT/28_May_2008 | Next entry >]]
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lab work (SD):  
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 +
            DNA digest of K102000 with SpeI + PstI.  Incubated at 37 C for 1 hour.   
 +
            Run on 2% agarose gel.  ~3 kbp fragment expected.   
 +
            Ligated K102000 with E0433 and K102000 with null_output.
 +
 
 +
lab work (JD):  
 +
 
 +
            Recovered Part BBA_R011 from iGEM package  
 +
            Transformed XL1-B cells and plated on LB+Amp100 plates.   
 +
            Created 2 O/Ns as well.
 +
 
 +
lab work (WM):
 +
 
 +
            Prepare BBa_K102010 fragments for ligation.
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            K102010 is AraC::PromBad::TA0In
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            AraC::PromBad comes is in BBa_I0500/SpeI+PstI (5.6kb fragment, including plasmid)
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            TA0In (200bp) was synthesized commercially and placed in a vector.
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            Gel-purify BBa_I0500/SpeI+PstI and TA0In?XbaI+PstI fragments from 2% agarose gel.
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  [[Image:NINT_WMp22.JPG | 800 px]]

Latest revision as of 21:30, 11 October 2008

< Back to notebook

< Previous entry | Next entry >

lab work (SD): 

           DNA digest of K102000 with SpeI + PstI.  Incubated at 37 C for 1 hour.  
           Run on 2% agarose gel.  ~3 kbp fragment expected.  
           Ligated K102000 with E0433 and K102000 with null_output.

lab work (JD): 

            Recovered Part BBA_R011 from iGEM package 
            Transformed XL1-B cells and plated on LB+Amp100 plates.  
            Created 2 O/Ns as well.

lab work (WM): 

            Prepare BBa_K102010 fragments for ligation.
            K102010 is AraC::PromBad::TA0In
            AraC::PromBad comes is in BBa_I0500/SpeI+PstI (5.6kb fragment, including plasmid)
            TA0In (200bp) was synthesized commercially and placed in a vector.
            Gel-purify BBa_I0500/SpeI+PstI and TA0In?XbaI+PstI fragments from 2% agarose gel.
 NINT WMp22.JPG
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