Team:Hawaii

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<span class=”plainlinks”>[https://2008.igem.org/Team:Hawaii/Project https://static.igem.org/mediawiki/2008/5/5b/Oahu_makapuu_UHM_TKT.jpg]</span>
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{{Team:Hawaii/Header}}
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[[Image:cyanoblob.jpg|left|130px|]]
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==The Project==
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:Cyanobacteria are photosynthetic bacteria that can convert carbon dioxide to sugars.  The main goal of the Hawaii team is to make a "toolkit" for cyanobacteria, which hold promise as producers of biofuels and other bioproducts. 
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:We constructed a number of tools to more easily and effectively engineer cyanobacteria, including
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::::1) a BioBrick switch that turns on a downstream gene when nitrate is added,
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::::2) a BioBrick that mediates protein export from the cell into the surrounding medium and
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::::3) a naturally occurring shuttle vector to BioBrick format to allow easy transfer of existing and new BioBricks into cyanobacteria.
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:Our toolkit was designed to allow controlled production and recovery of bioproducts. Additionally, our vector has the potential to transform other gram negative bacteria and possibly even plants via ''Agrobacterium''.
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|style="background-color:WhiteSmoke;border-width: 0px;padding: 3px;text-align:left;"|Our team aims to create the framework and tools for expanding the scope of BioBrick synthetic biology to organisms other than E. coli.  Creation of a mobilizable broad-host-range plasmid enables the transfer of modularized and abstracted genetic components to act in wide range of organisms that share a similar pool of intermediate metabolites.  A broad-host-range mobilizable vector would serve as a channel to "open source" the rich diversity of genetic information between organisms in a laboratory setting.  We envision a wide diversity of future broad-host-range BioBrick parts, created and transported, with this or similar BioBrick vectors; each designated to be compatible with specific organism(s). These modules can also be combinatorially transformed or combined into organisms as larger devices to produce desired products. [[Team:Hawaii/Project|more...]]
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!<big>Resources</big>
 
!<big>Parts</big>
!<big>Parts</big>
!<big>Help</big>
!<big>Help</big>
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!<big>Links</big>
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!<big>Links/Resources</big>
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*[http://packrat.stjohn.hawaii.edu/prestinglab/wiki/iGEM:2008/References References]
 
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*[https://2008.igem.org/Team:Hawaii/Protocols Protocols]
 
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*[http://packrat.stjohn.hawaii.edu/prestinglab/wiki/Inventory Inventory]
 
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*[http://packrat.stjohn.hawaii.edu/prestinglab/wiki/Shopping_List Ordering]
 
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*[http://partsregistry.org/cgi/partsdb/search.cgi Parts Search]
*[http://partsregistry.org/cgi/partsdb/search.cgi Parts Search]
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*[[Team:Hawaii/Wiki Help|Wiki Help]]
*[[Team:Hawaii/Wiki Help|Wiki Help]]
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*[http://packrat.stjohn.hawaii.edu/prestinglab/wiki/iGEM:2008 Hawaii Team Private Homepage]
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*[https://2008.igem.org/Team:Hawaii/Protocols Protocols]
*[https://2008.igem.org iGEM 2008 Homepage]
*[https://2008.igem.org iGEM 2008 Homepage]
*[http://partsregistry.org Parts Registry Homepage]
*[http://partsregistry.org Parts Registry Homepage]
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Search <input type="radio" name="sitesearch" value="2008.igem.org"/>iGEM 2008<input type="radio" name="sitesearch" value="http://parts.mit.edu/igem07/"/>iGEM 2007<input type="radio" name="sitesearch" value="en.wikipedia.org"/>Wikipedia
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Search <input type="radio" name="sitesearch" value="2008.igem.org"/>iGEM 2008<input type="radio" name="sitesearch" value="https://2007.igem.org/"/>iGEM 2007<input type="radio" name="sitesearch" value="en.wikipedia.org"/>Wikipedia
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{{Team:Hawaii/Footer}}
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Latest revision as of 03:55, 30 October 2008

Oahu_makapuu_UHM_TKT.jpg

Projects Events Resources
Sponsors Experiments Milestones Protocols
Notebook (t) Meetings (t)
Cyanoblob.jpg

The Project

Cyanobacteria are photosynthetic bacteria that can convert carbon dioxide to sugars. The main goal of the Hawaii team is to make a "toolkit" for cyanobacteria, which hold promise as producers of biofuels and other bioproducts.
We constructed a number of tools to more easily and effectively engineer cyanobacteria, including
1) a BioBrick switch that turns on a downstream gene when nitrate is added,
2) a BioBrick that mediates protein export from the cell into the surrounding medium and
3) a naturally occurring shuttle vector to BioBrick format to allow easy transfer of existing and new BioBricks into cyanobacteria.
Our toolkit was designed to allow controlled production and recovery of bioproducts. Additionally, our vector has the potential to transform other gram negative bacteria and possibly even plants via Agrobacterium.



Parts Help Links/Resources
  • [http://partsregistry.org/cgi/partsdb/search.cgi Parts Search]
  • [http://partsregistry.org/assembly/libraries.cgi?id=15 Parts Kit Content (Spring 2008)]
  • [http://partsregistry.org/Help:IGEM_08_DNA_distribution Parts Kit Instructions]
  • Parts We Submitted
  • [http://partsregistry.org/wiki/index.php/Help:Contents BioBrick Help]
  • Wiki Help

Search iGEM 2008iGEM 2007Wikipedia

[http://manoa.hawaii.edu/ Sponsor_UHM.gif][http://manoa.hawaii.edu/ovcrge/ Sponsor_OVCRGE.gif][http://www.ctahr.hawaii.edu Sponsor_CTAHR.gif]


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