Team:KULeuven/Primers

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Latest revision as of 00:43, 27 October 2008

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Table of Oligos
Number	Name	Sequence	Length	Tm (°C)	Comments
1076	T7 Fw, no tag	ATGAATTCGC GGCCGCTTCT AGATGAACAC GATTAACATC GC	42bp	66.7	Forward T7 RNApol primer, without UmuD tag
1077	T7 Rev	CTGCAGCGGC CGCTACTAGT ATTATTACGC GAACGCGAAG TCCG	44bp	59.9	Reverse T7 RNApol primer
1078	T7 Fw, UmuD tag, part 1	CCGCTATTTA GCGATCTTGT TCAGTGTGGC TTTCCTTCAC CGATGAACAC GATTAACATC GC	62bp	51.0	Forward T7 RNApol primer, with UmuD tag, part 1
1079	T7 Fw, UmuD tag, part 2	ATGAATTCGC GGCCGCTTCT AGATGTTGTT TATCAAGCCT GCGGATCTCC GCGAAATTGT GACTTTTCCG CTATTTAGCG ATCTTGTTCA G	91bp	55.8	Forward T7 RNApol primer, with UmuD tag, part 2, very expensive
1084	GFP-LVA Fw	CATCATGAAT TCGCGGCCGC TTCTAGATGC GTAAAGGAGA AGAACTTTTC ACTGG	55bp	58.9	Forward GFP-LVA primer
1085	GFP-LVA Rev	CTGCAGCGGC CGCTACTAGT ATTATTAAGC TACTAAAGCG TAGTTTTCGT CGTTTGCAGC AGGC	64bp	64.9	Reverse GFP-LVA primer
1074	VF2	TGCCACCTGA CGTCTAAGAA	20bp	55.9	Forward primer for sequence analysis
1075	VR	ATTACCGCCT TTGAGTGAGC	20bp	55.3	Reverse primer for sequence analysis
1100	antisense luxI Fw	CATCAGGAAT TCGCGGCCGC TTCTAGTTAT TAAGCTACTA AAGCGTAGTT TTC	53bp	51.6	Forward primer for construction of antisense luxI, contains prefix, contains additional CATCAG
1101	antisense luxI Rev	CTGCAGCGGC CGCTACTAGT AATGACTATA ATGATAAAAA AATCGG	46bp	47.8	Reverse primer for construction of antisense luxI, contains suffix, 25 bp annealing
1127	ccdB coding Fw	CATCATGAAT TCGCGGCCGC TTCTAGATGC AGTTTAAGGT TTACACC	47	50.9	Forward primer for amplification of ccdB coding sequence, contains prefix, additional CATCAT
1128	ccdB coding Rev	CTGCAGCGGC CGCTACTAGT ATTATATTCC CCAGAACATC AGG	43	51.8	Reverse primer for amplification of ccdB, contains suffix
1129	Hybrid promoter left linker	CATCATGAAT TCGCGGCCGC TTCTAGACCT GTAGGATCGT ACAGGTTTAC TAAAGATTCC TTTAG	65	check	Left linker for construction of hybrid promoter by end filling, repressed by P22 c2 (output of memory in thr OFF state) and activated by HSL_LuxR, 16 bp overlap, contains prefix, additional CATCAT
1130	Hybrid promoter right linker	TACTAGTAGC GGCCGCTGCA GGAAATTAAA GAACACTTAA ATTATAAACT AAAGGAATCT TTAG	64	check	right linker for construction of hybrid promoter by end filling, repressed by P22 c2 (output of memory in thr OFF state) and activated by HSL_LuxR, 16 bp overlap, contains suffix
1131	BBa_I714070 lock Fw	CATCATGAAT TCGCGGCCGC TTCTAGAGTG TAGACCGAAC TAGAATCACC TC	52	58.7	Forward primer for amplication of lock from BBa_I714070 (J23078), contains scar, contains prefix, additional CATCAT
1132	BBa_I714070 lock Rev	CTGCAGCGGC CGCTACTAGT ATC	23	61.5	Reverse primer for amplication of lock from BBa_I714070 (J23078), contains suffix
1133	BBa_I714074 key Fw	CATCATGAAT TCGCGGCCGC TTCTAGAGAC CCAAAAGCAA GAGGTG	46	57.3	Forward primer for amplication of key from part BBa_I714074 - J23066_B0015 (J23008, J23009, B0010, B0012), contains prefix, additional CATCAT
1134	BBa_I714074 key Rev	CTGCAGCGGC CGCTACTAGT ATATAAAC	28	60.3	Reverse primer for amplication of key from part BBa_I714074 - J23066_B0015 (J23008, J23009, B0010, B0012), contains suffix
1152	CATCAT -Prefix	CATCATGAAT TCGCGGCCGC TTC	23	61.2	Fwd primer to anneal to the Prefix. Orginally made for the PCR after end-filling.
1153	Suffix	CTGCAGCGGC CGCTACTAG	19	60.9	Rev primer to anneal to the Suffix. Orginally made for the PCR after end-filling.
1156	T7 Fw, UmuD tag, part 2.1	CAAGCCTGCG GATCTCCGCG AAATTGTGAC TTTTCCGCTA TTTAGCGATC TTGTTCAG	58	55.9	Forward T7 RNApol primer, with UmuD tag, part 2.1
1157	T7 Fw, UmuD tag, part 2.2	CATCATGAAT TCGCGGCCGC TTCTAGATGT TGTTTATCAA GCCTGCGGAT CTCC	54	56.0	Forward T7 RNApol primer, with UmuD tag, part 2.2

@@ Line 1: / Line 1: @@
+{{:Team:KULeuven/Tools/Styling}}
+{{:Team:KULeuven/Tools/Scripting}}
 {{:Team:KULeuven/Tools/Header}}
@@ Line 9: / Line 11: @@
 </html>
-{|cellspacing="1" width=100%
-! style="background:#003E81; color: white" colspan="6" | Table of Oligos
+{|cellspacing="1"; width="85%"
+! style="background:#003E81; color: white" colspan="6" align="center" height="30px"| '''Table of Oligos'''
 |-style="background:#99CCFF" align="center"
-| width=5% | Number
+| width=5% | '''Number'''
-| width=10% | Name
+| width=10% | '''Name'''
-| width=55% | Sequence
+| width=35% | '''Sequence'''
-| width=5% | Length
+| width=5% | '''Length'''
-| width=5% | Tm (°C)
+| width=5% | '''Tm (°C)'''
-| width=20% | Comments
+| width=25% | '''Comments'''
 {{:Team:KULeuven/Tools/Oligo
 |nr=1076
@@ Line 44: / Line 49: @@
 {{:Team:KULeuven/Tools/Oligo
 |nr=1079
-|name=T7 Tw, UmuD tag, part 2
+|name=T7 Fw, UmuD tag, part 2
 |sequence= <div class="code">ATGAATTCGC GGCCGCTTCT AGATGTTGTT TATCAAGCCT GCGGATCTCC GCGAAATTGT GACTTTTCCG CTATTTAGCG ATCTTGTTCA G</div>
 |length=91bp
@@ Line 134: / Line 139: @@
 |name='''BBa_I714070''' lock Fw
 |sequence=<div class="code">CATCATGAAT TCGCGGCCGC TTCTAGAGTG TAGACCGAAC TAGAATCACC TC</div>
-|Tm='''check'''
+|Tm=58.7
 |length=52
 |comments=Forward primer for amplication of lock from '''BBa_I714070''' (J23078), contains scar, contains prefix, additional <div class="code">CATCAT</div>
@@ Line 161: / Line 166: @@
 |length=28
 |comments=Reverse primer for amplication of key from part '''BBa_I714074''' - J23066_B0015 (J23008, J23009, B0010, B0012), contains suffix
+}}
+{{:Team:KULeuven/Tools/Oligo
+|nr=1152
+|name=<div class="code">CATCAT</div>-Prefix
+|sequence=<div class="code">CATCATGAAT TCGCGGCCGC TTC</div>
+|Tm=61.2
+|length=23
+|comments=Fwd primer to anneal to the Prefix. Orginally made for the PCR after end-filling.
+}}
+{{:Team:KULeuven/Tools/Oligo
+|nr=1153
+|name=Suffix
+|sequence=<div class="code">CTGCAGCGGC CGCTACTAG</div>
+|Tm=60.9
+|length=19
+|comments=Rev primer to anneal to the Suffix. Orginally made for the PCR after end-filling.
+}}
+{{:Team:KULeuven/Tools/Oligo
+|nr=1156
+|name=T7 Fw, UmuD tag, part 2.1
+|sequence=<div class="code">CAAGCCTGCG GATCTCCGCG AAATTGTGAC TTTTCCGCTA TTTAGCGATC TTGTTCAG</div>
+|Tm=55.9
+|length=58
+|comments=Forward T7 RNApol primer, with UmuD tag, part 2.1
+}}
+{{:Team:KULeuven/Tools/Oligo
+|nr=1157
+|name=T7 Fw, UmuD tag, part 2.2
+|sequence=<div class="code">CATCATGAAT TCGCGGCCGC TTCTAGATGT TGTTTATCAA GCCTGCGGAT CTCC</div>
+|Tm=56.0
+|length=54
+|comments=Forward T7 RNApol primer, with UmuD tag, part 2.2
 }}
 |}