Team:Valencia/Notebook/Protocols

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{{Valencia/Menu}}
<div class="valenciaMain">
<div class="valenciaMain">
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[[Team:Valencia|<font color="#047DB5">Home</font>]]
 
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&nbsp;&nbsp;|&nbsp;&nbsp;[[Team:Valencia/Team|<font color="#047DB5">The Team</font>]]
 
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&nbsp;&nbsp;|&nbsp;&nbsp;[[Team:Valencia/Project|<font color="#047DB5">The Project</font>]]
 
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&nbsp;&nbsp;|&nbsp;&nbsp;[[Team:Valencia/Parts|<font color="#047DB5">Parts Submitted to the Registry</font>]]
 
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&nbsp;&nbsp;|&nbsp;&nbsp;[[Team:Valencia/Modeling|<font color="#047DB5">Modeling</font>]]
 
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&nbsp;&nbsp;|&nbsp;&nbsp;[[Team:Valencia/Notebook|<font color="#047DB5">Notebook</font>]]
 
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|1ml <br>
|1ml <br>
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|-
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|Half culture plate <br>
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|Half volume of a culture plate <br>
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== SD Medium ==
== SD Medium ==
{|
{|
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|Yeast Nitrogen Base
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|Yeast Nitrogen Base &nbsp;
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||0.67%
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|0.67%
|-
|-
|Glucose
|Glucose
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== SP liquid medium ==
== SP liquid medium ==
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Yeast Nitrogen Base 0.67% <br>
+
{|
-
Potassium phosohate 0.1% <br>
+
|Yeast Nitrogen Base
-
Ammonium sulfate    0.12% <br>
+
|0.67%
-
Glucose            0.05% <br>
+
|-
-
Lactic acid        2% <br>
+
|Potassium phosohate &nbsp;
-
Casamino acids      0.1% <br>
+
|0.1%
-
Tryptophan          20 mg/l <br>
+
|-
-
Adenine            40 mg/l <br>
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|Ammonium sulfate     
-
 
+
|0.12%  
 +
|-
 +
|Glucose             
 +
|0.05%  
 +
|-
 +
|Lactic acid         
 +
|2%  
 +
|-
 +
|Casamino acids       
 +
|0.1%  
 +
|-
 +
|Tryptophan           
 +
|20 mg/l
 +
|-
 +
|Adenine             
 +
|40 mg/l  
 +
|}
== YPKAc Medium ==
== YPKAc Medium ==
-
Yeast extract      1% <br>
+
{|
-
Peptone            2% <br>
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|Yeast extract       
-
Potassium acetate   2% <br>
+
|1%  
-
Tryptophan          20 mg/l <br>
+
|-
-
Adenine            40 mg/l <br>
+
|Peptone             
 +
|2%  
 +
|-
 +
|Potassium acetate &nbsp; 
 +
|2%  
 +
|-
 +
|Tryptophan           
 +
|20 mg/l  
 +
|-
 +
|Adenine             
 +
|40 mg/l  
 +
|}
</div>
</div>

Latest revision as of 12:55, 5 October 2008


Glycerol Stock

2 ml Eppendorf containing:

Sterile Glycerol (stock 50 %) 1ml
Half volume of a culture plate


SD Medium

Yeast Nitrogen Base   0.67%
Glucose 2%
Agar 2%
Casamino acids 0.1%
Tryptophan 20 mg/l
Adenine 40 mg/l

SP liquid medium

Yeast Nitrogen Base 0.67%
Potassium phosohate   0.1%
Ammonium sulfate 0.12%
Glucose 0.05%
Lactic acid 2%
Casamino acids 0.1%
Tryptophan 20 mg/l
Adenine 40 mg/l

YPKAc Medium

Yeast extract 1%
Peptone 2%
Potassium acetate   2%
Tryptophan 20 mg/l
Adenine 40 mg/l


Retrieved from "http://2008.igem.org/Team:Valencia/Notebook/Protocols"