Edinburgh/13 August 2008

From 2008.igem.org

(Difference between revisions)
(New page: <html> <head> <title>Edinburgh iGEM 2008</title> <script type="text/javascript"> //Drop Down Tabs Menu- Author: Dynamic Drive (http://www.dynamicdrive.com) //Created: May 16th, 07' var ta...)
 
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<html>
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<div id="header">{{Template:Team:Edinburgh/Templates/notebook-entry/header}}</div>
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<head>
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<title>Edinburgh iGEM 2008</title>
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<script type="text/javascript">
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//Drop Down Tabs Menu- Author: Dynamic Drive (http://www.dynamicdrive.com)
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//Created: May 16th, 07'
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var tabdropdown={
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:::: '''[[Edinburgh/12_August_2008|< Previous Entry]]'''
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disappeardelay: 200, //set delay in miliseconds before menu disappears onmouseout
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disablemenuclick: false, //when user clicks on a menu item with a drop down menu, disable menu item's link?
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enableiframeshim: 1, //1 or 0, for true or false
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//No need to edit beyond here////////////////////////
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dropmenuobj: null, ie: document.all, firefox: document.getElementById&&!document.all, previousmenuitem:null,
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currentpageurl: window.location.href.replace("http://"+window.location.hostname, "").replace(/^\//, ""), //get current page url (minus hostname, ie: http://www.dynamicdrive.com/)
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getposOffset:function(what, offsettype){
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var totaloffset=(offsettype=="left")? what.offsetLeft : what.offsetTop;
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var parentEl=what.offsetParent;
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while (parentEl!=null){
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totaloffset=(offsettype=="left")? totaloffset+parentEl.offsetLeft : totaloffset+parentEl.offsetTop;
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parentEl=parentEl.offsetParent;
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}
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return totaloffset;
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},
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showhide:function(obj, e, obj2){ //obj refers to drop down menu, obj2 refers to tab menu item mouse is currently over
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if (this.ie || this.firefox)
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this.dropmenuobj.style.left=this.dropmenuobj.style.top="-500px"
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if (e.type=="click" && obj.visibility==hidden || e.type=="mouseover"){
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if (obj2.parentNode.className.indexOf("default")==-1) //if tab isn't a default selected one
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obj2.parentNode.className="selected"
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obj.visibility="visible"
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}
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else if (e.type=="click")
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obj.visibility="hidden"
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},
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iecompattest:function(){
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return (document.compatMode && document.compatMode!="BackCompat")? document.documentElement : document.body
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},
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clearbrowseredge:function(obj, whichedge){
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var edgeoffset=0
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if (whichedge=="rightedge"){
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var windowedge=this.ie && !window.opera? this.standardbody.scrollLeft+this.standardbody.clientWidth-15 : window.pageXOffset+window.innerWidth-15
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this.dropmenuobj.contentmeasure=this.dropmenuobj.offsetWidth
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if (windowedge-this.dropmenuobj.x < this.dropmenuobj.contentmeasure)  //move menu to the left?
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edgeoffset=this.dropmenuobj.contentmeasure-obj.offsetWidth
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}
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else{
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var topedge=this.ie && !window.opera? this.standardbody.scrollTop : window.pageYOffset
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var windowedge=this.ie && !window.opera? this.standardbody.scrollTop+this.standardbody.clientHeight-15 : window.pageYOffset+window.innerHeight-18
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this.dropmenuobj.contentmeasure=this.dropmenuobj.offsetHeight
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if (windowedge-this.dropmenuobj.y < this.dropmenuobj.contentmeasure){ //move up?
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edgeoffset=this.dropmenuobj.contentmeasure+obj.offsetHeight
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if ((this.dropmenuobj.y-topedge)<this.dropmenuobj.contentmeasure) //up no good either?
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edgeoffset=this.dropmenuobj.y+obj.offsetHeight-topedge
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}
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this.dropmenuobj.firstlink.style.borderTopWidth=(edgeoffset==0)? 0 : "1px" //Add 1px top border to menu if dropping up
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}
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return edgeoffset
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},
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dropit:function(obj, e, dropmenuID){
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if (this.dropmenuobj!=null){ //hide previous menu
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this.dropmenuobj.style.visibility="hidden" //hide menu
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if (this.previousmenuitem!=null && this.previousmenuitem!=obj){
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if (this.previousmenuitem.parentNode.className.indexOf("default")==-1) //If the tab isn't a default selected one
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this.previousmenuitem.parentNode.className=""
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}
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}
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this.clearhidemenu()
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if (this.ie||this.firefox){
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obj.onmouseout=function(){tabdropdown.delayhidemenu(obj)}
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obj.onclick=function(){return !tabdropdown.disablemenuclick} //disable main menu item link onclick?
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this.dropmenuobj=document.getElementById(dropmenuID)
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this.dropmenuobj.onmouseover=function(){tabdropdown.clearhidemenu()}
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this.dropmenuobj.onmouseout=function(e){tabdropdown.dynamichide(e, obj)}
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this.dropmenuobj.onclick=function(){tabdropdown.delayhidemenu(obj)}
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this.showhide(this.dropmenuobj.style, e, obj)
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this.dropmenuobj.x=this.getposOffset(obj, "left")
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this.dropmenuobj.y=this.getposOffset(obj, "top")
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this.dropmenuobj.style.left=this.dropmenuobj.x-this.clearbrowseredge(obj, "rightedge")+"px"
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this.dropmenuobj.style.top=this.dropmenuobj.y-this.clearbrowseredge(obj, "bottomedge")+obj.offsetHeight+1+"px"
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this.previousmenuitem=obj //remember main menu item mouse moved out from (and into current menu item)
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this.positionshim() //call iframe shim function
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}
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},
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contains_firefox:function(a, b) {
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while (b.parentNode)
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if ((b = b.parentNode) == a)
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return true;
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return false;
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},
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dynamichide:function(e, obj2){ //obj2 refers to tab menu item mouse is currently over
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var evtobj=window.event? window.event : e
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if (this.ie&&!this.dropmenuobj.contains(evtobj.toElement))
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this.delayhidemenu(obj2)
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else if (this.firefox&&e.currentTarget!= evtobj.relatedTarget&& !this.contains_firefox(evtobj.currentTarget, evtobj.relatedTarget))
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this.delayhidemenu(obj2)
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},
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delayhidemenu:function(obj2){
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this.delayhide=setTimeout(function(){tabdropdown.dropmenuobj.style.visibility='hidden'; if (obj2.parentNode.className.indexOf('default')==-1) obj2.parentNode.className=''},this.disappeardelay) //hide menu
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},
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clearhidemenu:function(){
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if (this.delayhide!="undefined")
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clearTimeout(this.delayhide)
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},
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+
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positionshim:function(){ //display iframe shim function
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if (this.enableiframeshim && typeof this.shimobject!="undefined"){
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-
if (this.dropmenuobj.style.visibility=="visible"){
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-
this.shimobject.style.width=this.dropmenuobj.offsetWidth+"px"
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-
this.shimobject.style.height=this.dropmenuobj.offsetHeight+"px"
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-
this.shimobject.style.left=this.dropmenuobj.style.left
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this.shimobject.style.top=this.dropmenuobj.style.top
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-
}
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this.shimobject.style.display=(this.dropmenuobj.style.visibility=="visible")? "block" : "none"
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-
}
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},
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hideshim:function(){
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if (this.enableiframeshim && typeof this.shimobject!="undefined")
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this.shimobject.style.display='none'
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},
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isSelected:function(menuurl){
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var menuurl=menuurl.replace("http://"+menuurl.hostname, "").replace(/^\//, "")
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return (tabdropdown.currentpageurl==menuurl)
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},
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init:function(menuid, dselected){
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this.standardbody=(document.compatMode=="CSS1Compat")? document.documentElement : document.body //create reference to common "body" across doctypes
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var menuitems=document.getElementById(menuid).getElementsByTagName("a")
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for (var i=0; i<menuitems.length; i++){
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if (menuitems[i].getAttribute("rel")){
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var relvalue=menuitems[i].getAttribute("rel")
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document.getElementById(relvalue).firstlink=document.getElementById(relvalue).getElementsByTagName("a")[0]
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menuitems[i].onmouseover=function(e){
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var event=typeof e!="undefined"? e : window.event
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tabdropdown.dropit(this, event, this.getAttribute("rel"))
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}
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}
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if (dselected=="auto" && typeof setalready=="undefined" && this.isSelected(menuitems[i].href)){
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menuitems[i].parentNode.className+=" selected default"
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var setalready=true
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}
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else if (parseInt(dselected)==i)
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menuitems[i].parentNode.className+=" selected default"
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-
}
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}
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+
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}
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</script>
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<style>
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.ddcolortabs{
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padding: 0;
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width: 100%;
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background: transparent;
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voice-family: "\"}\"";
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voice-family: inherit;
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-
}
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-
 
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.ddcolortabs ul{
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font: normal 11px Arial, Verdana, sans-serif;
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margin:0;
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padding:0;
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list-style:none;
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}
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-
 
+
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.ddcolortabs li{
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display:inline;
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margin:0 2px 0 0;
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padding:0;
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text-transform:uppercase;
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}
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+
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.ddcolortabs a{
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float:left;
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color: white;
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background: black url(media/color_tabs_left.gif) no-repeat left top;
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margin:0 2px 0 0;
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padding:0 0 1px 3px;
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text-decoration:none;
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letter-spacing: 1px;
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}
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.ddcolortabs a span{
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float:left;
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display:block;
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background: transparent url(media/color_tabs_right.gif) no-repeat right top;
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padding: 4px 8px 2px 7px;
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}
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.ddcolortabs a span{
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float:none;
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}
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.ddcolortabs a:hover{
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background-color: #591f20;
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}
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.ddcolortabs a:hover span{
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background-color: #591f20;
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}
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.ddcolortabs .selected a, #ddcolortabs .selected a span{ /*currently selected tab*/
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background-color: #591f20;
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}
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.ddcolortabsline{
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clear: both;
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padding: 0;
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width: 100%;
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height: 8px;
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line-height: 8px;
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background: black;
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border-top: 1px solid #fff; /*Remove this to remove border between bar and tabs*/
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}
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/* ######### Style for Drop Down Menu ######### */
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.dropmenudiv_a{
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position:absolute;
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top: 0;
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border: 1px solid black; /*THEME CHANGE HERE*/
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border-top-width: 8px; /*Top border width. Should match height of .ddcolortabsline above*/
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border-bottom-width: 0;
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font:normal 12px Arial;
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-
line-height:18px;
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z-index:100;
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-
background-color: white;
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-
width: 200px;
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-
visibility: hidden;
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-
}
+
-
 
+
-
 
+
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.dropmenudiv_a a{
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width: auto;
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-
display: block;
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text-indent: 5px;
+
-
border-top: 0 solid #678b3f;
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-
border-bottom: 1px solid #678b3f; /*THEME CHANGE HERE*/
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padding: 2px 0;
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text-decoration: none;
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color: black;
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}
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* html .dropmenudiv_a a{ /*IE only hack*/
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width: 100%;
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}
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.dropmenudiv_a a:hover{ /*THEME CHANGE HERE*/
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-
background-color: #8a3c3d;
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-
color: white;
+
-
}
+
-
</style>
+
-
</head>
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-
 
+
-
<!-- CSS for Drop Down Tabs Menu #1 -->
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<link rel="stylesheet" type="text/css" href="ddcolortabs.css" />
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<div id="colortab" class="ddcolortabs">
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<ul>
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<li><a href="https://2008.igem.org/Team:Edinburgh" title="Home"><span>Home</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Project" title="Project" rel="dropmenu1_a"><span>The Project</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Team" title="Team" ><span>The Team</span></a></li>
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<li><a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=Edinburgh" title="Project" rel="dropmenu1_a"><span>BioBrick Parts</span></a></li>
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-
<li><a href="https://2008.igem.org/Team:Edinburgh/Modeling" title="Modelling" rel="dropmenu2_a"><span>Modelling</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Notebook" title="Notebook"><span>Notebook</span></a></li>
+
-
</ul>
+
-
</div>
+
-
<div class="ddcolortabsline">&nbsp;</div>
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-
<div id="dropmenu1_a" class="dropmenudiv_a">
+
-
<a href="https://2008.igem.org/Team:Edinburgh/Team">Overview</a>
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-
<a href="https://2008.igem.org/Team:Edinburgh/Team">Step1</a>
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-
<a href="https://2008.igem.org/Team:Edinburgh/Team">Step2</a>
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</div>
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<script type="text/javascript">
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//SYNTAX: tabdropdown.init("menu_id", [integer OR "auto"])
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tabdropdown.init("colortab", 3)
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-
</script>
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</html>
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=== Week 9 ===
=== Week 9 ===
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* Submitted M109 (pSb1A2+''crtB''+''crtI'') for sequencing as AH_109F and AH_109R using primers pSB1A2insF2 and pSB1A2insR2 respectively. (AH)
* Submitted M109 (pSb1A2+''crtB''+''crtI'') for sequencing as AH_109F and AH_109R using primers pSB1A2insF2 and pSB1A2insR2 respectively. (AH)
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* '''M127-M130''': Minipreps of pSB1A2+pCstA (1,2,3,4), '''M131-M134''': Minipreps of pSB1A2+''dxs''+''crtE'' (1,2,3,4), '''M135-M138''': Minipreps of pSB1A2+''dxs''+''lims1'' (1,2,3,4). All from plate 115. Note: M132 and M136 might be labelled wrong, the ethanol erased the writing on the tubes. Sorry! (OG)
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* '''M127-M130''': Minipreps of pSB1A2+''P<sub>cstA</sub>'' (1,2,3,4), '''M131-M134''': Minipreps of pSB1A2+''dxs''+''crtE'' (1,2,3,4), '''M135-M138''': Minipreps of pSB1A2+''dxs''+''lims1'' (1,2,3,4). All from plate 115. Note: M132 and M136 might be labelled wrong, the ethanol erased the writing on the tubes. Sorry! (OG)
* Double digests performed on M127-138 using EcoRI/PstI. (AH, OG)
* Double digests performed on M127-138 using EcoRI/PstI. (AH, OG)
* Transformation of L38 (pSB1A2-''crtE'') on '''Plate 116''' (100ml) and '''plate 117''' (900ml). (HX)
* Transformation of L38 (pSB1A2-''crtE'') on '''Plate 116''' (100ml) and '''plate 117''' (900ml). (HX)
* Ligation of P54 (''cex'') and P65 (''cenA'') into Edinbrick1, to make L39 (pSB1A2+''cex'') and L40 (pSB1A2+''cenA''). (Yan)
* Ligation of P54 (''cex'') and P65 (''cenA'') into Edinbrick1, to make L39 (pSB1A2+''cex'') and L40 (pSB1A2+''cenA''). (Yan)
-
* PCR for ''cenB'' ('''P68''') and ''cenC'' ('''P69''') from ''C. fimi'' genomic DNA: denaturing 1 min, annealing 56°C for 20s, extension 85s, with glycerol. Run on '''Gel 49'''. Results indicate failure. (AM)
+
* PCR for ''cenB'' ('''P68''') and ''cenC'' ('''P69''') from ''C. fimi'' genomic DNA: denaturing 1 min, annealing 56°C for 20s, extension 85s, with glycerol. Run on '''Gel 49'''. Results indicate failure. (AM)<br />
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<br />
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:::: '''[[Edinburgh/14_August_2008|Next Entry >]]'''

Latest revision as of 20:54, 29 October 2008

< Previous Entry

Week 9

Wednesday 13 August 08

  • Submitted M109 (pSb1A2+crtB+crtI) for sequencing as AH_109F and AH_109R using primers pSB1A2insF2 and pSB1A2insR2 respectively. (AH)
  • M127-M130: Minipreps of pSB1A2+PcstA (1,2,3,4), M131-M134: Minipreps of pSB1A2+dxs+crtE (1,2,3,4), M135-M138: Minipreps of pSB1A2+dxs+lims1 (1,2,3,4). All from plate 115. Note: M132 and M136 might be labelled wrong, the ethanol erased the writing on the tubes. Sorry! (OG)
  • Double digests performed on M127-138 using EcoRI/PstI. (AH, OG)
  • Transformation of L38 (pSB1A2-crtE) on Plate 116 (100ml) and plate 117 (900ml). (HX)
  • Ligation of P54 (cex) and P65 (cenA) into Edinbrick1, to make L39 (pSB1A2+cex) and L40 (pSB1A2+cenA). (Yan)
  • PCR for cenB (P68) and cenC (P69) from C. fimi genomic DNA: denaturing 1 min, annealing 56°C for 20s, extension 85s, with glycerol. Run on Gel 49. Results indicate failure. (AM)


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