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Team:Hawaii/Notebook/2008-08-26
From 2008.igem.org
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(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Ran RE digests on gel=== :<strong> Grace</strong> :* Gel did not resolve. Bands were convex, poor separation between b...) |
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:* BB-pRL1383a and J33207 in TB+amp<sub>100</sub> | :* BB-pRL1383a and J33207 in TB+amp<sub>100</sub> | ||
| + | ===Gel Purified=== | ||
| + | :<strong>Krystle</strong> | ||
| + | |||
| + | :* Ran overnight ligation products on a 3% agarose gel | ||
| + | ::''discussion: If we decide to do the 3A in two parts, we will have to re-restriction digest the first part before moving on'' | ||
| + | |||
| + | ===Transformation of ligated GFPf + TT=== | ||
| + | :<strong>Krystle</strong> | ||
| + | |||
| + | :*Performed transformation protocol with DB3.1 and GFPf + tt ligation from 08/25 | ||
| + | |||
| + | ===Ligation=== | ||
| + | <strong>Margaret</strong> | ||
| + | :*Overnight in 4°C | ||
| + | :*rep+pSB1A3 (1:1) | ||
| + | :*rep+pSB1A3 (1:3) | ||
| + | :*rep+pSB1A3 (1:6) | ||
| + | :*P1 lytic +pSB1A3 (1:1) | ||
| + | :*P1 lytic +pSB1A3 (1:3) | ||
| + | :*P1 lytic +pSB1A3 (1:6) | ||
| + | :*[Plac B0030]+pSB1A3 (1:3) | ||
| + | :*[Plac B0030]+pSB1A3 (1:6) | ||
| + | :*[Plac B0034]+pSB1A3 (1:6)**ran out of vector | ||
= Discussion = | = Discussion = | ||
Latest revision as of 03:31, 28 August 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Ran RE digests on gel
- Grace
- Gel did not resolve. Bands were convex, poor separation between bands. Bad buffer?
- Redid RE digests from yesterday
- Used PCR products of nir and J33207
Inoculated for plasmid prep
- Grace
- BB-pRL1383a and J33207 in TB+amp100
Gel Purified
- Krystle
- Ran overnight ligation products on a 3% agarose gel
- discussion: If we decide to do the 3A in two parts, we will have to re-restriction digest the first part before moving on
Transformation of ligated GFPf + TT
- Krystle
- Performed transformation protocol with DB3.1 and GFPf + tt ligation from 08/25
Ligation
Margaret
- Overnight in 4°C
- rep+pSB1A3 (1:1)
- rep+pSB1A3 (1:3)
- rep+pSB1A3 (1:6)
- P1 lytic +pSB1A3 (1:1)
- P1 lytic +pSB1A3 (1:3)
- P1 lytic +pSB1A3 (1:6)
- [Plac B0030]+pSB1A3 (1:3)
- [Plac B0030]+pSB1A3 (1:6)
- [Plac B0034]+pSB1A3 (1:6)**ran out of vector
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
