Latest revision as of 03:31, 28 August 2008

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Things we did today

Grace

Gel did not resolve. Bands were convex, poor separation between bands. Bad buffer?
Redid RE digests from yesterday

Grace

Krystle

discussion: If we decide to do the 3A in two parts, we will have to re-restriction digest the first part before moving on

Krystle

Margaret

Overnight in 4°C
rep+pSB1A3 (1:1)
rep+pSB1A3 (1:3)
rep+pSB1A3 (1:6)
P1 lytic +pSB1A3 (1:1)
P1 lytic +pSB1A3 (1:3)
P1 lytic +pSB1A3 (1:6)
[Plac B0030]+pSB1A3 (1:3)
[Plac B0030]+pSB1A3 (1:6)
[Plac B0034]+pSB1A3 (1:6)**ran out of vector

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

@@ Line 19: / Line 19: @@
 :* Ran overnight ligation products on a 3% agarose gel
-::*'''discussion: If we decide to do the 3A in two parts, we will have to re-restriction digest the first part before moving on'''
+::''discussion: If we decide to do the 3A in two parts, we will have to re-restriction digest the first part before moving on''
 ===Transformation of ligated GFPf + TT===
-:<strong>Krystle<strong>
+:<strong>Krystle</strong>
 :*Performed transformation protocol with DB3.1 and GFPf + tt ligation from 08/25
+===Ligation===
+<strong>Margaret</strong>
+:*Overnight in 4&deg;C
+:*rep+pSB1A3 (1:1)
+:*rep+pSB1A3 (1:3)
+:*rep+pSB1A3 (1:6)
+:*P1 lytic +pSB1A3 (1:1)
+:*P1 lytic +pSB1A3 (1:3)
+:*P1 lytic +pSB1A3 (1:6)
+:*[Plac B0030]+pSB1A3 (1:3)
+:*[Plac B0030]+pSB1A3 (1:6)
+:*[Plac B0034]+pSB1A3 (1:6)**ran out of vector
 = Discussion =