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Team:KULeuven/28 August 2008
From 2008.igem.org
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== Lab Work == | == Lab Work == | ||
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| + | [[image:gel-28-8a.jpg|right|thumb|350px|'''Gel 1''': Test ligation with PCR. All 4 colonies of K145151+pSB1A2 ligated properly, but only the first colony of K145001+pSB1A2 ligated properly]] | ||
| + | [[image:gel-28-8b.jpg|right|thumb|350px|'''Gel 2''': Digests of B0015, C0012, C0060, K145015 and C0040]] | ||
=== Wet Lab === | === Wet Lab === | ||
| - | + | The ligations with B0015 ([http://partsregistry.org/Part:BBa_C0040 C0040]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_C0060 C0060]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_C0012 C0012]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_K145015 K145015]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_K145001 K145001]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:BBa_B0015 B0015]) were electroporated again, but now they were plated on an ampicillin plate. | |
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| - | + | A glycerol stock was made of the succeeded ligations. | |
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| - | + | We ordered some new parts, because the modeling showed that a lot of things had to be changed. They arrived today and they were also plated. | |
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| + | The ligations of [http://partsregistry.org/Part:BBa_K145001 K145001] and [http://partsregistry.org/Part:BBa_K145151 K145151] with [http://partsregistry.org/Part:pSB1A2 pSB1A2] were miniprepped and then we did a PCR to test the ligation (primers VF/VR-2). See '''Gel 1'''. | ||
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| + | The digests of [http://partsregistry.org/Part:BBa_R0011 R0011], [http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23022 J23022], [http://partsregistry.org/Part:BBa_C0062 C0062] and [http://partsregistry.org/Part:BBa_B0033 B0033] were put on gel and purified. | ||
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| + | Some more digests were made, but this time we cut with ''Spe''I, ''Xba''I and ''Pst''I. We cut [http://partsregistry.org/Part:BBa_B0015 B0015] as insert (''Xba''I and ''Pst''I) and [http://partsregistry.org/Part:BBa_C0060 C0060], [http://partsregistry.org/Part:BBa_K145015 K145015], [http://partsregistry.org/Part:BBa_C0012 C0012] and [http://partsregistry.org/Part:BBa_C0040 C0040] were cut as vector (''Spe''I and ''Pst''I). See '''Gel 2'''. | ||
=== Dry Lab === | === Dry Lab === | ||
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| + | ==== Ethics ==== | ||
We did some more research on bioethics and human practice. | We did some more research on bioethics and human practice. | ||
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==== Modeling ==== | ==== Modeling ==== | ||
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Simulations of the different parts has continued. | Simulations of the different parts has continued. | ||
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Latest revision as of 12:18, 12 October 2008
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Contents |
Lab Work
Wet Lab
The ligations with B0015 ([http://partsregistry.org/Part:BBa_C0040 C0040]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_C0060 C0060]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_C0012 C0012]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_K145015 K145015]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_K145001 K145001]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:BBa_B0015 B0015]) were electroporated again, but now they were plated on an ampicillin plate.
A glycerol stock was made of the succeeded ligations.
We ordered some new parts, because the modeling showed that a lot of things had to be changed. They arrived today and they were also plated.
The ligations of [http://partsregistry.org/Part:BBa_K145001 K145001] and [http://partsregistry.org/Part:BBa_K145151 K145151] with [http://partsregistry.org/Part:pSB1A2 pSB1A2] were miniprepped and then we did a PCR to test the ligation (primers VF/VR-2). See Gel 1.
The digests of [http://partsregistry.org/Part:BBa_R0011 R0011], [http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23022 J23022], [http://partsregistry.org/Part:BBa_C0062 C0062] and [http://partsregistry.org/Part:BBa_B0033 B0033] were put on gel and purified.
Some more digests were made, but this time we cut with SpeI, XbaI and PstI. We cut [http://partsregistry.org/Part:BBa_B0015 B0015] as insert (XbaI and PstI) and [http://partsregistry.org/Part:BBa_C0060 C0060], [http://partsregistry.org/Part:BBa_K145015 K145015], [http://partsregistry.org/Part:BBa_C0012 C0012] and [http://partsregistry.org/Part:BBa_C0040 C0040] were cut as vector (SpeI and PstI). See Gel 2.
Dry Lab
Ethics
We did some more research on bioethics and human practice.
Modeling
Great Succes: we succeeded in modeling cell-multiplication in a Matlab-environment. This will be our starting point for modeling cell division and work in a multi-cell environment! [we did what the lab couldn't do]
Parameter checking has almost reached its final state.
Simulations of the different parts has continued.
