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Team:Chiba/protocol/ligation/dephosphorylation
From 2008.igem.org
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dephosphorylation | dephosphorylation | ||
| + | |||
| + | SAP: Alkaline Phosphatase (Shrimp) | ||
| + | |||
| + | *Mix | ||
| + | DNA fragment 1~10 pmol | ||
| + | Shrimp Alkaline Phosphatase (1~5 μ l) 1~5 U | ||
| + | 10X SAP Buffer 5 μ l | ||
| + | Sterilized distilled water up to 50 μ l | ||
| + | |||
| + | #Incubate at 37℃ for 15~30 min. | ||
| + | #Incubate at 65℃ for 15 min. (for inactivation by heat treatment) | ||
| + | #Purify the DNA with Zymo DNA Cleam&Concentrator Kit | ||
| + | |||
| + | READ:[[Team:Chiba/protocol/DNA_Purification/zymocleam|Zymo cleam]] | ||
Latest revision as of 03:09, 11 September 2008
dephosphorylation
SAP: Alkaline Phosphatase (Shrimp)
- Mix
DNA fragment 1~10 pmol Shrimp Alkaline Phosphatase (1~5 μ l) 1~5 U 10X SAP Buffer 5 μ l Sterilized distilled water up to 50 μ l
- Incubate at 37℃ for 15~30 min.
- Incubate at 65℃ for 15 min. (for inactivation by heat treatment)
- Purify the DNA with Zymo DNA Cleam&Concentrator Kit
READ:Zymo cleam
