"
Team:KULeuven/6 September 2008
From 2008.igem.org
(Difference between revisions)
(New page: {{:Team:KULeuven/Tools/Header}} == Lab Work == === Wet Lab === Busy day for a saturday ;) * Made some new electrocompetent TOP10 cells, we've got about 32 new aliquots of bacteria happy...) |
m |
||
| (5 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
| + | {{:Team:KULeuven/Tools/Styling}} | ||
{{:Team:KULeuven/Tools/Header}} | {{:Team:KULeuven/Tools/Header}} | ||
| + | |||
| + | {{:Team:KULeuven/Tools/New_Day/Date_Retriever}} | ||
== Lab Work == | == Lab Work == | ||
| Line 7: | Line 10: | ||
Busy day for a saturday ;) | Busy day for a saturday ;) | ||
* Made some new electrocompetent TOP10 cells, we've got about 32 new aliquots of bacteria happy to be fried by a thousand volts. | * Made some new electrocompetent TOP10 cells, we've got about 32 new aliquots of bacteria happy to be fried by a thousand volts. | ||
| - | * PCR with prefix-sufix primers on R1052 and the hybrid promoter. Gave some weird results. Will need to be looked at again. | + | * PCR with prefix-sufix primers on [http://partsregistry.org/Part:BBa_R1052 R1052] and the hybrid promoter. Gave some weird results. Will need to be looked at again. |
| + | * Made a couple digests: [http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_E0240 E0240], [http://partsregistry.org/Part:BBa_C0056 C0056]+[http://partsregistry.org/Part:BBa_B0015 B0015] and [http://partsregistry.org/Part:BBa_C0061 C0061]+[http://partsregistry.org/Part:BBa_B0015 B0015] were cut with ''Eco''RI, E0040 was cut with ''Spe''I and ''Pst''I, and [http://partsregistry.org/Part:BBa_B0015 B0015] was cut with ''Xba''I and ''Pst''I. They were put on gel and then purified. The results looked very good. | ||
| + | * Also made a digest of [http://partsregistry.org/Part:BBa_K145201 K145201], [http://partsregistry.org/Part:BBa_K145205 K145205], [http://partsregistry.org/Part:BBa_K145253 K145253], [http://partsregistry.org/Part:BBa_K145251 K145251] and [http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23022 J23022]+[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032], but then I forgot to load the Smartladder onto the gel. These digest were set up again (overnight reaction). Anyway, the gel already looked very promising ;-) | ||
| + | * Started some new ligations (mainly to test the new hybrid promoter): R0040+P0353, (R0040+B0032)+(C0062+B0015), (R0011+B0032)+(C0061+B0015) and E0040+B0015. | ||
| + | * We set up a PCR to test our ligations using the VF2/VR primers. | ||
| - | == | + | == Comic of the day == |
| - | = | + | <div class="center">http://www.phdcomics.com/comics/archive/phd090308s.gif</div> |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
Latest revision as of 01:41, 30 October 2008
dock/undock dropdown
| << return to notebook | return to homepage >> | ||
| < previous friday | ← yesterday | tomorrow → | next monday > |
Lab Work
Wet Lab
Busy day for a saturday ;)
- Made some new electrocompetent TOP10 cells, we've got about 32 new aliquots of bacteria happy to be fried by a thousand volts.
- PCR with prefix-sufix primers on [http://partsregistry.org/Part:BBa_R1052 R1052] and the hybrid promoter. Gave some weird results. Will need to be looked at again.
- Made a couple digests: [http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_E0240 E0240], [http://partsregistry.org/Part:BBa_C0056 C0056]+[http://partsregistry.org/Part:BBa_B0015 B0015] and [http://partsregistry.org/Part:BBa_C0061 C0061]+[http://partsregistry.org/Part:BBa_B0015 B0015] were cut with EcoRI, E0040 was cut with SpeI and PstI, and [http://partsregistry.org/Part:BBa_B0015 B0015] was cut with XbaI and PstI. They were put on gel and then purified. The results looked very good.
- Also made a digest of [http://partsregistry.org/Part:BBa_K145201 K145201], [http://partsregistry.org/Part:BBa_K145205 K145205], [http://partsregistry.org/Part:BBa_K145253 K145253], [http://partsregistry.org/Part:BBa_K145251 K145251] and [http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23022 J23022]+[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032], but then I forgot to load the Smartladder onto the gel. These digest were set up again (overnight reaction). Anyway, the gel already looked very promising ;-)
- Started some new ligations (mainly to test the new hybrid promoter): R0040+P0353, (R0040+B0032)+(C0062+B0015), (R0011+B0032)+(C0061+B0015) and E0040+B0015.
- We set up a PCR to test our ligations using the VF2/VR primers.
Comic of the day
http://www.phdcomics.com/comics/archive/phd090308s.gif
