Team:KULeuven/9 September 2008
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{{:Team:KULeuven/Tools/Header}} | {{:Team:KULeuven/Tools/Header}} | ||
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== Lab Work == | == Lab Work == | ||
=== Wet Lab === | === Wet Lab === | ||
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+ | * We MiniPrepped the ligations (K145150+E0240, R0040+P0353, E0040+B0015, K145254, K145150+B0034, K145013+pSB1A2, C0062). Then we made digests of them. | ||
+ | * The colonies resulting from the electroporation were streaked out. K145280 colonies were not fluorescent, K145279 were. Maybe this is because of the long lifetime of GFP. | ||
+ | * We did a PCR to test the ligations. Only K145201+K145205, K145201+R0040+E0240, R0040+B0033+C0056+B0015 seemed fine. We made a liquid culture of them. | ||
+ | * We made a glycerolstock of some successful ligations. | ||
=== Dry Lab === | === Dry Lab === | ||
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==== Modeling ==== | ==== Modeling ==== | ||
- | Finished the MultiCell Toolbox and edited its Wiki-page. | + | Finished the MultiCell Toolbox and edited its [https://2008.igem.org/Team:KULeuven/Software/MultiCell Wiki-page]. |
==== Wiki ==== | ==== Wiki ==== | ||
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[[Image:Views.PNG|center]] | [[Image:Views.PNG|center]] | ||
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Latest revision as of 19:36, 28 October 2008
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Contents |
Lab Work
Wet Lab
- We MiniPrepped the ligations (K145150+E0240, R0040+P0353, E0040+B0015, K145254, K145150+B0034, K145013+pSB1A2, C0062). Then we made digests of them.
- The colonies resulting from the electroporation were streaked out. K145280 colonies were not fluorescent, K145279 were. Maybe this is because of the long lifetime of GFP.
- We did a PCR to test the ligations. Only K145201+K145205, K145201+R0040+E0240, R0040+B0033+C0056+B0015 seemed fine. We made a liquid culture of them.
- We made a glycerolstock of some successful ligations.
Dry Lab
Modeling
Finished the MultiCell Toolbox and edited its Wiki-page.
Wiki
Last edits were conducted to the picture gallery, so it doesn't look like... well you know what, no more. Wiki finished.
Remarks
We discovered on the iGEM - Statistics page that our website is ranked n°2 in the list of Most Viewed Pages (with a n°1 place for the iGEM home page).