Latest revision as of 01:43, 21 September 2008

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Things we did today

Grace

Krystle

Gel used to verify the insertion of Promoter and RBS in front of aada(prl&bb).

Margaret

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

@@ Line 13: / Line 13: @@
 ::* 3A: nir+rbs+pilA+GFPf12 # 2, 6
 ::* 3A: nir+rbs+pilA+GFPf13 # 4, 6, 8, 10
+===Gel Purification===
+:<strong>Krystle</strong>
+:* gel purified restriction digest products of:
+:** nir
+:** pilA
+:** slr
+:** pSB1A3
+:** GFPf + tt #8
+:** GFPf + tt #17
+:** GFPf + tt #21
 ===Gel Verify constructs===
+[[Image:gel_veri_9_16.jpg|right|thumb|200px|Gel used to verify the insertion of Promoter and RBS in front of aada(prl&bb).]]
 :<strong>Margaret</strong>
-[[Image:gel_veri_9_16.jpg|right|thumb|300px|Gel used to verify the insertion of Promoter and RBS in front of aada(prl&bb).]]
 :*verified constructs: ap36, ab92, ab94, ab95, ab96, ab98
 :*verified constructs will be gel purified and used in a ligation.
 = Discussion =