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Team:Hawaii/Notebook/2008-09-16
From 2008.igem.org
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::* 3A: nir+rbs+pilA+GFPf12 # 2, 6 | ::* 3A: nir+rbs+pilA+GFPf12 # 2, 6 | ||
::* 3A: nir+rbs+pilA+GFPf13 # 4, 6, 8, 10 | ::* 3A: nir+rbs+pilA+GFPf13 # 4, 6, 8, 10 | ||
| + | |||
| + | ===Gel Purification=== | ||
| + | :<strong>Krystle</strong> | ||
| + | :* gel purified restriction digest products of: | ||
| + | :** nir | ||
| + | :** pilA | ||
| + | :** slr | ||
| + | :** pSB1A3 | ||
| + | :** GFPf + tt #8 | ||
| + | :** GFPf + tt #17 | ||
| + | :** GFPf + tt #21 | ||
===Gel Verify constructs=== | ===Gel Verify constructs=== | ||
Latest revision as of 01:43, 21 September 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Plasmid prep
- Grace
- 5 ml plasmid prep of:
- BB-pRL1383a
- 3A: nir+rbs+slr1 #2, 3
- 3A: nir+rbs+pilA #6
- 3A: nir+rbs+slr1+GFPf #10
- 3A: nir+rbs+pilA+GFPf12 # 2, 6
- 3A: nir+rbs+pilA+GFPf13 # 4, 6, 8, 10
Gel Purification
- Krystle
- gel purified restriction digest products of:
- nir
- pilA
- slr
- pSB1A3
- GFPf + tt #8
- GFPf + tt #17
- GFPf + tt #21
- gel purified restriction digest products of:
Gel Verify constructs
- Margaret
- verified constructs: ap36, ab92, ab94, ab95, ab96, ab98
- verified constructs will be gel purified and used in a ligation.
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
