Latest revision as of 22:30, 3 October 2008

Birds Eye View Team	Projects	Events	Resources
Sponsors	Experiments	Milestones	Protocols
	Notebook (t)	Meetings (t)

Things we did today

Grace

Construct	Colonies
nir+rbs+GFP + tt	3
nir + rbs+GFP+tt1	1
plac + rbs+GFP+tt1	1
nir + rbs+GFP+tt2	5
plac + rbs+GFP+tt2	0
pSB1A3 to self (- control)	0
J33207 + pRL1383a (plated on IPTG+Xgal)	31
J33207 + pRL1383a (1:10) (plated on Xgal)	0
pRL1383a to self (+ control)	0
J33207 plasmid (+ control, plated on IPTG+Xgal)	162 (all blue!)
pRL1383a plasmid (+ control)	0
no DNA	0

EtBr stained 2% agarose gel ran at 95V for 1.5 hours. Two microliters of PCR product were loaded into each well.

Margaret, Krystle, Grace

Reviewed Margaret's presentation for Dr. Lee and outlined presentation for Boston.

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

@@ Line 5: / Line 5: @@
 ===Verification of Transformants===
 :<strong> Grace</strong>
-[[Image:092608colonyPCR.jpg|right|thumb|250px|EtBr stained 2% agarose gel ran at 95V for 1.5 hours. Two microliters of PCR product were loaded into each well.]]
 {|class=wikitable border=1 align=center
 !Construct
@@ Line 29: / Line 29: @@
 |-
 |align=center|J33207 + pRL1383a (plated on IPTG+Xgal)
-|align=center|
+|align=center|31
 |-
 |align=center|J33207 + pRL1383a (1:10) (plated on Xgal)
-|align=center|
+|align=center|0
 |-
 |align=center|pRL1383a to self (+ control)
-|align=center|
+|align=center|0
 |-
 |align=center|J33207 plasmid (+ control, plated on IPTG+Xgal)
@@ Line 41: / Line 41: @@
 |-
 |align=center|pRL1383a plasmid (+ control)
-|align=center|
+|align=center|0
 |-
 |align=center|no DNA
@@ Line 47: / Line 47: @@
 |}
 :* Colony PCR of transformants
+::* Bands too small for all secretion constructs
+::* BB-pRL1383a bands all ~875 (expected size = 850bp). =D!
+:* Restreaked BB-pRL1383a colonies #1, 3, 13, 18
+[[Image:092608colonyPCR.jpg|center|thumb|700px|EtBr stained 2% agarose gel ran at 95V for 1.5 hours. Two microliters of PCR product were loaded into each well.]]
 == Drylab Work ==