|
|
(One intermediate revision not shown) |
Line 2: |
Line 2: |
| {{:Team:KULeuven/Tools/Scripting}} | | {{:Team:KULeuven/Tools/Scripting}} |
| {{:Team:KULeuven/Tools/Header}} | | {{:Team:KULeuven/Tools/Header}} |
- |
| |
- | == Submitted Parts ==
| |
- |
| |
- |
| |
- | {|
| |
- | |- style="background: #003E81;color:white;width:100%;"
| |
- | ! colspan="6" style="background #003E81" | Submitted parts: Listing
| |
- | |- style="background: #99CCFF; text-align: center;"
| |
- | |width=90| '''Part number'''
| |
- | |width=90| '''Replaced by'''
| |
- | |wodth=470| '''Description'''
| |
- | |width=90| '''Type'''
| |
- | |width=110| '''Component'''
| |
- | |width=90| '''Status'''
| |
- | |- style="background:#e0ffff; color:grey"
| |
- | | '''K145000'''
| |
- | | '''K145100'''
| |
- | | eCFP with LVA tag under OmpR control
| |
- | | Part
| |
- | | Output
| |
- | | Discarded
| |
- | |- style="background:#e0ffff; color:grey"
| |
- | | '''K145001'''
| |
- | | /
| |
- | | T7 RNA polymerase
| |
- | | Part
| |
- | | ''(nowhere)''
| |
- | | Discarded
| |
- | |- style="background:#e0ffff; color:grey"
| |
- | | '''K145002'''
| |
- | | /
| |
- | | T7 RNA Polymerase under OmpF control
| |
- | | Composite part
| |
- | | ''(nowhere)''
| |
- | | Discarded
| |
- | |- style="background:#e0ffff; color:grey"
| |
- | | '''K145003'''
| |
- | | '''K145102'''
| |
- | | RiboKey 3d under control of T7 RNA polymerase promotor
| |
- | | Composite part
| |
- | | ''(nowhere)''
| |
- | | Discarded
| |
- | |- style="background:#e0ffff; color:grey"
| |
- | | '''K145004'''
| |
- | | '''K145104'''
| |
- | | T7 PoPS + RiboLock generate LacI Repressor. LacI Repressor represses transcription of LuxI from pLac.
| |
- | | Composite part
| |
- | | Inverter
| |
- | | Discarded
| |
- | |- style="background:#e0ffff; color:grey"
| |
- | | '''K145005'''
| |
- | | /
| |
- | | cI under control of a dual promotor and a RiboLock.
| |
- | | Composite part
| |
- | | Pulse generator
| |
- | | Discarded
| |
- | |- style="background:#e0ffff; color:grey"
| |
- | | '''K145006'''
| |
- | | '''K145106'''
| |
- | | Lactonase under PRM control
| |
- | | Composite part
| |
- | | ''(nowhere)''
| |
- | | Discarded
| |
- | |- style="background:#e0ffff; color:grey"
| |
- | | '''K145007'''
| |
- | | '''K145107'''
| |
- | | Pulse Generator
| |
- | | Composite part
| |
- | | ''(nowhere)''
| |
- | | Discarded
| |
- | |- style="background:#e0ffff; color:grey"
| |
- | | '''K145008'''
| |
- | | '''K145108'''
| |
- | | Constitutive production of LuxR
| |
- | | Composite part
| |
- | | ''(nowhere)''
| |
- | | Discarded
| |
- | |- style="background:#e0ffff; color:grey"
| |
- | | '''K145009'''
| |
- | | '''K145109'''
| |
- | | ccdB cell death gene under control of an activating Lux PR
| |
- | | Composite part
| |
- | | ''(nowhere)''
| |
- | | Discarded
| |
- | |- style="background:#e0ffff; color:grey"
| |
- | | '''K145010'''
| |
- | | '''K145110'''
| |
- | | Killing switch
| |
- | | Composite part
| |
- | | ''(nowhere)''
| |
- | | Discarded
| |
- | |- style="background:#e0ffff; color:grey"
| |
- | | '''K145011'''
| |
- | | /
| |
- | | Light sensor to provide dummy input for the system, copy of '''BBa_M30109'''
| |
- | | Composite part
| |
- | | Input
| |
- | | Deleted
| |
- | |- style="background:#e0ffff; color:grey"
| |
- | | '''K145012'''
| |
- | | /
| |
- | | N-terminal degradation tag
| |
- | | Part
| |
- | | Filter
| |
- | | Not used
| |
- | |- style="background:#e0ffff; color:black"
| |
- | | '''K145013'''
| |
- | | /
| |
- | | antisense LuxI
| |
- | | Part
| |
- | | Memory
| |
- | | In use
| |
- | |- style="background:#e0ffff; color:black"
| |
- | | '''K145014'''
| |
- | | /
| |
- | | T7 RNA Polymerase with N-terminal UmuD degradation tag
| |
- | | Part
| |
- | | Filter
| |
- | | In use
| |
- | |- style="background:#e0ffff; color:black"
| |
- | | '''K145015'''
| |
- | | /
| |
- | | GFP with LVA tag
| |
- | | Part
| |
- | | Ouput
| |
- | | In use
| |
- | |- style="background:#e0ffff; color:black"
| |
- | | '''K145016'''
| |
- | | /
| |
- | | Intermediate TetR-generator
| |
- | | Composite part
| |
- | | Input
| |
- | | In use
| |
- | |- style="background:#e0ffff; color:grey"
| |
- | | '''K145100'''
| |
- | | '''K145205'''
| |
- | | OmpF PoPS -> GFP
| |
- | | Composite part
| |
- | | Output
| |
- | | Discarded
| |
- | |- style="background:#e0ffff; color:grey"
| |
- | | '''K145101'''
| |
- | | '''K145110'''
| |
- | | OmpF PoPS -> deactivate constitutive antisense LuxI
| |
- | | Composite part
| |
- | | Memory
| |
- | | Discarded
| |
- | |- style="background:#e0ffff; color:grey"
| |
- | | '''K145102'''
| |
- | | '''K145215'''
| |
- | | OmpF PoPS -> RiboKey3d
| |
- | | Composite part
| |
- | | Filter
| |
- | | Discarded
| |
- | |- style="background:#e0ffff; color:grey"
| |
- | | '''K145103'''
| |
- | | '''K145216'''
| |
- | | T7 with UmuD derived tag under constitutive promotor + RiboLock3d
| |
- | | Composite Part
| |
- | | Filter
| |
- | | Discarded
| |
- | |- style="background:#e0ffff; color:grey"
| |
- | | '''K145104'''
| |
- | | '''K145220'''
| |
- | | T7 PoPS + RiboLock -> LacI -> inhibition of LuxI
| |
- | | Composite part
| |
- | | Inverter
| |
- | | Discarded
| |
- | |- style="background:#e0ffff; color:grey"
| |
- | | '''K145105'''
| |
- | | '''K145225'''
| |
- | | cI under T7 and P<sub>R</sub> dual promotor
| |
- | | Composite part
| |
- | | Pulse Generator
| |
- | | Discarded
| |
- | |- style="background:#e0ffff; color:black"
| |
- | | '''K145106'''
| |
- | | /
| |
- | | cI P<sub>RM</sub> PoPS -> Lactonase
| |
- | | Composite part
| |
- | | Pulse Generator
| |
- | | Unknown
| |
- | |- style="background:#e0ffff; color:grey"
| |
- | | '''K145107'''
| |
- | | '''K145117'''
| |
- | | Lactonase pulse generator
| |
- | | Composite part
| |
- | | Pulse Generator
| |
- | | Discarded
| |
- | |- style="background:#e0ffff; color:black"
| |
- | | '''K145108'''
| |
- | | /
| |
- | | Constitutive PoPS -> LuxR
| |
- | | Composite part
| |
- | | Cell death
| |
- | | Unknown
| |
- | |- style="background:#e0ffff; color:black"
| |
- | | '''K145109'''
| |
- | | /
| |
- | | Hybrid P22 c2 - luxP<sub>R</sub> PoPS -> ccdB
| |
- | | Composite part
| |
- | | Cell death
| |
- | | Unknown
| |
- | |- style="background:#e0ffff; color:black"
| |
- | | '''K145110'''
| |
- | | /
| |
- | | Cell death mechanism
| |
- | | Composite part
| |
- | | Cell death
| |
- | | Unknown
| |
- | |- style="background:#e0ffff; color:black"
| |
- | | '''K145111'''
| |
- | | /
| |
- | | OmpF PoPS -> deactivate constitutive antisense LuxI
| |
- | | Composite part
| |
- | | Memory
| |
- | | Unknown
| |
- | |- style="background:#e0ffff; color:black"
| |
- | | '''K145112'''
| |
- | | /
| |
- | | cI under T7 and PR<sub>R</sub> dual promotor
| |
- | | Composite part
| |
- | | Pulse generator
| |
- | | Unknown
| |
- | |- style="background:#e0ffff; color:black"
| |
- | | '''K145113'''
| |
- | | /
| |
- | | Constitutive PoPS -> TetR
| |
- | | ?
| |
- | | ?
| |
- | | Unknown
| |
- | |- style="background:#e0ffff; color:black"
| |
- | | '''K145115'''
| |
- | | /
| |
- | | cI under T7 and PRR dual promotor
| |
- | | Composite part
| |
- | | Pulse generator
| |
- | | Unknown
| |
- | |- style="background:#e0ffff; color:black"
| |
- | | '''K145117'''
| |
- | | /
| |
- | | Lactonase pulse generator
| |
- | | Composite part
| |
- | | Pulse generator
| |
- | | Unknown
| |
- | |- style="background:#e0ffff; color:black"
| |
- | | '''K145150'''
| |
- | | /
| |
- | | Hybrid promoter: HSL-LuxR activated, P22 C2 repressed
| |
- | | Part
| |
- | | CellDeath
| |
- | | In use
| |
- | |- style="background:#e0ffff; color:black"
| |
- | | '''K145151'''
| |
- | | /
| |
- | | ccdB coding region
| |
- | | Part
| |
- | | CellDeath
| |
- | | In use
| |
- | |- style="background:#e0ffff; color:black"
| |
- | | '''K145152'''
| |
- | | /
| |
- | | Hybrid promoter: P22 c2 , LacI NOR gate
| |
- | | Part
| |
- | | ?
| |
- | | Unknown
| |
- | |- style="background:#e0ffff; color:black"
| |
- | | '''K145200'''
| |
- | | /
| |
- | | INPUT lightsensor (DISCARDED)
| |
- | | Composite Part
| |
- | | Input
| |
- | | Unknown
| |
- | |- style="background:#e0ffff; color:black"
| |
- | | '''K145201'''
| |
- | | /
| |
- | | INPUT TetR generator
| |
- | | Composite Part
| |
- | | INPUT
| |
- | | Unknown
| |
- | |- style="background:#e0ffff; color:black"
| |
- | | '''K145205'''
| |
- | | /
| |
- | | OUTPUT GFP under TetR pomoter
| |
- | | Composite Part
| |
- | | OUTPUT
| |
- | | Unknown
| |
- | |- style="background:#e0ffff; color:black"
| |
- | | '''K145210'''
| |
- | | /
| |
- | | MEMORY
| |
- | | Composite Part
| |
- | | MEMORY
| |
- | | Unknown
| |
- | |- style="background:#e0ffff; color:black"
| |
- | | '''K145211'''
| |
- | | /
| |
- | | MEMORY OUTPUT asLuxI
| |
- | | Composite Part
| |
- | | MEMORY
| |
- | | Unknown
| |
- | |- style="background:#e0ffff; color:black"
| |
- | | '''K145215'''
| |
- | | /
| |
- | | FILTER Key
| |
- | | Composite Part
| |
- | | FILTER
| |
- | | Unknown
| |
- | |- style="background:#e0ffff; color:black"
| |
- | | '''K145216'''
| |
- | | /
| |
- | | FILTER T7 RNA pol
| |
- | | Composite Part
| |
- | | FILTER
| |
- | | Unknown
| |
- | |- style="background:#e0ffff; color:black"
| |
- | | '''K145217'''
| |
- | | /
| |
- | | FILTER Complete
| |
- | | Composite Part
| |
- | | FILTER
| |
- | | Unknown
| |
- | |- style="background:#e0ffff; color:black"
| |
- | | '''K145220'''
| |
- | | /
| |
- | | INVERTED TIMER
| |
- | | Composite Part
| |
- | | INVERTIMER
| |
- | | Unknown
| |
- | |- style="background:#e0ffff; color:black"
| |
- | | '''K145225'''
| |
- | | /
| |
- | | RESET lactonase
| |
- | | Composite Part
| |
- | | RESET
| |
- | | Unknown
| |
- | |- style="background:#e0ffff; color:black"
| |
- | | '''K145230'''
| |
- | | /
| |
- | | CELL DEATH
| |
- | | Composite Part
| |
- | | CELLDEATH
| |
- | | Unknown
| |
- | |}
| |
- |
| |
- | ==The Big Scheme of Used Parts==
| |
- |
| |
- | [https://static.igem.org/mediawiki/2008/6/6d/THE_BIG_SCHEME.xls The Big Scheme]
| |