Team:Caltech/Protocols/MIC Assay

From 2008.igem.org

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# Grow one 5 ml culture to saturation at 37C with shaking in the indicated media and antibiotics as necessary.
# Grow one 5 ml culture to saturation at 37C with shaking in the indicated media and antibiotics as necessary.
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# Back diluted parent culture 1:50 into 5 ml of the same media and antibiotics plus differing amounts of hydrogen peroxide and let shake at 37C overnight.  
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# Back dilute parent culture 1:50 into 5 ml of the same media and antibiotics plus differing amounts of hydrogen peroxide and let shake at 37C overnight.  
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# The MIC was taken to be the concentration of hydrogen peroxide necessary to prevent overnight turbid growth.
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# Record MIC as the concentration of hydrogen peroxide necessary to prevent overnight turbid growth.
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Measuring Minimum Inhibitory Concentration


  1. Grow one 5 ml culture to saturation at 37C with shaking in the indicated media and antibiotics as necessary.
  2. Back dilute parent culture 1:50 into 5 ml of the same media and antibiotics plus differing amounts of hydrogen peroxide and let shake at 37C overnight.
  3. Record MIC as the concentration of hydrogen peroxide necessary to prevent overnight turbid growth.
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