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Revision as of 18:41, 20 October 2008 (view source) Gracek (Talk | contribs) (→Wetlab work) ← Older edit		Latest revision as of 01:02, 30 October 2008 (view source) Ksalazar (Talk | contribs) (→Wetlab work)
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	:<strong>Grace</strong>		:<strong>Grace</strong>
-	== Drylab Work ==	+	===PCR of J04430 (lac+rbs+GFP+tt+tt)===
		+	:<strong> Grace</strong>
-	===Name of Task===	+	:* Will act as (-) control for GFP secretion in cyanos
-	:<strong> name of person/people who performed the task</strong>	+
-	:* Summary of task and what was done. Link to experiment for detailed notes if necessary.	+	===Overnight RE digest===
-	:* e.g. read through papers, worked on proposal, etc.	+	:<strong> Grace</strong>
		+	:* EcoRI and PstI digest of BBpRL1383a and J04430
		+
		+	===Made LB+sp<sub>100</sub> plates ===
		+	:<strong>Grace </strong>
		+
		+	===Prepped for sequencing===
		+	:<strong>Krystle</strong>
		+	:* Sent out correct-sized product to CORE for sequencing
		+	::* nir+rbs
		+	::* slr+gfpf
		+
		+	===Construction of GFP secretion device===
		+	:<strong>Krystle</strong>
		+	:* Restriction digested additional PCR product
		+	::*nir+rbs with EcoRI and SpeI
	= Discussion =		= Discussion =

---

Latest revision as of 01:02, 30 October 2008

Birds Eye  View Team	Projects	Events	Resources
Sponsors	Experiments	Milestones	Protocols
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Things we did today

Wetlab work

Large scale plasmid prep of BB-pRL1383a (blue)

Grace

PCR of J04430 (lac+rbs+GFP+tt+tt)

Grace

• Will act as (-) control for GFP secretion in cyanos

Overnight RE digest

Grace

• EcoRI and PstI digest of BBpRL1383a and J04430

Made LB+sp₁₀₀ plates

Grace

Prepped for sequencing

Krystle

• Sent out correct-sized product to CORE for sequencing

• nir+rbs
• slr+gfpf

Construction of GFP secretion device

Krystle

• Restriction digested additional PCR product

• nir+rbs with EcoRI and SpeI

Discussion

Quote of the Day

Garbage truck dripping E. coli... this has got to stop. - Roswell Mayor, http://www.youtube.com/watch?v=f7mEb-p4F44 (ok maybe we should start taking Amp + Kan + Sm + Sp before going to the bathroom)

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

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