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Team:Hawaii/Notebook/2008-10-20
From 2008.igem.org
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===Large scale plasmid prep of BB-pRL1383a (blue)=== | ===Large scale plasmid prep of BB-pRL1383a (blue)=== | ||
:<strong>Grace</strong> | :<strong>Grace</strong> | ||
| - | |||
| - | |||
===PCR of J04430 (lac+rbs+GFP+tt+tt)=== | ===PCR of J04430 (lac+rbs+GFP+tt+tt)=== | ||
| Line 12: | Line 10: | ||
:* Will act as (-) control for GFP secretion in cyanos | :* Will act as (-) control for GFP secretion in cyanos | ||
| + | |||
| + | ===Overnight RE digest=== | ||
| + | :<strong> Grace</strong> | ||
| + | |||
| + | :* EcoRI and PstI digest of BBpRL1383a and J04430 | ||
| + | |||
| + | ===Made LB+sp<sub>100</sub> plates === | ||
| + | :<strong>Grace </strong> | ||
| + | |||
| + | ===Prepped for sequencing=== | ||
| + | :<strong>Krystle</strong> | ||
| + | :* Sent out correct-sized product to CORE for sequencing | ||
| + | ::* nir+rbs | ||
| + | ::* slr+gfpf | ||
| + | |||
| + | ===Construction of GFP secretion device=== | ||
| + | :<strong>Krystle</strong> | ||
| + | :* Restriction digested additional PCR product | ||
| + | ::*nir+rbs with EcoRI and SpeI | ||
= Discussion = | = Discussion = | ||
Latest revision as of 01:02, 30 October 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Large scale plasmid prep of BB-pRL1383a (blue)
- Grace
PCR of J04430 (lac+rbs+GFP+tt+tt)
- Grace
- Will act as (-) control for GFP secretion in cyanos
Overnight RE digest
- Grace
- EcoRI and PstI digest of BBpRL1383a and J04430
Made LB+sp100 plates
- Grace
Prepped for sequencing
- Krystle
- Sent out correct-sized product to CORE for sequencing
- nir+rbs
- slr+gfpf
Construction of GFP secretion device
- Krystle
- Restriction digested additional PCR product
- nir+rbs with EcoRI and SpeI
Discussion
Quote of the Day
Garbage truck dripping E. coli... this has got to stop. - Roswell Mayor, http://www.youtube.com/watch?v=f7mEb-p4F44 (ok maybe we should start taking Amp + Kan + Sm + Sp before going to the bathroom)
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
