Latest revision as of 01:05, 30 October 2008

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Things we did today

EtBr stained 0.8% agarose gel ran at 60V for 90 minutes. Thirty microliters of the RE digest reaction were loaded into each well.

EtBr stained 0.8% agarose gel ran at 60V for 90 minutes. Ten microliters of PCR product were loaded.

Grace

Grace

Made LB+sp_variable plates with 50 μl 20X X-gal
Transformed 100 μl DH5α with 2 μl BB-pRL1383a (4 ng)
Plated 50 μl cells + SOB on each LB+sp plate; plated 20 μl untransformed cells + SOB on each control plate
Incubated at 37C for 2 days

Krystle

Loaded all of restriction digest into a 2% gel, which appeared blank after running for 1.5 hours

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

@@ Line 20: / Line 20: @@
 :* Plated 50 &mu;l cells + SOB on each LB+sp plate; plated 20 &mu;l untransformed cells + SOB on each control plate
 :* Incubated at 37C for 2 days
+===Construction of GFP secretion device===
+:<strong>Krystle</strong>
+:* Gel purified overnight restriction digested nir+rbs
+::* Loaded all of restriction digest into a 2% gel, which appeared blank after running for 1.5 hours
+:* Re-PCR nir+rbs and slr+gfpf from plasmid prep
+:* Restriction digest
+::*nir+rbs with EcoRI and SpeI
+::*slr+gfpf with XbaI and PstI
 = Discussion =