Team:Heidelberg/Notebook/Sensing Group/Notebook/7thweek

From 2008.igem.org

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</li>
</li>
<li>
<li>
-
<a href="https://2008.igem.org/Team:Heidelberg/Chemotaxis" style="color: white">Modeling<!--[if gt IE 6]><!--></a><!--<![endif]-->
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<a href="https://2008.igem.org/Team:Heidelberg/Modeling" style="color: white">Modeling<!--[if gt IE 6]><!--></a><!--<![endif]-->
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<ul class="DropDownMenu" id="MB1-DDM3">
<ul class="DropDownMenu" id="MB1-DDM3">
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</li>
</li>
<li>
<li>
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<a href="https://2008.igem.org/Team:Heidelberg/Notebook/Sensing_Group/Notebook" style="color: white">Notebook<!--[if gt IE 6]><!--></a><!--<![endif]-->
+
<a href="https://2008.igem.org/Team:Heidelberg/Notebook/Overview" style="color: white">Notebook<!--[if gt IE 6]><!--></a><!--<![endif]-->
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<ul class="DropDownMenu" id="MB1-DDM5">
<ul class="DropDownMenu" id="MB1-DDM5">
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</li>
</li>
<li style="width: 160px">
<li style="width: 160px">
-
<a href="https://2008.igem.org/Team:Heidelberg/Project/General_information" style="color: white">Human Practice<!--[if gt IE 6]><!--></a><!--<![endif]-->
+
<a href="https://2008.igem.org/Team:Heidelberg/Human_Practice/Project_Overview" style="color: white">Human Practice<!--[if gt IE 6]><!--></a><!--<![endif]-->
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<ul class="DropDownMenu" id="MB1-DDM4">
<ul class="DropDownMenu" id="MB1-DDM4">
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                               <li><a href="https://2008.igem.org/Team:Heidelberg/Human_Practice/Surveys"><span><span>Surveys</span></span></a></li>
                               <li><a href="https://2008.igem.org/Team:Heidelberg/Human_Practice/Surveys"><span><span>Surveys</span></span></a></li>
                               <li><a href="https://2008.igem.org/Team:Heidelberg/Human_Practice/Open_Day"><span><span>Open Day</span></span></a></li>
                               <li><a href="https://2008.igem.org/Team:Heidelberg/Human_Practice/Open_Day"><span><span>Open Day</span></span></a></li>
-
                               <li><a href="https://2008.igem.org/Team:Heidelberg/Human_Practice/Nobel_Prize"><span><span>Nobel Prize</span></span></a></li>
+
                               <li><a href="https://2008.igem.org/Team:Heidelberg/Human_Practice/Nobel_Prize"><span><span>Nobel Prize</span></span></a></li>
 +
</ul>
</ul>
<!--[if lte IE 6]></td></tr></table></a><![endif]-->
<!--[if lte IE 6]></td></tr></table></a><![endif]-->
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== Tuesday, 09/16/2008 ==
== Tuesday, 09/16/2008 ==
-
* Digestion of Fusion 1+2 with PstI
+
* Digestion of Fusion 1+2 with PstI (NEBuffer 3 + BSA)
[[Image:HD 080916-F1 2 Digestion PstI.png|center|thumb|400px|Digestion of Fusion constructs with PstI. All, except Fusion 1 colony 2 of NcoI/NdeI cloning, seem to be positive]]
[[Image:HD 080916-F1 2 Digestion PstI.png|center|thumb|400px|Digestion of Fusion constructs with PstI. All, except Fusion 1 colony 2 of NcoI/NdeI cloning, seem to be positive]]
* F1+2 2,3 (KpnI), F1+2 1,3 (NdeI) sent to sequencing @ GATC
* F1+2 2,3 (KpnI), F1+2 1,3 (NdeI) sent to sequencing @ GATC
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== Wednesday, 09/17/2008 ==
== Wednesday, 09/17/2008 ==
-
* Digestion of LuxP (Glycerol-Stock and cultures from Bench) with NcoI to check if correct
+
[[Image:HD 080917-LuxP Digestion NcoI.png|right|thumb|200px|Digestion of LuxP  from glycerol stock and from bench culture. Both are positive.]]
-
[[Image:HD 080917-LuxP Digestion NcoI.png|center|thumb|400px|Digestion of LuxP  from glycerol stock and from bench culture. Both are positive.]]
+
* Digestion of LuxP (Glycerol-Stock and cultures from Bench) with NcoI (NEBuffer 3) to check if correct
* O/N cultures of LuxP cells from Glycerol stock
* O/N cultures of LuxP cells from Glycerol stock
 +
 +
<br style="clear: both;"/>
== Thursday, 09/18/2008 ==
== Thursday, 09/18/2008 ==

Latest revision as of 15:38, 27 October 2008

Back to the overview


Contents

Tuesday, 09/16/2008

  • Digestion of Fusion 1+2 with PstI (NEBuffer 3 + BSA)
Digestion of Fusion constructs with PstI. All, except Fusion 1 colony 2 of NcoI/NdeI cloning, seem to be positive
  • F1+2 2,3 (KpnI), F1+2 1,3 (NdeI) sent to sequencing @ GATC 
F2 NdeI #3, F1 NdeI #1, F1 KpnI #2 seem to be correct

Wednesday, 09/17/2008

Digestion of LuxP from glycerol stock and from bench culture. Both are positive.
  • Digestion of LuxP (Glycerol-Stock and cultures from Bench) with NcoI (NEBuffer 3) to check if correct
  • O/N cultures of LuxP cells from Glycerol stock


Thursday, 09/18/2008

  • Miniprep of LuxP
  • Cloning of LuxP into Fusion constructs with SalI/NotI
  • Transformation into DH5a competent cells

Friday, 09/19/2008

nothing to report

Go to 8th week

Retrieved from "http://2008.igem.org/Team:Heidelberg/Notebook/Sensing_Group/Notebook/7thweek"