Latest revision as of 20:40, 16 July 2008

Wednesday 4 June

Selim Sheikh:
- Prepared 2 digestion solutions:
  - lambda DNA with XhoI and HindIII
  - lambda DNA with HindIII only
Taylor Stevenson
- Titered the phage stocks obtained from packaging the NEB lambda genome. See packaging manual [1].
- Result-no colonies grew with a 1/200 (phage mixture:cell culture) was plated. Additionally, the more diluted phage mixture showed a titer of approximately 10^5 pfu/uL.

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 =Wednesday 4 June=
 *Selim Sheikh:
-**Prepare 2 digestion solutions:
+**Prepared 2 digestion solutions:
 ***lambda DNA with XhoI and HindIII
 ***lambda DNA with HindIII only
-**Prepare gel electrophoresis of above digests with 1Kb standard
+*Taylor Stevenson
-[[Image:20080604_gel.jpg|100px|left]]
+**Titered the phage stocks obtained from packaging the NEB lambda genome. See packaging manual [https://static.igem.org/mediawiki/2008/6/6a/Packaging_Extract.pdf].
-(from left to right, lane 1: 1Kb, lane 2: lambda with HindIII, lane 3: lambda with XhoI/HindIII)
+**'''Result'''-no colonies grew with a 1/200 (phage mixture:cell culture) was plated.  Additionally, the more diluted phage mixture showed a titer of approximately 10^5 pfu/uL.
+<BR<BR><BR><BR>
+{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
+!align="center"|[[Team:Rice_University|Home]]
+!align="center"|[[Team:Rice_University/Team|The Team]]
+!align="center"|[[Team:Rice_University/Project|The Project]]
+!align="center"|[[Team:Rice_University/Parts|Parts Submitted to the Registry]]
+!align="center"|[[Team:Rice_University/Modeling|Modeling]]
+!align="center"|[[Team:Rice_University/Notebook|Notebook]]
+|}