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Team:Princeton/Experiments
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| - | + | ==Bio-brick Creation== | |
| + | |||
'''Lab Protocols''' | '''Lab Protocols''' | ||
| - | 1. Primer Design | + | {| |
| - | 2. PCR | + | |1. Primer Design |
| - | 3. Run Gel | + | |
| - | 4. | + | 2. [[PCR Amplification]] |
| - | 5. Digestion | + | |
| - | 6. CIP Treatment | + | 3. [[Run Gel/ Gel extraction]] |
| - | 7. PCR Purification | + | |
| - | 8. | + | 4. [[PCR SOEing]] |
| - | 9. Transformation and Plating | + | |
| - | 10. Extract DNA (Miniprep) | + | 5. [[Digestion]] |
| - | 11. Restriction Map/ Digest | + | |
| - | 12. Re-transform with selected plasmid | + | 6. [[CIP Treatment]] |
| - | 13. Extract DNA (Maxiprep or Midiprep) | + | |
| - | 14. Sequence | + | 7. [[PCR Purification]] |
| + | |||
| + | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| | ||
| + | |8. [[Ligate DNA]] | ||
| + | |||
| + | 9. [[Transformation and Plating]] | ||
| + | |||
| + | 10. [[Extract DNA (Miniprep)]] | ||
| + | |||
| + | 11. [[Restriction Map/ Restriction Digest]] | ||
| + | |||
| + | 12. [[Re-transform with selected plasmid]] | ||
| + | |||
| + | 13. [[Extract DNA (Maxiprep or Midiprep)]] | ||
| + | |||
| + | 14. [[Sequence]] | ||
| + | |} | ||
| + | |||
| + | |||
| + | ==Bio-Brick Verification== | ||
| + | |||
| + | '''Cell Culture Process''' | ||
| + | |||
| + | 1. [[Lentivirus Production]] | ||
| + | |||
| + | 2. [[Lentivirus Harvesting]] | ||
| + | |||
| + | 3. [[Infection]] | ||
| + | |||
| + | |||
| + | |||
| + | Also, | ||
| - | + | 1. [[Transfection (nonviral)]] | |
Latest revision as of 07:57, 29 October 2008
PRINCETON IGEM 2008
| Home | Project Overview | Project Details | Experiments | Results | Notebook |
|---|
| Parts Submitted to the Registry | Modeling | The Team | Gallery |
|---|
Bio-brick Creation
Lab Protocols
| 1. Primer Design
4. PCR SOEing 5. Digestion | 8. Ligate DNA
11. Restriction Map/ Restriction Digest 12. Re-transform with selected plasmid 13. Extract DNA (Maxiprep or Midiprep) 14. Sequence |
Bio-Brick Verification
Cell Culture Process
3. Infection
Also,
