Team:Hawaii/Protocols

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Latest revision as of 23:52, 18 July 2008

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List of Protocols

This is a list of tried and true protocols used by our team.

In vitro

E. coli

Synechocystis sp. PCC6803

Triparental conjugation of Synechocystis sp. PCC6803

Media

Solid growth media
Liquid growth media
Antibiotics

BioBrick

[http://openwetware.org/wiki/BioBricks_construction_tutorial BioBrick Construction Tutorial]
[http://openwetware.org/wiki/Silver:_BB_Strategy Silver:BioBrick Strategy]
[http://openwetware.org/wiki/Designing_primers#BioBrick_primers Designing BioBrick Primers]
[http://openwetware.org/wiki/Synthetic_Biology:BioBricks/Part_fabrication BioBrick Part Fabrication]

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

@@ Line 4: / Line 4: @@
 == List of Protocols ==
 This is a list of tried and true protocols used by our team.
+===''In vitro''===
+* [[Team:Hawaii/Protocols/oligo_hybridization|Oligonucleotide hybridization]]
+* [[Team:Hawaii/Procols/DNA_ligation|DNA ligation]]
+* [[DNA restriction digest]]
+* [[Team:Hawaii/Protocols/gel_extraction|Gel extraction]]
+* [[Team:Hawaii/Protocols/ExoSAP|ExoSAP]]
+===''E. coli''===
+* [[Team:Hawaii/Protocols/Cryopreserve_cells|Cryopreserve cells]]
 * [[Team:Hawaii/Protocols/Competent_Cells | Making competent cells]]
 * [[Team:Hawaii/Protocols/Transformation_E_coli|Transformation of '' E. coli'']]
+* [[Team:Hawaii/Protocols/Plasmid_prep|Alkaline lysis / plasmid isolation]]
+* [[Team:Hawaii/Protocols/Colony_PCR|Colony PCR]]
+=== ''Synechocystis'' sp. PCC6803===
 * [[Team:Hawaii/Protocols/Transformation_PCC6803|Triparental conjugation of ''Synechocystis'' sp. PCC6803]]
-* Alkaline lysis / plasmid isolation
-* Solid growth media
+=== Media===
-:* LB
+* [[Team:Hawaii/Protocols/Solid_growth_media|Solid growth media]]
-:* LB + amp<sub>100</sub>
-:* LB + kan<sub>20</sub> + amp <sub>50</sub>
-:* LB + sp<sub>100</sub>
-:* LB + sp<sub>100</sub> + sm<sub>50</sub>
-:* BG-11 + 5% LB
-:* BG-11
-:* BG-11 + sp<sub>2.5</sub> + sm<sub>2.5</sub>
 * Liquid growth media
+* [[Team:Hawaii/Protocols/Antibiotics|Antibiotics]]
+=== BioBrick ===
+* [http://openwetware.org/wiki/BioBricks_construction_tutorial BioBrick Construction Tutorial]
+* [http://openwetware.org/wiki/Silver:_BB_Strategy Silver:BioBrick Strategy]
+* [http://openwetware.org/wiki/Designing_primers#BioBrick_primers Designing BioBrick Primers]
+* [http://openwetware.org/wiki/Synthetic_Biology:BioBricks/Part_fabrication BioBrick Part Fabrication]
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