Team:KULeuven/6 September 2008

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Busy day for a saturday ;)
Busy day for a saturday ;)
* Made some new electrocompetent TOP10 cells, we've got about 32 new aliquots of bacteria happy to be fried by a thousand volts.
* Made some new electrocompetent TOP10 cells, we've got about 32 new aliquots of bacteria happy to be fried by a thousand volts.
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* PCR with prefix-sufix primers on R1052 and the hybrid promoter. Gave some weird results. Will need to be looked at again.
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* PCR with prefix-sufix primers on [http://partsregistry.org/Part:BBa_R1052 R1052] and the hybrid promoter. Gave some weird results. Will need to be looked at again.
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* Made a couple digests: R0040+E0240, C0061+B0015 and C0056+B0015 were cut with ''Eco''RI, E0040 was cut with ''Spe''I and ''Pst''I, and B0015 was cut with ''Xba''I and ''Pst''I. They were put on gel and then purified. The results looked very good.
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* Made a couple digests: [http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_E0240 E0240], [http://partsregistry.org/Part:BBa_C0056 C0056]+[http://partsregistry.org/Part:BBa_B0015 B0015] and [http://partsregistry.org/Part:BBa_C0061 C0061]+[http://partsregistry.org/Part:BBa_B0015 B0015] were cut with ''Eco''RI, E0040 was cut with ''Spe''I and ''Pst''I, and [http://partsregistry.org/Part:BBa_B0015 B0015] was cut with ''Xba''I and ''Pst''I. They were put on gel and then purified. The results looked very good.
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* Also made a digest of K145201, K145205, K145253, K145251 and R0040+J23022+J23109+J23032, but then I forgot to load the Smartladder onto the gel. These digest were set up again (overnight reaction). Anyway, the gel already looked very promising ;-)
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* Also made a digest of [http://partsregistry.org/Part:BBa_K145201 K145201], [http://partsregistry.org/Part:BBa_K145205 K145205], [http://partsregistry.org/Part:BBa_K145253 K145253], [http://partsregistry.org/Part:BBa_K145251 K145251] and [http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23022 J23022]+[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032], but then I forgot to load the Smartladder onto the gel. These digest were set up again (overnight reaction). Anyway, the gel already looked very promising ;-)
* Started some new ligations (mainly to test the new hybrid promoter): R0040+P0353, (R0040+B0032)+(C0062+B0015), (R0011+B0032)+(C0061+B0015) and E0040+B0015.
* Started some new ligations (mainly to test the new hybrid promoter): R0040+P0353, (R0040+B0032)+(C0062+B0015), (R0011+B0032)+(C0061+B0015) and E0040+B0015.
* We set up a PCR to test our ligations using the VF2/VR primers.  
* We set up a PCR to test our ligations using the VF2/VR primers.  
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=== Dry Lab ===
 
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Check our [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=KULeuven KULeuven sandbox] over at the Registry to see some nice Building/Available/Working statusses 'n stuff
 
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==== Modeling ====
 
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==== Wiki ====
 
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== Remarks ==
 
== Comic of the day ==
== Comic of the day ==
<div class="center">http://www.phdcomics.com/comics/archive/phd090308s.gif</div>
<div class="center">http://www.phdcomics.com/comics/archive/phd090308s.gif</div>

Latest revision as of 01:41, 30 October 2008

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Lab Work

Wet Lab

Busy day for a saturday ;)

  • Made some new electrocompetent TOP10 cells, we've got about 32 new aliquots of bacteria happy to be fried by a thousand volts.
  • PCR with prefix-sufix primers on [http://partsregistry.org/Part:BBa_R1052 R1052] and the hybrid promoter. Gave some weird results. Will need to be looked at again.
  • Made a couple digests: [http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_E0240 E0240], [http://partsregistry.org/Part:BBa_C0056 C0056]+[http://partsregistry.org/Part:BBa_B0015 B0015] and [http://partsregistry.org/Part:BBa_C0061 C0061]+[http://partsregistry.org/Part:BBa_B0015 B0015] were cut with EcoRI, E0040 was cut with SpeI and PstI, and [http://partsregistry.org/Part:BBa_B0015 B0015] was cut with XbaI and PstI. They were put on gel and then purified. The results looked very good.
  • Also made a digest of [http://partsregistry.org/Part:BBa_K145201 K145201], [http://partsregistry.org/Part:BBa_K145205 K145205], [http://partsregistry.org/Part:BBa_K145253 K145253], [http://partsregistry.org/Part:BBa_K145251 K145251] and [http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23022 J23022]+[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032], but then I forgot to load the Smartladder onto the gel. These digest were set up again (overnight reaction). Anyway, the gel already looked very promising ;-)
  • Started some new ligations (mainly to test the new hybrid promoter): R0040+P0353, (R0040+B0032)+(C0062+B0015), (R0011+B0032)+(C0061+B0015) and E0040+B0015.
  • We set up a PCR to test our ligations using the VF2/VR primers.

Comic of the day

http://www.phdcomics.com/comics/archive/phd090308s.gif
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