Team:ESBS-Strasbourg/Protocols

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=Protocols=
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{|
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==E.Coli==
==E.Coli==
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*'''Preparation of competent cells (Mariels/Barbaras Protocol)''' [[Team:ESBS-Strasbourg/Competent Bacteria | Competent Bacteria]] <br>
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*[[Team:ESBS-Strasbourg/Competent Bacteria | Preparation of competent cells (Barbara's Protocol)]] <br>
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*[http://openwetware.org/wiki/TOP10_chemically_competent_cells Preparation of TOP10 chemically competent cells (iGEM)] <br>
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*[[Team:ESBS-Strasbourg/Transformation Barbara|Transformation of competent cells (Barbara's Protocol)]]<br>
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*[[Team:ESBS-Strasbourg/Transformation Heat shock|Heat shock transformation]]<br>
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*'''Preparation of TOP10 chemically competent cells''' [http://openwetware.org/wiki/TOP10_chemically_competent_cells Igem Standard Protocol] <br>
 
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*'''Transformation of competent cells (Mariels/Barbaras Protocol)''' [[Team:ESBS-Strasbourg/Transformation Barbara|Transformation]]<br>
 
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*'''Transformation of competent cells with heat shock''' [[Team:ESBS-Strasbourg/Transformation Heat shock|Transformation HS]]<br>
 
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==Cloning==
==Cloning==
*[[Team:ESBS-Strasbourg/PCR|PCR]] <br>
*[[Team:ESBS-Strasbourg/PCR|PCR]] <br>
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*[[Team:ESBS-Strasbourg/Ligation|Ligation]] <br>
*[[Team:ESBS-Strasbourg/Ligation|Ligation]] <br>
*[[Team:ESBS-Strasbourg/Concentrations Measurements|Concentrations Measurements]] <br>
*[[Team:ESBS-Strasbourg/Concentrations Measurements|Concentrations Measurements]] <br>
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==Solutions and buffers==
==Solutions and buffers==
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*'''SOB''' for the preparation of competent cells [http://openwetware.org/wiki/SOB SOB] <br>
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*[http://openwetware.org/wiki/SOB SOB for the preparation of competent cells] <br>
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*'''SOC''' for bacterial transformation purposes [http://openwetware.org/wiki/SOC SOC] <br>
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*[http://openwetware.org/wiki/SOC SOC for bacterial transformation purposes] <br>
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*'''TENS''' for the do it yourself MiniPrep [[Team:ESBS-Strasbourg/TENS|TENS]] <br>
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*[[Team:ESBS-Strasbourg/TENS|TENS for the do it yourself MiniPrep]] <br>
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Latest revision as of 00:13, 29 October 2008


Protocols


E.Coli

Cloning

Solutions and buffers

  • [http://openwetware.org/wiki/SOB SOB for the preparation of competent cells]
  • [http://openwetware.org/wiki/SOC SOC for bacterial transformation purposes]
  • TENS for the do it yourself MiniPrep



Further source for protocols: [http://www.protocol-online.org/ Protocol-online.org]


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