Team:Waterloo/Notebook/Oligos

From 2008.igem.org

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{| width="100%" border="1" cellpadding="2"
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!width="500"|Description
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! Description !! Oligo Name !! Sequence !! Length
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!width="500"|Oligo Name
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!width="500"|Sequence
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!width="500"|Length
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|BioBrick sequencing/colPCR primer (fwd)  || iGEMvf2 || TGCCACCTGACGTCTAAGAA || 20
|BioBrick sequencing/colPCR primer (fwd)  || iGEMvf2 || TGCCACCTGACGTCTAAGAA || 20
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|Primer for {BioBrick Prefix}-{B0034} (rev; for use with iGEMvf2)|| iGEMpre+rbs || TTTCTCCTCTTTCTCTAGATGCGG || 24
|Primer for {BioBrick Prefix}-{B0034} (rev; for use with iGEMvf2)|| iGEMpre+rbs || TTTCTCCTCTTTCTCTAGATGCGG || 24
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|Gene 5 ORF from T7 phage (fwd)  || T7gene5-F || CAATGGAATTCGCGGCCGCTTCTAGATGATCGTTTCTGACATCGAA || 46
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|Primer for {BioBrick Prefix}-{B0034} (fwd; for use with iGEMvr)  || PrefixRBS-F || GGCCGCTTCTAGAGAAAGAGG || 21
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|Gene 5 ORF from T7 phage (rev)  || T7gene5-R || CAATTTCTGCAGCGGCCGCTACTAGTATCAGTGGCAAATCGCCCAATTAGG || 51
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|Gene 5 ORF from T7 phage (fwd; Prefix adapter)  || T7gene5-F || CAATGGAATTCGCGGCCGCTTCTAGATGATCGTTTCTGACATCGAA || 46
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|Gene 6 ORF from T7 phage (fwd)  || T7gene6-F || CAATGGAATTCGCGGCCGCTTCTAGATGGCACTTCTTGACCTTAAA || 46
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|Gene 5 ORF from T7 phage (rev; Suffix adapter)  || T7gene5-R || CAATTTCTGCAGCGGCCGCTACTAGTATCAGTGGCAAATCGCCCAATTAGG || 51
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|Gene 6 ORF from T7 phage (rev)  || T7gene6-R || CAATTTCTGCAGCGGCCGCTACTAGTACTACGGTCTCCACAGG || 43
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|Gene 6 ORF from T7 phage (fwd; Prefix adapter)  || T7gene6-F || CAATGGAATTCGCGGCCGCTTCTAGATGGCACTTCTTGACCTTAAA || 46
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|  || PrefixRBS-F || GGCCGCTTCTAGAGAAAGAGG || 21
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|Gene 6 ORF from T7 phage (rev; Suffix adapter) || T7gene6-R || CAATTTCTGCAGCGGCCGCTACTAGTACTACGGTCTCCACAGG || 43
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|  || PmeIR-F || ATCCGGAATTCGCGGCGCTTTCTAGATGACAACAAACTCCCCCTCAGACG || 50
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|PmeIR ORF (fwd) || PmeIR-F || ATCCGGAATTCGCGGCGCTTTCTAGATGACAACAAACTCCCCCTCAGACG || 50
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|  || PmelR-R || CGCAAAACTGCAGCGGCCGCTACTAGTATCAAACCGTACTTCGCCCCCGTTG || 52
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|PmeIR ORF (rev) || PmelR-R || CGCAAAACTGCAGCGGCCGCTACTAGTATCAAACCGTACTTCGCCCCCGTTG || 52
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|  || phbA-F || GAATTCGCGGCCGCTTCTAGATGAGCAATCCCTCGATCG || 39
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|pRecA oligo (sense strand; produces BioBrick-compatible EcoRI/SpeI overhangs when annealed to recAR) || recAF || AATTCGCGGCCGCTTCTAGAGTACAAAACACTTGATACTGTATATATATACAGTATAATTGCTTCAACATA || 71
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|  || phbA-R || CTGCAGCGGCCGCTACTAGTATTACAGGCGTTCCACGCACATGG || 44
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|pRecA oligo (antisense strand; produces BioBrick-compatible EcoRI/SpeI overhangs when annealed to recAR) || recAR || CTAGTATGTTGAAGCAATTATACTGTATATATATACAGTATCAAGTGTTTTGTACTCTAGAAGCGGCCGCG || 71
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|  || phbB-F || GAATTCGCGGCCGCTTCTAGATGAGCAGGGTAGCACTGG || 39
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|{B0034}-{T7gene6 ORF} (fwd; for adding {Prefix-RBS} onto the gene 6 ORF by PCR) || RBST7g6-F || GAATTCCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGGCACTTCTTGACCTTAAAC || 61
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|  || phbB-R || CTGCAGCGGCCGCTACTAGTATCAGGCGAAGTACTGGCCGC || 41
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|T7gene6 ORF (rev) || T7gs-R || CTGCAGCGGCCGCTACTAGTATTATTACGGTCTCCACAGGTAAATCTC || 48
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|  || phbC-F || GAATTCGCGGCCGCTTCTAGATGACAGCAGAGAAGGCTGAGG || 42
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|T7gene6 ORF rev; for joining to PmeI with crossover PCR || T7g6-RXO-RBS || CTAGTATTTCTCCTCTTTCTCTAGTATTATTACGGTCTCCACAGGTAAATCTC || 53
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|  || phbC-R || CTGCAGCGGCCGCTACTAGTATCAGGCGCGCACGCGCACGTAGG || 44
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|PmeI ORF (fwd; for adding prefix-RBS) || RBSpmeI-F || GAATTCCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGACAACAAACTCCCCCTCAG || 61
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| || recAF || AATTCGCGGCCGCTTCTAGAGTACAAAACACTTGATACTGTATATATATACAGTATAATTGCTTCAACATA || 71
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|PmeI ORF (fwd; for adding prefix-RBS and joining to T7gene6 by crossover PCR) || RBSpmeI-FXO || TACTAGAGAAAGAGGAGAAATACTAGATGACAACAAACTCCCCCTCAG || 48
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|  || recAR || CTAGTATGTTGAAGCAATTATACTGTATATATATACAGTATCAAGTGTTTTGTACTCTAGAAGCGGCCGCG || 71
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|PmeI ORF (rev; TAATAA stop codon-suffix) || pmeI-R || CTGCAGCGGCCGCTACTAGTATTATTAAACCGTACTTCGCCCCCG || 45
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| || RBST7g6-F || GAATTCCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGGCACTTCTTGACCTTAAAC || 61
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|Primer for part R0011 (Plac-PL hybrid promoter; fwd) || R0011-F || GAATTCCGGCCGCTTCTAGAGAATTGTGAGCGGATAAC || 38
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| || T7gs-R || CTGCAGCGGCCGCTACTAGTATTATTACGGTCTCCACAGGTAAATCTC || 48
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|Primer for part R0011 (Plac-PL hybrid promoter; rev for joining to ORFs with RBS by crossover PCR)|| R0011RBS-RXO || CTAGTATTTCTCCTCTTTCTCTAGTATGTGCTCAGTATCTTGTTATC || 47
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| || T7g6-RXO-RBS || CTAGTATTTCTCCTCTTTCTCTAGTATTATTACGGTCTCCACAGGTAAATCTC || 53
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|CI ORF (fwd; prefix-RBS) || RBSC0051-F || GAATTCCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGAGCACAAAAAAGAAACC || 59
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| || RBSpmeI-F || GAATTCCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGACAACAAACTCCCCCTCAG || 61
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|CI ORF (fwd; prefix-RBS for joining to R0011 promoter by crossover PCR) || RBSC0051-FXO || TACTAGAGAAAGAGGAGAAATACTAGATGAGCACAAAAAAGAAACC || 46
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|  || RBSpmeI-FXO || TACTAGAGAAAGAGGAGAAATACTAGATGACAACAAACTCCCCCTCAG || 48
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|CI ORF (rev; suffix adapter) || C0051-R || CTGCAGCGGCCGCTACTAGTATTATTAAGCTACTAAAGCG || 40
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|  || pmeI-R || CTGCAGCGGCCGCTACTAGTATTATTAAACCGTACTTCGCCCCCG || 45
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|CI ORF (rev; for joining to transcriptional terminator (b0015) by crossover PCR) || C0051-RXO || GATGCCTGGCTCTAGTATTATTAAGCTACTAAAGCG || 36
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|  || R0011-F || GAATTCCGGCCGCTTCTAGAGAATTGTGAGCGGATAAC || 38
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|Transcriptional terminator (B0015; fwd; for joining to ORFs by crossover PCR) || B0015-FXO || TAATAATACTAGAGCCAGGCATCAAATAAAACG || 33
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|  || R0011RBS-RXO || CTAGTATTTCTCCTCTTTCTCTAGTATGTGCTCAGTATCTTGTTATC || 47
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|Transcriptional terminator (B0015; rev) || B0015-R || CTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCC || 39
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|  || RBSC0051-F || GAATTCCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGAGCACAAAAAAGAAACC || 59
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|RFP ORF (fwd; prefix-RBS) || RFP-RBS-F || GAATTCCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGGCTTCCTCCGAAGACG || 58
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| || RBSC0051-FXO || TACTAGAGAAAGAGGAGAAATACTAGATGAGCACAAAAAAGAAACC || 46
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|RFP ORF (fwd; prefix-RBS for joining to transcriptional terminator by crossover PCR ) || RFP-RXO || GATGCCTGGCTCTAGTATTATTAAGCACCGGTGG || 34
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| || C0051-R || CTGCAGCGGCCGCTACTAGTATTATTAAGCTACTAAAGCG || 40
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|Plac (r0011; fwd; for adding lac promoter and RBS to ORFs by PCR)  || Plac-RBS-F || GAATTCGCGGCCGCTTCTAGAGAATTGTGAGCGGATAACAATTGACATTGTGAGCGGATAACAAGATACTGAGCAC ATACTAGAGAAAGAGGAGAAATACTAG || 103
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|  || C0051-RXO || GATGCCTGGCTCTAGTATTATTAAGCTACTAAAGCG || 36
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|  || B0015-FXO || TAATAATACTAGAGCCAGGCATCAAATAAAACG || 33
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|  || B0015-R || CTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCC || 39
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|  || RFP-RBS-F || GAATTCCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGGCTTCCTCCGAAGACG || 58
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|  || RFP-RXO || GATGCCTGGCTCTAGTATTATTAAGCACCGGTGG || 34
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|  || Plac-RBS-F || GAATTCGCGGCCGCTTCTAGAGAATTGTGAGCGGATAACAATTGACATTGTGAGCGGATAACAAGATACTGAGCACATACTAGAGAAAGAGGAGAAATACTAG || 103
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Latest revision as of 03:57, 30 October 2008

Home The Team The Project Parts Submitted to the Registry Modeling Notebook Sponsors
Description Oligo Name Sequence Length
BioBrick sequencing/colPCR primer (fwd) iGEMvf2 TGCCACCTGACGTCTAAGAA 20
BioBrick sequencing/colPCR primer (rev) iGEMvr ATTACCGCCTTTGAGTGAGC 20
Primer for Lac promoter (fwd) PLacF TTATGCTTCCGGCTCGTATG 20
Primer for {BioBrick Prefix}-{B0034} (rev; for use with iGEMvf2) iGEMpre+rbs TTTCTCCTCTTTCTCTAGATGCGG 24
Primer for {BioBrick Prefix}-{B0034} (fwd; for use with iGEMvr) PrefixRBS-F GGCCGCTTCTAGAGAAAGAGG 21
Gene 5 ORF from T7 phage (fwd; Prefix adapter) T7gene5-F CAATGGAATTCGCGGCCGCTTCTAGATGATCGTTTCTGACATCGAA 46
Gene 5 ORF from T7 phage (rev; Suffix adapter) T7gene5-R CAATTTCTGCAGCGGCCGCTACTAGTATCAGTGGCAAATCGCCCAATTAGG 51
Gene 6 ORF from T7 phage (fwd; Prefix adapter) T7gene6-F CAATGGAATTCGCGGCCGCTTCTAGATGGCACTTCTTGACCTTAAA 46
Gene 6 ORF from T7 phage (rev; Suffix adapter) T7gene6-R CAATTTCTGCAGCGGCCGCTACTAGTACTACGGTCTCCACAGG 43
PmeIR ORF (fwd) PmeIR-F ATCCGGAATTCGCGGCGCTTTCTAGATGACAACAAACTCCCCCTCAGACG 50
PmeIR ORF (rev) PmelR-R CGCAAAACTGCAGCGGCCGCTACTAGTATCAAACCGTACTTCGCCCCCGTTG 52
pRecA oligo (sense strand; produces BioBrick-compatible EcoRI/SpeI overhangs when annealed to recAR) recAF AATTCGCGGCCGCTTCTAGAGTACAAAACACTTGATACTGTATATATATACAGTATAATTGCTTCAACATA 71
pRecA oligo (antisense strand; produces BioBrick-compatible EcoRI/SpeI overhangs when annealed to recAR) recAR CTAGTATGTTGAAGCAATTATACTGTATATATATACAGTATCAAGTGTTTTGTACTCTAGAAGCGGCCGCG 71
{B0034}-{T7gene6 ORF} (fwd; for adding {Prefix-RBS} onto the gene 6 ORF by PCR) RBST7g6-F GAATTCCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGGCACTTCTTGACCTTAAAC 61
T7gene6 ORF (rev) T7gs-R CTGCAGCGGCCGCTACTAGTATTATTACGGTCTCCACAGGTAAATCTC 48
T7gene6 ORF rev; for joining to PmeI with crossover PCR T7g6-RXO-RBS CTAGTATTTCTCCTCTTTCTCTAGTATTATTACGGTCTCCACAGGTAAATCTC 53
PmeI ORF (fwd; for adding prefix-RBS) RBSpmeI-F GAATTCCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGACAACAAACTCCCCCTCAG 61
PmeI ORF (fwd; for adding prefix-RBS and joining to T7gene6 by crossover PCR) RBSpmeI-FXO TACTAGAGAAAGAGGAGAAATACTAGATGACAACAAACTCCCCCTCAG 48
PmeI ORF (rev; TAATAA stop codon-suffix) pmeI-R CTGCAGCGGCCGCTACTAGTATTATTAAACCGTACTTCGCCCCCG 45
Primer for part R0011 (Plac-PL hybrid promoter; fwd) R0011-F GAATTCCGGCCGCTTCTAGAGAATTGTGAGCGGATAAC 38
Primer for part R0011 (Plac-PL hybrid promoter; rev for joining to ORFs with RBS by crossover PCR) R0011RBS-RXO CTAGTATTTCTCCTCTTTCTCTAGTATGTGCTCAGTATCTTGTTATC 47
CI ORF (fwd; prefix-RBS) RBSC0051-F GAATTCCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGAGCACAAAAAAGAAACC 59
CI ORF (fwd; prefix-RBS for joining to R0011 promoter by crossover PCR) RBSC0051-FXO TACTAGAGAAAGAGGAGAAATACTAGATGAGCACAAAAAAGAAACC 46
CI ORF (rev; suffix adapter) C0051-R CTGCAGCGGCCGCTACTAGTATTATTAAGCTACTAAAGCG 40
CI ORF (rev; for joining to transcriptional terminator (b0015) by crossover PCR) C0051-RXO GATGCCTGGCTCTAGTATTATTAAGCTACTAAAGCG 36
Transcriptional terminator (B0015; fwd; for joining to ORFs by crossover PCR) B0015-FXO TAATAATACTAGAGCCAGGCATCAAATAAAACG 33
Transcriptional terminator (B0015; rev) B0015-R CTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCC 39
RFP ORF (fwd; prefix-RBS) RFP-RBS-F GAATTCCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGGCTTCCTCCGAAGACG 58
RFP ORF (fwd; prefix-RBS for joining to transcriptional terminator by crossover PCR ) RFP-RXO GATGCCTGGCTCTAGTATTATTAAGCACCGGTGG 34
Plac (r0011; fwd; for adding lac promoter and RBS to ORFs by PCR) Plac-RBS-F GAATTCGCGGCCGCTTCTAGAGAATTGTGAGCGGATAACAATTGACATTGTGAGCGGATAACAAGATACTGAGCAC ATACTAGAGAAAGAGGAGAAATACTAG 103


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