Latest revision as of 06:46, 27 June 2008

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Things we did today

Margaret, Krystle

Grace

Grace

Restreaked transformed PCC6803 on new BG-11+sm_2.5+sp_2.5 plates for purification

Grace, Krystle

Planned timeline (still in works) for secretion project
Will convert pRL1383a into a BioBrick format plasmid so testing in PCC6803 can begin ASAP

Margaret

I am updating the wiki with the details of this experiment: the how to and the what went wrong.

Oooh, look at the pretty colors - Krystle, refering to the agarose gel

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

@@ Line 8: / Line 8: @@
 :*Ran gel but DNA did not migrate.
 :*Restriction digest of prep with EcoRI and ran gel.
+===Cell density count===
+:<strong>Grace</strong>
+:* Completed [[Team:Hawaii/PCC6803_cell_density|cell density count experiment]]
+=== Purified triparental conjugants===
+:<strong>Grace</strong>
+:* Restreaked transformed PCC6803 on new BG-11+sm<sub>2.5</sub>+sp<sub>2.5</sub> plates for purification
 == Drylab Work ==
-===Design of Plasmid for Secretion Project===
+===Timeline for Secretion Project===
 :<strong> Grace, Krystle </strong>
-:* Summary of task and what was done. Link to experiment for detailed notes if necessary.
+:* Planned timeline (still in works) for secretion project
-:* e.g. read through papers, worked on proposal, etc.
+:* Will convert pRL1383a into a BioBrick format plasmid so testing in PCC6803 can begin ASAP
 ===Large Scale Plasmid Prep===
@@ Line 24: / Line 34: @@
 = Quote of the Day =
-<blockquote>''History is the only laboratory we have in which to test the consequences of thought.'' - Étienne Gilson</blockquote>
+<blockquote>''Oooh, look at the pretty colors'' - Krystle, refering to the agarose gel</blockquote>
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