Team:Freiburg

From 2008.igem.org

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{{Freiburg2008_Start|
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<font face="Arial Rounded MT Bold" style="color:#010369">_welcome!</font></div><br><br>
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[[image:Freiburg2008_extracts.png|800px]]<br>
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The first German team ever to participate in iGEM is back again, providing you with an excellent set of [[Team:Freiburg/Parts|fusion proteins]] for modular use as [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=Freiburg BioBricks]. This year we are crafting a [[Team:Freiburg/Project|synthetic receptor kit]] "made in Black Forest", using [[DNA-Origami]] as programmable input device and the complementation of split-fluorophores and -enzymes as readable output.<br>
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An [https://2007.igem.org/Freiburg07/report_fusion_parts extension of the standard] BioBrick Pre- and Suffix, developed by iGEM-Team Freiburg 2007, allows in-frame-cloning of proteins without generation of a stop-codon at the "scar", so that we can fuse practically any number of proteins and/or peptides - as you can see in our [[Team:Freiburg Cloning Strategy|cloning strategy]] page. Of course, we want to share this possibility with future iGEM-Teams and have added a new, fully compatible [http://partsregistry.org/Part:BBa_K157000 plasmid] to the registry.
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[[Image:Freiburg2008_Bilderleiste.jpg|800px]]
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==  Project Abstract ==
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This is a template page. READ THESE INSTRUCTIONS.
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[[Image:Freiburg08_MSRS_Schema.png|thumb|270px|left|Schematical overview of the system]]
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You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
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'''Modular Synthetic Receptor System:'''<br>
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You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, and a lab notebook.  PLEASE keep all of your pages within your Team:Example namespace. 
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Signaling through membranes is a characteristic of life. Transmembrane proteins control proliferation, differentiation, and cellular response and are key for the formation of multicellular organisms. Controlling such proteins enables modifying cellular behavior and ultimately programming cells at will. The complex rules for transmembrane signaling often require engagement of several proteins in a fine-tuned spatial and temporal manner.
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To tap possibilities of transmembrane programming, the Freiburg 2008 iGEM team provides an extensible system comprising an external framework with spatial resolution, a concept for modifying natural receptors, and a modular set of fusion-Biobricks for the construction of synthetic receptors. Spatial resolution in the nanometer scale is provided by DNA-Origami modified with distinct patterns and combinations of ligands. Receptors are decoupled from their natural ligands by fusion with artificial binding domains. The Biobrick collection contains signal sequences, binding domains, transmembrane domains, and effector domains featuring split enzymes and split fluorescent proteins for immediate readout.<br>
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For details see the [[Team:Freiburg/Project|Project Report :]]<br>
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*[[Team:Freiburg/Modeling|Modeling]]<br>
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*[[Team:Freiburg/3D-Modeling|3D-Modeling]]<br>
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*[[DNA-Origami|DNA-Origami]]<br>
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*[[Team:Freiburg_Cloning Strategy|Cloning Strategy]]<br>
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*[[Team:Freiburg_Transfection and Synthetic Receptor|Transfection and Synthetic Receptor Activation]]<br>
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*[[Team:Freiburg_Calcium Imaging|Cell Stability, Ca<sup>2+</sup> Signaling and DNA-Origami-Binding]]
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==  ==
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[[Team:Freiburg_Sponsoring|Supporters:]]<br><br>
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[[Image:Freiburg2008_Sponsorenleiste2.png|830px]]
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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|align="center"|[[Team:Freiburg | Team Example 2]]
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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!align="center"|[[Team:Freiburg|Home]]
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!align="center"|[[Team:Freiburg/Team|The Team]]
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!align="center"|[[Team:Freiburg/Project|The Project]]
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!align="center"|[[Team:Freiburg/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Freiburg/Modeling|Modeling]]
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!align="center"|[[Team:Freiburg/Notebook|Notebook]]
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(''Or you can choose different headings.  But you must have a team page, a project page, and a notebook page.'')
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Latest revision as of 15:47, 15 November 2008


Freiburg2008 Header.png



Home

The Team

Project Report

Parts

Modeling

Notebook

Safety

CoLABoration
_welcome!


Freiburg2008 extracts.png

Freiburg2008 Fab on Origami animated.gif

The first German team ever to participate in iGEM is back again, providing you with an excellent set of fusion proteins for modular use as [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=Freiburg BioBricks]. This year we are crafting a synthetic receptor kit "made in Black Forest", using DNA-Origami as programmable input device and the complementation of split-fluorophores and -enzymes as readable output.
An extension of the standard BioBrick Pre- and Suffix, developed by iGEM-Team Freiburg 2007, allows in-frame-cloning of proteins without generation of a stop-codon at the "scar", so that we can fuse practically any number of proteins and/or peptides - as you can see in our cloning strategy page. Of course, we want to share this possibility with future iGEM-Teams and have added a new, fully compatible [http://partsregistry.org/Part:BBa_K157000 plasmid] to the registry.

Freiburg2008 Bilderleiste.jpg

Project Abstract


Schematical overview of the system

Modular Synthetic Receptor System:

Signaling through membranes is a characteristic of life. Transmembrane proteins control proliferation, differentiation, and cellular response and are key for the formation of multicellular organisms. Controlling such proteins enables modifying cellular behavior and ultimately programming cells at will. The complex rules for transmembrane signaling often require engagement of several proteins in a fine-tuned spatial and temporal manner.

To tap possibilities of transmembrane programming, the Freiburg 2008 iGEM team provides an extensible system comprising an external framework with spatial resolution, a concept for modifying natural receptors, and a modular set of fusion-Biobricks for the construction of synthetic receptors. Spatial resolution in the nanometer scale is provided by DNA-Origami modified with distinct patterns and combinations of ligands. Receptors are decoupled from their natural ligands by fusion with artificial binding domains. The Biobrick collection contains signal sequences, binding domains, transmembrane domains, and effector domains featuring split enzymes and split fluorescent proteins for immediate readout.
For details see the Project Report :


Supporters:

Freiburg2008 Sponsorenleiste2.png

Freiburg08 FT3.png

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