Team:Paris/Construction
From 2008.igem.org
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|- style="background: #dddddd;" | |- style="background: #dddddd;" | ||
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFliL (+RBS WT) | | style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFliL (+RBS WT) | ||
- | |align=| | + | |align=| Natural promoter with the wild type rbs of its associated gene in E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC and FliA. |
|align="center"| [[Team:Paris/October 10|October 10]] | |align="center"| [[Team:Paris/October 10|October 10]] | ||
|- style="background: #dddddd;" | |- style="background: #dddddd;" | ||
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlgA (+RBS WT) | | style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlgA (+RBS WT) | ||
- | |align=| | + | |align=| Natural promoter with the wild type rbs of its associated gene in E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC and FliA.. |
|align="center"| [[Team:Paris/October 10|October 10]] | |align="center"| [[Team:Paris/October 10|October 10]] | ||
|- style="background: #dddddd;" | |- style="background: #dddddd;" | ||
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlgB (+RBS WT) | | style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlgB (+RBS WT) | ||
- | |align=| | + | |align=| Natural promoter with the wild type rbs of its associated gene in E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC and FliA. |
|align="center"| [[Team:Paris/October 10|October 10]] | |align="center"| [[Team:Paris/October 10|October 10]] | ||
|- style="background: #dddddd;" | |- style="background: #dddddd;" | ||
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlhB (+RBS WT) | | style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlhB (+RBS WT) | ||
- | |align=| | + | |align=| Natural promoter with the wild type rbs of its associated gene in E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC and FliA. |
|align="center"| [[Team:Paris/October 10|October 10]] | |align="center"| [[Team:Paris/October 10|October 10]] | ||
|- style="background: #dddddd;" | |- style="background: #dddddd;" | ||
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>EnvZ* | | style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>EnvZ* | ||
- | |align=| | + | |align=| EnvZ is a membrane protein involved into the osmotic response and is interacting with OmpR that will bind the promoter of FlhDC ad will repress it. EnvZ* is a mutated form of the coding sequence, giving a phenotype where pFlhDC is completely repressed. EnvZ has been extracted by PVR for the correct mutant strain. |
|align="center"| [[Team:Paris/September 04|September 04]] | |align="center"| [[Team:Paris/September 04|September 04]] | ||
|- style="background: #dddddd;" | |- style="background: #dddddd;" | ||
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>FliA | | style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>FliA | ||
- | |align=| | + | |align=| FliA is a sigma factor involved into the regulation of very various genes, the promoter used in the FIFO were selected to be higly responding to FliA. In order to have the FliA gene possibly used as biobrick, we mutated 2 sites (EcoRI and PstI) inside the FliA sequence by assemby PCR. |
|align="center"| [[Team:Paris/September 04|September 04]] | |align="center"| [[Team:Paris/September 04|September 04]] | ||
|- style="background: #dddddd;" | |- style="background: #dddddd;" | ||
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>FlhDC | | style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>FlhDC | ||
- | |align=| | + | |align=| FlhDC is the main regulator of the flagella genetic system in E. coli. FlhDC regulates all the promoter of the FIFO. |
|align="center"| [[Team:Paris/August 13|August 13]] | |align="center"| [[Team:Paris/August 13|August 13]] | ||
|} | |} |
Revision as of 03:20, 30 October 2008
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