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Alberta NINT/27 May 2008
From 2008.igem.org
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Run on 2% agarose gel. ~3 kbp fragment expected. | Run on 2% agarose gel. ~3 kbp fragment expected. | ||
Ligated K102000 with E0433 and K102000 with null_output. | Ligated K102000 with E0433 and K102000 with null_output. | ||
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lab work (JD): | lab work (JD): | ||
Revision as of 20:07, 8 July 2008
lab work (SD):
DNA digest of K102000 with SpeI + PstI. Incubated at 37 C for 1 hour.
Run on 2% agarose gel. ~3 kbp fragment expected.
Ligated K102000 with E0433 and K102000 with null_output.
lab work (JD):
Recovered Part BBA_R011 from iGEM package
Transformed XL1-B cells and plated on LB+Amp100 plates.
Created 2 O/Ns as well.
lab work (WM):
Prepare BBa_K102010 fragments for ligation.
K102010 is AraC::PromBad::TA0In
AraC::PromBad comes is in BBa_I0500/SpeI+PstI (5.6kb fragment, including plasmid)
TA0In (200bp) was synthesized commercially and placed in a vector.
Gel-purify BBa_I0500/SpeI+PstI and TA0In?XbaI+PstI fragments from 2% agarose gel.
