Team:Paris/Notebook/Oligo

From 2008.igem.org

(Difference between revisions)
(→Designed Oligos)
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{{Paris/Menu}}
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|width=5%| Tm(°C)
|width=5%| Tm(°C)
|width=45%| Comments
|width=45%| Comments
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|- style="background: #dddddd;"   
| style="background: #D4E2EF;" |O100
| style="background: #D4E2EF;" |O100
| FlgA-F
| FlgA-F
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|- style="background: #dddddd;"
| style="background: #D4E2EF;" | O101
| style="background: #D4E2EF;" | O101
| FlgA-R
| FlgA-R
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|- style="background: #dddddd;"
| style="background: #D4E2EF;" | O102
| style="background: #D4E2EF;" | O102
| FlgB-F
| FlgB-F
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|- style="background: #dddddd;"
| style="background: #D4E2EF;" | O103
| style="background: #D4E2EF;" | O103
| FlgB-R
| FlgB-R
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|- style="background: #cccccc;"
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|- style="background: #dddddd;"
| style="background: #D4E2EF;" | O104
| style="background: #D4E2EF;" | O104
| FlgK-F
| FlgK-F
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|- style="background: #cccccc;"
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|- style="background: #dddddd;"
| style="background: #D4E2EF;" | O105
| style="background: #D4E2EF;" | O105
| FlgK-R
| FlgK-R
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|- style="background: #cccccc;"
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|- style="background: #dddddd;"
| style="background: #D4E2EF;" | O106
| style="background: #D4E2EF;" | O106
| FlgM-F
| FlgM-F
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|- style="background: #cccccc;"
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|- style="background: #dddddd;"
| style="background: #D4E2EF;" | O107
| style="background: #D4E2EF;" | O107
| FlgM-R
| FlgM-R
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|- style="background: #cccccc;"
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|- style="background: #dddddd;"
| style="background: #D4E2EF;" | O108
| style="background: #D4E2EF;" | O108
| FlhB-F
| FlhB-F
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|- style="background: #cccccc;"
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|- style="background: #dddddd;"
| style="background: #D4E2EF;" | O109
| style="background: #D4E2EF;" | O109
| FlhB-R
| FlhB-R
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|- style="background: #cccccc;"
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|- style="background: #dddddd;"
| style="background: #D4E2EF;" | O110
| style="background: #D4E2EF;" | O110
| FlhDC-Uri-F
| FlhDC-Uri-F
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|Don't amplify the both OmpR binding site
|Don't amplify the both OmpR binding site
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|- style="background: #cccccc;"
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|- style="background: #dddddd;"
| style="background: #D4E2EF;" | O111
| style="background: #D4E2EF;" | O111
| FlhDC-Total-F
| FlhDC-Total-F
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|Amplify the both OmpR binding site
|Amplify the both OmpR binding site
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|- style="background: #cccccc;"
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|- style="background: #dddddd;"
| style="background: #D4E2EF;" | O112
| style="background: #D4E2EF;" | O112
| FlhDC(rbs)-R
| FlhDC(rbs)-R
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|Amplify the natural rbs of FlhD
|Amplify the natural rbs of FlhD
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|- style="background: #cccccc;"
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|- style="background: #dddddd;"
| style="background: #D4E2EF;" | O113
| style="background: #D4E2EF;" | O113
| FlhDC(nu)-R
| FlhDC(nu)-R
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|Don't amplify the natural rbs of FlhD (only promoter)
|Don't amplify the natural rbs of FlhD (only promoter)
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|- style="background: #cccccc;"
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|- style="background: #dddddd;"
| style="background: #D4E2EF;" | O114
| style="background: #D4E2EF;" | O114
| FliA-PCR1-F
| FliA-PCR1-F
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|- style="background: #dddddd;"
| style="background: #D4E2EF;" | O115
| style="background: #D4E2EF;" | O115
| FliA-PCR1-R
| FliA-PCR1-R
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|- style="background: #cccccc;"
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|- style="background: #dddddd;"
| style="background: #D4E2EF;" | O116
| style="background: #D4E2EF;" | O116
| FliA-PCR2-F
| FliA-PCR2-F
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|- style="background: #dddddd;"
| style="background: #D4E2EF;" | O117
| style="background: #D4E2EF;" | O117
| FliA-PCR2-R
| FliA-PCR2-R
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|- style="background: #cccccc;"
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|- style="background: #dddddd;"
| style="background: #D4E2EF;" | O118
| style="background: #D4E2EF;" | O118
| FliC-F
| FliC-F
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|- style="background: #cccccc;"
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|- style="background: #dddddd;"
| style="background: #D4E2EF;" | O119
| style="background: #D4E2EF;" | O119
| FliC-R
| FliC-R
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|- style="background: #dddddd;"
| style="background: #D4E2EF;" | O120
| style="background: #D4E2EF;" | O120
| FliE-F
| FliE-F
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|- style="background: #dddddd;"
| style="background: #D4E2EF;" | O121
| style="background: #D4E2EF;" | O121
| FliE-R
| FliE-R
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|- style="background: #cccccc;"
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|- style="background: #dddddd;"
| style="background: #D4E2EF;" | O122
| style="background: #D4E2EF;" | O122
| FliF-F
| FliF-F
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|- style="background: #cccccc;"
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|- style="background: #dddddd;"
| style="background: #D4E2EF;" | O123
| style="background: #D4E2EF;" | O123
| FliF-R
| FliF-R
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|- style="background: #dddddd;"
| style="background: #D4E2EF;" | O124
| style="background: #D4E2EF;" | O124
| FliL-F
| FliL-F
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|- style="background: #dddddd;"
| style="background: #D4E2EF;" | O125
| style="background: #D4E2EF;" | O125
| FliL-R
| FliL-R
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|- style="background: #cccccc;"
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|- style="background: #dddddd;"
| style="background: #D4E2EF;" | O126
| style="background: #D4E2EF;" | O126
| Gene-EnvZ-F
| Gene-EnvZ-F
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|- style="background: #cccccc;"
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|- style="background: #dddddd;"
| style="background: #D4E2EF;" | O127
| style="background: #D4E2EF;" | O127
| Gene-EnvZ-R
| Gene-EnvZ-R
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|- style="background: #cccccc;"
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|- style="background: #dddddd;"
| style="background: #D4E2EF;" | O128
| style="background: #D4E2EF;" | O128
| Gene-FlgM-F
| Gene-FlgM-F
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|- style="background: #cccccc;"
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|- style="background: #dddddd;"
| style="background: #D4E2EF;" | O129
| style="background: #D4E2EF;" | O129
| Gene-FlgM-R
| Gene-FlgM-R
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|- style="background: #cccccc;"
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|- style="background: #dddddd;"
| style="background: #D4E2EF;" | O130
| style="background: #D4E2EF;" | O130
| Gene-FlhC-F
| Gene-FlhC-F
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|- style="background: #cccccc;"
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|- style="background: #dddddd;"
| style="background: #D4E2EF;" | O131
| style="background: #D4E2EF;" | O131
| Gene-FlhC-R
| Gene-FlhC-R
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|- style="background: #cccccc;"
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|- style="background: #dddddd;"
| style="background: #D4E2EF;" | O132
| style="background: #D4E2EF;" | O132
| Gene-FlhD-F
| Gene-FlhD-F
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|Don't amplify the natural rbs of FlhD
|Don't amplify the natural rbs of FlhD
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|- style="background: #cccccc;"
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|- style="background: #dddddd;"
| style="background: #D4E2EF;" | O133
| style="background: #D4E2EF;" | O133
| Gene-FlhD-R
| Gene-FlhD-R
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|- style="background: #cccccc;"
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|- style="background: #dddddd;"
| style="background: #D4E2EF;" | O134
| style="background: #D4E2EF;" | O134
| Gene-FlhDCrbs-F
| Gene-FlhDCrbs-F
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|Amplify the natural rbs of FlhD so that we have FlhD and FlhC with both their natural rbs
|Amplify the natural rbs of FlhD so that we have FlhD and FlhC with both their natural rbs
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|- style="background: #cccccc;"
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|- style="background: #dddddd;"
| style="background: #D4E2EF;" | O135
| style="background: #D4E2EF;" | O135
| Gene-FliA-F
| Gene-FliA-F
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|Don't Amplify the natural rbs of FliA
|Don't Amplify the natural rbs of FliA
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|- style="background: #cccccc;"
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|- style="background: #dddddd;"
| style="background: #D4E2EF;" | O136
| style="background: #D4E2EF;" | O136
| Gene-FliA-R
| Gene-FliA-R
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|- style="background: #dddddd;"
| style="background: #D4E2EF;" | O137
| style="background: #D4E2EF;" | O137
| Gene-FliArbs-F
| Gene-FliArbs-F
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|Amplify the natural rbs of FliA
|Amplify the natural rbs of FliA
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|- style="background: #cccccc;"
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|- style="background: #dddddd;"
| style="background: #D4E2EF;" | O138
| style="background: #D4E2EF;" | O138
| Gene-OmpR-F
| Gene-OmpR-F
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|- style="background: #dddddd;"
| style="background: #D4E2EF;" | O139
| style="background: #D4E2EF;" | O139
| Gene-OmpR-R
| Gene-OmpR-R

Revision as of 14:39, 30 July 2008

Designed Oligos

Table of Oligos
Number Name Sequence Length Tm(°C) Comments
O100 FlgA-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAGCATATCTCCTCCGCAGGTATCAAAAT 58
O101 FlgA-R GTTTCTTCCTGCAGCGGCCGCTACTAGTAACAGTATCGCGATGATCGCCACGCTACGT 58
O102 FlgB-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGACAGTATCGCGATGATCGCCACGCTACG 58
O103 FlgB-R GTTTCTTCCTGCAGCGGCCGCTACTAGTAAGCATATCTCCTCCGCAGGTATCAAAATT 58
O104 FlgK-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCAGCAGATGAAATCGATAAGCGACAAGG 58
O105 FlgK-R GTTTCTTCCTGCAGCGGCCGCTACTAGTAAACGCCGCCTGGGCCGCGTTCAGTCCGCT 58
O106 FlgM-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAGCGGCGTTGTTGATGCAGATGGGAATA 58
O107 FlgM-R GTTTCTTCCTGCAGCGGCCGCTACTAGTACGAAGTGCGATCAATACTCATGGTTTATT 58
O108 FlhB-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCCACGTCATATCAGGCGGTCTGATAAGG 58
O109 FlhB-R GTTTCTTCCTGCAGCGGCCGCTACTAGTAGTTTTGTCGTCGCTCTCGTCAGACACGTC 58
O110 FlhDC-Uri-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTTGTATGTGCGTGTAGTGACGAGTACAG 58 Don't amplify the both OmpR binding site
O111 FlhDC-Total-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTCATTTTTGCTTGCTAGCGTACGGAAAA 58 Amplify the both OmpR binding site
O112 FlhDC(rbs)-R GTTTCTTCCTGCAGCGGCCGCTACTAGTATCCCACCCAGAATAACCAACTTTAT 54 Amplify the natural rbs of FlhD
O113 FlhDC(nu)-R GTTTCTTCCTGCAGCGGCCGCTACTAGTACAGAATAACCAACTTTATTTTTATG 54 Don't amplify the natural rbs of FlhD (only promoter)
O114 FliA-PCR1-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCCTGATTAACTGAGACTGACGGCAACGC 58
O115 FliA-PCR1-R CAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCCGCAGGGTTTTTAATCG 59
O116 FliA-PCR2-F GAAACGGATAATCATGCCGATAACTCATATAACGCAGGGCTG 42
O117 FliA-PCR2-R GTTTCTTCCTGCAGCGGCCGCTACTAGTACCATTACACCTTCAGCGGTATAGAGTGAACTCACGATAAA 69
O118 FliC-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCCAGCGATGAAATACTTGCCATGCGATT 58
O119 FliC-R GTTTCTTCCTGCAGCGGCCGCTACTAGTACAGCGAGAGGCTGTTGGTATTAATGACTT 58
O120 FliE-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGTCGCATTCATCGCGCACCTCGTGGCTG 58
O121 FliE-R GTTTCTTCCTGCAGCGGCCGCTACTAGTACTGGCTGATAACCCCTTCAATCCCCTGTA 58
O122 FliF-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCTGGCTGATAACCCCTTCAATCCCCTGT 58
O123 FliF-R GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCTGGCTGATAACCCCTTCAATCCCCTGT 58
O124 FliL-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCAGCGAGAGGCTGTTGGTATTAATGACT 58
O125 FliL-R GTTTCTTCCTGCAGCGGCCGCTACTAGTACCAGCGATGAAATACTTGCCATGCGATTT 58
O126 Gene-EnvZ-F GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGGCGATTGCGCTTCTCGCCAC 53
O127 Gene-EnvZ-R GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTACCCTTCTTTTGTCGTGCCCTGCGCC 59
O128 Gene-FlgM-F GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGTATTGATCGCACTTCGCCTC 53
O129 Gene-FlgM-R GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTAGTTACTCTGCAAGTCTTGCTGCGCT 59
O130 Gene-FlhC-F GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGTGAAAAAAGCATTGTTCAGG 53
O131 Gene-FlhC-R GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTAAACAGCCTGTACTCTCTGTTCATCC 59
O132 Gene-FlhD-F GTTTCTTCGAATTCGCGGCCGCTTCTAGATGCATACCTCCGAGTTGCTGAAAC 53 Don't amplify the natural rbs of FlhD
O133 Gene-FlhD-R GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTAGGCCCTTTTCTTGCGCAGCGCTTCT 59
O134 Gene-FlhDCrbs-F GTTTCTTCGAATTCGCGGCCGCTTCTAGACCGCATAAAAATAAAGTTGGTTAT 53 Amplify the natural rbs of FlhD so that we have FlhD and FlhC with both their natural rbs
O135 Gene-FliA-F GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAATTCACTCTATACCGCTGAAG 53 Don't Amplify the natural rbs of FliA
O136 Gene-FliA-R GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTATAACTTACCCAGTTTAGTGCGTAAC 59
O137 Gene-FliArbs-F GTTTCTTCGAATTCGCGGCCGCTTCTAGTAATCATGCCGATAACTCATATAAC 53 Amplify the natural rbs of FliA
O138 Gene-OmpR-F GTTTCTTCGAATTCGCGGCCGCTTCTAGATGCAAGAGAACTACAAGATTCTGG 53
O139 Gene-OmpR-R GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTATGCTTTAGAGCCGTCCGGTACAAAG 59



Retrieved from "http://2008.igem.org/Team:Paris/Notebook/Oligo"