Wisconsin: Lignin Project/10 July 2008

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(New page: == Team Sorbitol ==)
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== Team Sorbitol ==
== Team Sorbitol ==
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Got the sequencing data back and found that colony 2 was correct and will be used for all future experiments.
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Started the following cultures:
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DH5a-PBAD30 : to harvest the plasmid to clone out the ''srl'' operon
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DH5a-pBAD30-srlD: from colony 2 to freeze down for back up
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DH5a-pBAD18 - to insert ''srld'' and the operon into.
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MG1655, JW3890, RL257 - all to make chemically competent

Revision as of 20:28, 31 July 2008

Team Sorbitol

Got the sequencing data back and found that colony 2 was correct and will be used for all future experiments.

Started the following cultures:

DH5a-PBAD30 : to harvest the plasmid to clone out the srl operon DH5a-pBAD30-srlD: from colony 2 to freeze down for back up DH5a-pBAD18 - to insert srld and the operon into.

MG1655, JW3890, RL257 - all to make chemically competent