Wisconsin: Lignin Project/10 July 2008
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== Team Sorbitol == | == Team Sorbitol == | ||
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+ | Got the sequencing data back and found that colony 2 was correct and will be used for all future experiments. | ||
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+ | Started the following cultures: | ||
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+ | DH5a-PBAD30 : to harvest the plasmid to clone out the ''srl'' operon | ||
+ | DH5a-pBAD30-srlD: from colony 2 to freeze down for back up | ||
+ | DH5a-pBAD18 - to insert ''srld'' and the operon into. | ||
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+ | MG1655, JW3890, RL257 - all to make chemically competent |
Revision as of 20:28, 31 July 2008
Team Sorbitol
Got the sequencing data back and found that colony 2 was correct and will be used for all future experiments.
Started the following cultures:
DH5a-PBAD30 : to harvest the plasmid to clone out the srl operon DH5a-pBAD30-srlD: from colony 2 to freeze down for back up DH5a-pBAD18 - to insert srld and the operon into.
MG1655, JW3890, RL257 - all to make chemically competent