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Wisconsin: Lignin Project/10 July 2008
From 2008.igem.org
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DH5a-PBAD30 : to harvest the plasmid to clone out the ''srl'' operon | DH5a-PBAD30 : to harvest the plasmid to clone out the ''srl'' operon | ||
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DH5a-pBAD30-srlD: from colony 2 to freeze down for back up | DH5a-pBAD30-srlD: from colony 2 to freeze down for back up | ||
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DH5a-pBAD18 - to insert ''srld'' and the operon into. | DH5a-pBAD18 - to insert ''srld'' and the operon into. | ||
| + | |||
MG1655, JW3890, RL257 - all to make chemically competent | MG1655, JW3890, RL257 - all to make chemically competent | ||
Revision as of 20:29, 31 July 2008
Team Sorbitol
Got the sequencing data back and found that colony 2 was correct and will be used for all future experiments.
Started the following cultures:
DH5a-PBAD30 : to harvest the plasmid to clone out the srl operon
DH5a-pBAD30-srlD: from colony 2 to freeze down for back up
DH5a-pBAD18 - to insert srld and the operon into.
MG1655, JW3890, RL257 - all to make chemically competent
