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Team:KULeuven/Primers
From 2008.igem.org
(Difference between revisions)
| Line 81: | Line 81: | ||
|Tm=55.3 | |Tm=55.3 | ||
|comments=Reverse primer for sequence analysis | |comments=Reverse primer for sequence analysis | ||
| + | }} | ||
| + | {{:Team:KULeuven/Tools/Oligo | ||
| + | |nr=10xx | ||
| + | |name=antisense luxI F | ||
| + | |sequence= <div class="code">GAATTCGCGG CCGCTTCTAG TTATTAAGCT ACTAAAGCGT AGTTTTC</div> | ||
| + | |length=47bp | ||
| + | |Tm=51.6 | ||
| + | |comments=Forward primer for antisense LuxI | ||
| + | }} | ||
| + | {{:Team:KULeuven/Tools/Oligo | ||
| + | |nr=10xx | ||
| + | |name=antisense luxI R | ||
| + | |sequence= <div class="code">CTGCAGCGGC CGCTACTAGT AATGACTATA ATGATAAAAA AATCGGATTT</div> | ||
| + | |length=50bp | ||
| + | |Tm=50.6 | ||
| + | |comments=Reverse primer for antisense LuxI | ||
}} | }} | ||
|} | |} | ||
Revision as of 07:52, 18 August 2008
dock/undock dropdown
| Table of Oligos | |||||
|---|---|---|---|---|---|
| Number | Name | Sequence | Length | Tm (°C) | Comments |
| 1076 | T7 Fw, no tag | ATGAATTCGC GGCCGCTTCT AGATGAACAC GATTAACATC GC
| 42bp | 66.7 | Forward T7 RNApol primer, without UmuD tag |
| 1077 | T7 Rev | CTGCAGCGGC CGCTACTAGT ATTATTACGC GAACGCGAAG TCCG
| 44bp | 59.9 | Reverse T7 RNApol primer |
| 1078 | T7 Fw, UmuD tag, part 1 | CCGCTATTTA GCGATCTTGT TCAGTGTGGC TTTCCTTCAC CGATGAACAC GATTAACATC GC
| 62bp | 51.0 | Forward T7 RNApol primer, with UmuD tag, part 1 |
| 1079 | T7 Tw, UmuD tag, part 2 | ATGAATTCGC GGCCGCTTCT AGATGTTGTT TATCAAGCCT GCGGATCTCC GCGAAATTGT GACTTTTCCG CTATTTAGCG ATCTTGTTCA G
| 91bp | 55.8 | Forward T7 RNApol primer, with UmuD tag, part 2, very expensive |
| 1084 | GFP-LVA Fw | CATCATGAAT TCGCGGCCGC TTCTAGATGC GTAAAGGAGA AGAACTTTTC ACTGG
| 55bp | 58.9 | Forward GFP-LVA primer |
| 1085 | GFP-LVA Rev | CTGCAGCGGC CGCTACTAGT ATTATTAAGC TACTAAAGCG TAGTTTTCGT CGTTTGCAGC AGGC
| 64bp | 64.9 | Reverse GFP-LVA primer |
| 1074 | VF2 | TGCCACCTGA CGTCTAAGAA
| 20bp | 55.9 | Forward primer for sequence analysis |
| 1075 | VR | ATTACCGCCT TTGAGTGAGC
| 20bp | 55.3 | Reverse primer for sequence analysis |
| 10xx | antisense luxI F | GAATTCGCGG CCGCTTCTAG TTATTAAGCT ACTAAAGCGT AGTTTTC
| 47bp | 51.6 | Forward primer for antisense LuxI |
| 10xx | antisense luxI R | CTGCAGCGGC CGCTACTAGT AATGACTATA ATGATAAAAA AATCGGATTT
| 50bp | 50.6 | Reverse primer for antisense LuxI |
