Team:Paris/Notebook/Freezer

From 2008.igem.org

(Difference between revisions)
(→Plasmids: MiniPrep Products)
(→Plasmids: MiniPrep Products)
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|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" |MP100
| style="background: #D4E2EF;" |MP100
-
|align="center"|[http://partsregistry.org/Part:BBa_B0034 B0034 ] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2]  
+
|align="center"|[http://partsregistry.org/Part:BBa_B0034 B0034 ] in [http://partsregistry.org/Part:pSB1A2 pSB1A2]  
|align="center"|[[Image:Part_icon_rbs.png]]
|align="center"|[[Image:Part_icon_rbs.png]]
|align="center"|Strongest RBS (Efficiency = 1)
|align="center"|Strongest RBS (Efficiency = 1)
Line 77: Line 77:
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" |MP103
| style="background: #D4E2EF;" |MP103
-
|align="center"|[http://partsregistry.org/Part:BBa_R0079 R0079] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2]
+
|align="center"|[http://partsregistry.org/Part:BBa_R0079 R0079] in [http://partsregistry.org/Part:pSB1A2
 +
pSB1A2]
|align="center"|[[Image:Part_icon_regulatory.png]]
|align="center"|[[Image:Part_icon_regulatory.png]]
|align="center"|promoter pLas (lasr & PAI regulated)
|align="center"|promoter pLas (lasr & PAI regulated)
Line 84: Line 85:
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" |MP104
| style="background: #D4E2EF;" |MP104
-
|align="center"|[http://partsregistry.org/Part:BBa_R0040 R0040] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2]
+
|align="center"|[http://partsregistry.org/Part:BBa_R0040 R0040] in [http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[[Image:Part_icon_regulatory.png]]
|align="center"|[[Image:Part_icon_regulatory.png]]
|align="center"|tetR repressible promoter
|align="center"|tetR repressible promoter
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|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" |MP105
| style="background: #D4E2EF;" |MP105
-
|align="center"|[http://partsregistry.org/Part:BBa_S03154 S03154] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2]
+
|align="center"|[http://partsregistry.org/Part:BBa_S03154 S03154] in [http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]
|align="center"|B0034(rbs) ->LasI
|align="center"|B0034(rbs) ->LasI
Line 98: Line 99:
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" |MP106
| style="background: #D4E2EF;" |MP106
-
|align="center"|[http://partsregistry.org/Part:BBa_S03879 S03879] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2]  
+
|align="center"|[http://partsregistry.org/Part:BBa_S03879 S03879] in [http://partsregistry.org/Part:pSB1A2 pSB1A2]  
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]
|align="center"|B0034(rbs) -> TetR  
|align="center"|B0034(rbs) -> TetR  

Revision as of 17:44, 4 August 2008

Contents

PCR products

PCR products
Number Name Oligo F Oligo R Matrix Length Comments Product Left ? []ng/µL Date

Plasmids: MiniPrep Products

Plasmids: MiniPrep Products 2008
Number Name Biobricks Description Products in use Date
MP100 [http://partsregistry.org/Part:BBa_B0034 B0034 ] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.png Strongest RBS (Efficiency = 1) July 26
MP101 [http://partsregistry.org/Part:BBa_J23101 J23101] in [http://partsregistry.org/Part:BBa_J61002 pJ61002 ] Part icon regulatory.png Strongest Constitutive promoter July 22July 23July 26
MP102 [http://partsregistry.org/Part:BBa_J23109 J23109] in [http://partsregistry.org/Part:BBa_J61002 J61002] Part icon regulatory.png Weak Constitutive promoter July 22July 26
MP103 [http://partsregistry.org/Part:BBa_R0079 R0079] in [http://partsregistry.org/Part:pSB1A2

pSB1A2]

Part icon regulatory.png promoter pLas (lasr & PAI regulated) July 23July 26
MP104 [http://partsregistry.org/Part:BBa_R0040 R0040] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon regulatory.png tetR repressible promoter July 23July 26
MP105 [http://partsregistry.org/Part:BBa_S03154 S03154] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngIcon coding.png B0034(rbs) ->LasI July 26
MP106 [http://partsregistry.org/Part:BBa_S03879 S03879] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngIcon coding.png B0034(rbs) -> TetR July 26
MP107 [http://partsregistry.org/Part:BBa_C0079 C0079] in [http://partsregistry.org/Part:BBa_SB2K3 pSB2K3] Icon coding.png lasR activator with LVA Tag July 23
MP108 [http://partsregistry.org/Part:BBa_C0179 C0179] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] Icon coding.png lasR activator July 22July 23July 26
MP109 [http://partsregistry.org/Part:BBa_E0030 E0030] in [http://partsregistry.org/Part:BBa_SB1AK3 pSB1AK3] Part icon reporter.png YFP July 26
MP110 [http://partsregistry.org/Part:BBa_E0040 E0040] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] Part icon reporter.png GFP July 23July 26
MP111 [http://partsregistry.org/Part:BBa_E1010 E1010 pSB2K3] Part icon reporter.png mRFP July 23July 26
MP112 OmpR M571 Icon coding.png July 23
MP113 EnvZ I2815 Icon coding.png July 23
MP114 [http://partsregistry.org/Part:BBa_C0040 C0040] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] Icon coding.png tetR repressor July 23
MP115 [http://partsregistry.org/Part:pSB1A3 pSB1A3] Icon plasmid.png July 23
MP116 [http://partsregistry.org/Part:BBa_J23100 pJ23100] in [http://partsregistry.org/Part:BBa_J61002 J61002] Part icon regulatory.png Strong constitutive promoter in J61002 July 26
MP117 [http://partsregistry.org/Part:BBa_J23107 pJ23107] in [http://partsregistry.org/Part:BBa_J61002 J61002] Part icon regulatory.png Medium constitutive promoter in J61002 July 24July 26
MP118 [http://partsregistry.org/Part:BBa_B0015 B0015] in [http://partsregistry.org/Part:BBa_SB1AK3 pSB1AK3] Part icon terminator.pngPart icon terminator.png Double terminator July 24July 26
MP119 [http://partsregistry.org/Part:BBa_I0500 I0500] in [http://partsregistry.org/Part:BBa_SB2K3 pSB2K3] Part icon regulatory.png AraC pBAD July 24July 26
MP120 [http://partsregistry.org/Part:BBa_B0030 B0030] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] Part icon rbs.png Strong RBS (Efficiency = 0,6) July 26
MP121 [http://partsregistry.org/Part:BBa_E0422 E0422] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] Part icon rbs.pngPart icon reporter.pngPart icon terminator.png ECFP (RBS+LVA+Term) July 24July 26
MP122 [http://partsregistry.org/Part:BBa_E0840 E0840] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] Part icon rbs.pngPart icon reporter.pngPart icon terminator.png gfp tri-part; strong rbs July 26

Digestion Products

(Legend: Vector, Insert)

Digestion Products
Number Product Name Matrix Name Enzyme 1 Enzyme 2 Obs Size Matrix Size BB Description Product Left []ng/µL Date
D100.1 [http://partsregistry.org/Part:BBa_B0034 B0034] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 34 10 July 26
D100.2 [http://partsregistry.org/Part:BBa_B0034 B0034] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 34 10 July 26
D101 [http://partsregistry.org/Part:BBa_B0034 B0034] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI XbaI FV 2076 15 10 July 29
D102.1 [http://partsregistry.org/Part:BBa_B0034 B0034] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2077 14 10 July 26
D102.2 [http://partsregistry.org/Part:BBa_B0034 B0034] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2077 14 10 July 29
D103.1 [http://partsregistry.org/Part:BBa_J23101 J23101] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2100 883 10 July 26
D103.2 [http://partsregistry.org/Part:BBa_J23101 J23101] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2100 883 10 July 26
D104.1 [http://partsregistry.org/Part:BBa_J23109 J23109] [http://partsregistry.org/Part:BBa_J61002 J61002] SpeI PstI BV 2100 883 20 July 26
D104.2 [http://partsregistry.org/Part:BBa_J23109 J23109] [http://partsregistry.org/Part:BBa_J61002 J61002] SpeI PstI BV 2100 883 20 July 26
D105.1 [http://partsregistry.org/Part:BBa_R0079 R0079] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2222 14 5 July 26
D105.2 [http://partsregistry.org/Part:BBa_R0079 R0079] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2222 14 5 July 29
D106.1 [http://partsregistry.org/Part:BBa_R0040 R0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2119 14 8 July 26
D106.2 [http://partsregistry.org/Part:BBa_R0040 R0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2119 14 5 July 29
D107 [http://partsregistry.org/Part:BBa_S03154 S03154] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2750 14 5 July 26
D108.1 [http://partsregistry.org/Part:BBa_S03154 S03154] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 707 5 July 26
D108.2 [http://partsregistry.org/Part:BBa_S03154 S03154] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 707 5 July 29
D109 [http://partsregistry.org/Part:BBa_S03154 S03154] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 2056 708 5 July 26
D110 [http://partsregistry.org/Part:BBa_S03879 S03879] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2768 14 20 July 26
D111.1 [http://partsregistry.org/Part:BBa_S03879 S03879] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 725 20 July 26
D111.2 [http://partsregistry.org/Part:BBa_S03879 S03879] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 725 20 July 29
D112 [http://partsregistry.org/Part:BBa_S03879 S03879] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 2756 726 20 July 26
D113 [http://partsregistry.org/Part:BBa_C0079 C0079] [http://partsregistry.org/Part:pSB2K3 pSB2K3] EcoRI SpeI FI 4402 779 20 July 26
D114 [http://partsregistry.org/Part:BBa_C0079 C0079] [http://partsregistry.org/Part:pSB2K3 pSB2K3] XbaI PstI BI 4003 778 20 July 26
D115.1 [http://partsregistry.org/Part:BBa_C0179 C0179] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 4402 746 - July 29
D115.2 [http://partsregistry.org/Part:BBa_C0179 C0179] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 4402 746 - July 29
D116.1 [http://partsregistry.org/Part:BBa_C0079 C0079] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 4403 745 20 July 29
D116.1 [http://partsregistry.org/Part:BBa_C0079 C0079] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 4403 745 20 July 29
D117 [http://partsregistry.org/Part:BBa_E0030 E0030] [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] EcoRI SpeI FI 3166 746 5 July 29
D118 [http://partsregistry.org/Part:BBa_E0030 E0030] [http://partsregistry.org/Part:pSB2K3 pSB2K3] XbaI PstI BI 3167 745 20 July 26
D119 [http://partsregistry.org/Part:BBa_E0040 E0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 2056 743 10 July 26
D120 [http://partsregistry.org/Part:BBa_E0040 E0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 742 10 July 26
D121 [http://partsregistry.org/Part:BBa_E1010 E1010] [http://partsregistry.org/Part:pSB2K3 pSB2K3] EcoRI SpeI FI 4402 704 10 July 26
D122 [http://partsregistry.org/Part:BBa_E0040 E0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 4403 703 10 July 26
D123.1 [http://partsregistry.org/Part:BBa_J23100 J23100] [http://partsregistry.org/Part:J61002 J61002] SpeI PstI BV 2100 883 10 July 26
D123.2 [http://partsregistry.org/Part:BBa_J23100 J23100] [http://partsregistry.org/Part:J61002 J61002] SpeI PstI BV 2100 883 10 July 26
D124.1 [http://partsregistry.org/Part:BBa_J23107 J23107] [http://partsregistry.org/Part:J61002 J61002] SpeI PstI BV 2100 883 15 July 26
D124.2 [http://partsregistry.org/Part:BBa_J23107 J23107] [http://partsregistry.org/Part:J61002 J61002] SpeI PstI BV 2100 883 15 July 26
D125.1 [http://partsregistry.org/Part:BBa_B0015 B0015] [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] EcoRI XbaI BV 3303 15 15 July 26
D125.2 [http://partsregistry.org/Part:BBa_B0015 B0015] [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] EcoRI XbaI BV 3303 15 15 July 26
D126.1 [http://partsregistry.org/Part:BBa_I0500 I0500] [http://partsregistry.org/Part:pSB2K3 pSB2K3] SpeI PstI BV 5621 14 10 July 26
D126.2 [http://partsregistry.org/Part:BBa_I0500 I0500] [http://partsregistry.org/Part:pSB2K3 pSB2K3] SpeI PstI BV 5621 14 10 July 26
D127.1 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 37 10 July 26
D127.2 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 37 10 July 26
D128.1 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI XbaI FV 2079 15 10 July 29
D128.1 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI XbaI FV 2079 15 10 July 29
D129.1 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2080 14 10 July 29
D129.2 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2080 14 10 July 29
D130.1 [http://partsregistry.org/Part:BBa_E0422 E0422] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 939 10 July 26
D130.2 [http://partsregistry.org/Part:BBa_E0422 E0422] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 939 10 July 26
D130.3 [http://partsregistry.org/Part:BBa_E0422 E0422] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 939 10 July 26
D131.1 [http://partsregistry.org/Part:BBa_E0840 E0840] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 900 10 July 26
D131.2 [http://partsregistry.org/Part:BBa_E0840 E0840] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 900 10 July 26
D131.3 [http://partsregistry.org/Part:BBa_E0840 E0840] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 900 10 July 26

Strains (glycerol stock)

Strains glycerol stock
Number Name Description Verified clone Date
S103 [http://partsregistry.org/Part:BBa_I0500 I0500] AraC pBAD
Part icon regulatory.png 		July 23
S106 [http://partsregistry.org/Part:BBa_E0422 E0422] ECFP (RBS+LVA+Term)
Part icon rbs.pngPart icon reporter.pngPart icon terminator.png 		July 23
S107 [http://partsregistry.org/Part:BBa_B0034 B0034] Strongest RBS (Efficiency = 1)
Part icon rbs.png 		July 23
S108 [http://partsregistry.org/Part:BBa_R0079 R0079] promoter pLas (lasr & PAI regulated)
Part icon regulatory.png 		July 23
S109 [http://partsregistry.org/Part:BBa_J23101 J23101] Strongest Constitutive promoter
Part icon regulatory.png 		July 23
S110 [http://partsregistry.org/Part:BBa_J23109 J23109] Weak Constitutive promoter
Part icon regulatory.png 		July 23
S111 [http://partsregistry.org/Part:BBa_R0040 R0040] tetR repressible promoter
Part icon regulatory.png 		July 23
S112 [http://partsregistry.org/Part:BBa_S03154 S03154] B0034(rbs) ->LasI
Part icon rbs.pngIcon coding.png 		July 23
S113 [http://partsregistry.org/Part:BBa_S03879 S03879] B0034(rbs) -> TetR
Part icon rbs.pngIcon coding.png 		July 23
S114 [http://partsregistry.org/Part:BBa_C0079 C0079] lasR activator with LVA Tag
Icon coding.png 		July 23
S115 [http://partsregistry.org/Part:BBa_C0179 C0179] lasR activator
Icon coding.png 		July 23
S116 [http://partsregistry.org/Part:BBa_E0030 E0030] YFP
Part icon reporter.png 		July 23
S117 [http://partsregistry.org/Part:BBa_E0040 E0040] GFP
Part icon reporter.png 		July 23
S118 [http://partsregistry.org/Part:BBa_E1010 E1010] mRFP
Part icon reporter.png 		July 23
S119 OmpR M571 Icon coding.png July 23
S120 EnvZ I2815 Icon coding.png July 23
S121 [http://partsregistry.org/Part:BBa_C0040 C0040] tetR repressor
Icon coding.png 		July 23
S122 [http://partsregistry.org/Part:pSB1A3 pSB1A3] Icon plasmid.png July 23
S123 L104 Part icon rbs.pngIcon coding.png

Strong rbs - lasR activator
D129 (BV) - D114 (BI)

Aug 1
S124 L105 Part icon regulatory.pngPart icon reporter.png

Strong promoter - ECFP
D123 (BV) - D130 (BI)

Aug 1
S125 L106 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.png

Strong promoter - gfp Tripart
D123 (BV) - D131 (BI)

Aug 1
S126 L107 Part icon regulatory.pngPart icon reporter.png

Strongest promoter - ECFP
D103 (BV) - D131 (BI)

Aug 1
S127 L108 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.png

Strong promoter - gfp Tripart
D103 (BV) - D130 (BI)

Aug 1
S128 L110 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.png

Medium promoter - gfp Tripart
D124 (BV) - D131 (BI)

Aug 1
S129 L111 Part icon regulatory.pngPart icon reporter.png

Weak promoter - ECFP
D104 (BV) - D130 (BI)

Aug 1
S130 L112 Part icon regulatory.pngPart icon reporter.png

Weak promoter - gfp
D104 (BV) - D131 (BI)

Aug 1
S131 L115 Part icon regulatory.pngPart icon reporter.png

pLas - ECFP
D105 (BV) - D130 (BI)

Aug 1
S132 L116 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.png

pLas - gfp Tripart
D105 (BV) - D131 (BI)

Aug 1
S133 L117 Part icon terminator.pngPart icon terminator.pngPart icon reporter.png

Double Terminator - yfp
D125 (FV) - D117 (FI)

Aug 1
S134 L118 Part icon terminator.pngPart icon terminator.pngPart icon reporter.png

Double Terminator - rfp
D125 (FV) - D121 (FI)

Aug 1
S135 L119 Part icon terminator.pngPart icon terminator.pngIcon coding.png

Double Terminator - lasR activator + LVA
D125 (FV) - D113 (FI)

Aug 1
S136 L121 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.png

Strong promoter - gfp tripart
D106 (BV) - D130 (BI)

Aug 1
S137 L124 Part icon rbs.pngPart icon reporter.png

Strongest RBS - rfp
D102 (BV) - D122 (BI)

Aug 1
S138 L125 Part icon rbs.pngPart icon reporter.png

Strongest RBS - yfp
D102 (BV) - D118 (BI)

Aug 1
Retrieved from "http://2008.igem.org/Team:Paris/Notebook/Freezer"