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Essential publications

Project essentials

• Caroline M. Ajo-Franklin et al. Rational design of memory in eukaryotic cells. (2007) PMID 17875664
• Gudrun Bjornsdottir and Lawrence C. Myers. Minimal components of the RNA polymerase II transcription apparatus determine the consensus TATA box. (2008) PMID 18385157
• Christina B. Tyson et al. Dependency of size of Saccharomyces cerevisiae cells on growth rate. (1978) PMID 374379

Eukaryotic activation/repression domains

• Gemma Bellí et al. An activator/repressor dual system allows tight tetracycline-regulated gene expression in budding yeast. (1998) PMID 9461451
• Jae-Ryeon Ryu et al. Cell-type specificity of short-range transcriptional repressors. (2001) PMID 11687630
• Tania M. Malavé and Sharon Y.R. Dent. Transcriptional repression by Tup1-Ssn6. (2006) PMID 16936817

Prokaryotic DNA binding domains (+ affinity mutations)

• Christian Berens and Wolfgang Hillen. Gene regulation by tetracyclines. Constraints of resistance regulation in bacteria shape TetR for application in eukaryotes. (2003) PMID 12869186
• A. Wissmann et al. Amino acids determining operator binding specificity in the helix-turn-helix motif of Tn10 Tet repressor. (1991) PMID 1756721

Protein degradation motifs

• Emily R. Hildebrandt and M. Andrew Hoyt. Cell cycle-dependent degradation of the Saccharomyces cerevisiae spindle motor Cin8p requires APC(Cdh1) and a bipartite destruction sequence. (2001) PMID 11694576
• Janet L. Burton and Mark Solomon. D box and KEN box motifs in budding yeast Hsl1p are required for APC-mediated degradation and direct binding to Cdc20p and Cdh1p. (2001) PMID 11562348
• Sofie R. Salama. G1 cyclin degradation: the PEST motif of yeast Cln2 is necessary, but not sufficient, for rapid protein turnover. (1994) PMID 7969135
• Carolina Mateus and Simon V. Avery. Destabilized green fluorescent protein for monitoring dynamic changes in yeast gene expression with flow cytometry. (2000) PMID 11015728

Nuclear localistation

• Julia M. Sidorova et al. Cell cycle-regulated phosphorylation of Swi6 controls its nuclear localization. (1995) PMID 8590795
• Michelle T. Harreman. Regulation of nuclear import by phosphorylation adjacent to nuclear localization signals. (2004) PMID 14998990

Site-specific proteases

• Sung Yun Kim et al. In vivo determination of substrate specificity of hepatitis C virus NS3 protease: genetic assay for site-specific proteolysis. (2000) PMID 10933854
• Hara Kang et al. An improved strategy for a genetic assay for site-specific proteolysis. (2001) PMID 11355710

mRNA instability

• Reut Shalgi et al. A catalog of stability-associated sequence elements in 3'UTRs of yeast mRNAs. (2005) PMID 16207357

BioBricks assembly

• Tom Knight. Idempotent Vector Design for Standard Assembly of Biobricks (2003) [http://web.mit.edu/synbio/release/docs/biobricks.pdf]
• Ira E. Philips and Pamels A. Silver. A New Biobrick Assembly Strategy Designed for Facile Protein Engineering (2006) [http://dspace.mit.edu/bitstream/handle/1721.1/32535/PhillipsSilverFusion.pdf?sequence=1]

Toggle switches

• Timothy S. Gardner et al. Construction of a genetic toggle switch in Escherichia coli. (2000) PMID 10659857

Fluorescent proteins

• Nathan C. Shaner et al. A guide to choosing fluorescent proteins. (2005) PMID 16299475