Team:Paris/Notebook/Freezer

From 2008.igem.org

Revision as of 17:56, 13 August 2008

Contents 1 PCR products 2 Plasmids: MiniPrep Products 3 Digestion Products 4 Strains (glycerol stock) PCR products PCR products Number Name Oligo F Oligo R Matrix Length Comments Product Left ? []ng/µL Date PCR123 pFlgA O100 O101 - 261pb yes 500 August 7 PCR124 pFlgB O102 O103 - 261pb yes 500 August 7 PCR125 pFlhB O108 O109 - 260pb yes 500 August 7 Plasmids: MiniPrep Products Plasmids: MiniPrep Products 2008 Number Name Biobricks Description Products in use Date MP100 [http://partsregistry.org/Part:BBa_B0034 B0034 ] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Strongest RBS (Efficiency = 1) July 26 MP101 [http://partsregistry.org/Part:BBa_J23101 J23101] in [http://partsregistry.org/Part:BBa_J61002 pJ61002 ] Strongest Constitutive promoter July 26 July 22, July 23, July 26 MP102 [http://partsregistry.org/Part:BBa_J23109 J23109] in [http://partsregistry.org/Part:BBa_J61002 pJ61002 ] Weak Constitutive promoter July 26 July 22, July 26 MP103 [http://partsregistry.org/Part:BBa_R0079 R0079] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] promoter pLas (lasr & PAI regulated) July 26 July 23, July 26 MP104 [http://partsregistry.org/Part:BBa_R0040 R0040] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] tetR repressible promoter July 26 July 23, July 26 MP105 [http://partsregistry.org/Part:BBa_S03154 S03154] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] B0034(rbs) ->LasI July 26 MP106 [http://partsregistry.org/Part:BBa_S03879 S03879] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] B0034(rbs) -> TetR July 26 MP107 [http://partsregistry.org/Part:BBa_C0079 C0079] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] lasR activator with LVA Tag July 23 MP108 [http://partsregistry.org/Part:BBa_C0179 C0179] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] lasR activator July 26 July 22, July 23, July 26 MP109 [http://partsregistry.org/Part:BBa_E0030 E0030] in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] YFP July 26 MP110 [http://partsregistry.org/Part:BBa_E0040 E0040] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] GFP July 26 July 23, July 26 MP111 [http://partsregistry.org/Part:BBa_E1010 E1010 ] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] mRFP July 26 July 23, July 26 MP112 OmpR M571 July 23 MP113 EnvZ I2815 July 23 MP114 [http://partsregistry.org/Part:BBa_C0040 C0040] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] tetR repressor July 23 MP115 [http://partsregistry.org/Part:pSB1A3 pSB1A3] July 23 MP116 [http://partsregistry.org/Part:BBa_J23100 pJ23100] in [http://partsregistry.org/Part:BBa_J61002 J61002] Strong constitutive promoter in J61002 July 26 MP117 [http://partsregistry.org/Part:BBa_J23107 pJ23107] in [http://partsregistry.org/Part:BBa_J61002 J61002] Medium constitutive promoter in J61002 July 26 July 24, July 26 MP118 [http://partsregistry.org/Part:BBa_B0015 B0015] in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] Double terminator July 26 July 24, July 26 MP119 [http://partsregistry.org/Part:BBa_I0500 I0500] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] AraC pBAD July 26 July 24, July 26 MP120 [http://partsregistry.org/Part:BBa_B0030 B0030] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Strong RBS (Efficiency = 0,6) July 26 MP121 [http://partsregistry.org/Part:BBa_E0422 E0422] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] ECFP (RBS+LVA+Term) July 26 July 24, July 26 MP122 [http://partsregistry.org/Part:BBa_E0840 E0840] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] gfp tri-part; strong rbs July 26 MP123 [http://partsregistry.org/Part:J61002 J61002] August 5 MP124 L102 Strong rbs - YFP D129 (BV) - D118 (BI) August 1 MP125 L103 Strong rbs - mRFP D129 (BV) - D122 (BI) August 1 MP126 L104 Strong rbs - lasR activator D129 (BV) - D114 (BI) August 1 MP127 L105 Strong promoter - ECFP D123 (BV) - D130 (BI) August 1 MP128 L106 Strong promoter - gfp Tripart D123 (BV) - D131 (BI) August 1 MP129 L107 Strong promoter - ECFP D103 (BV) - D130 (BI) August 1 MP130 L108 Strong promoter - gfp Tripart D103 (BV) - D131 (BI) August 1 MP131 L110 Medium promoter - gfp Tripart D124 (BV) - D131 (BI) August 1 MP132 L111 Weak promoter - ECFP D104 (BV) - D130 (BI) August 1 MP133 L112 Weak promoter - GFP tripart D104 (BV) - D131 (BI) August 1 MP134 L115 pLas - ECFP D105 (BV) - D130 (BI) August 1 MP135 L116 pLas - gfp Tripart D105 (BV) - D131 (BI) August 1 MP136 L117 yfp - Double Terminator D125 (FV) - D117 (FI) August 1 MP137 L118 rfp - Double Terminator D125 (FV) - D121 (FI) August 1 MP138 L119 lasR activator + LVA - Double Terminator D125 (FV) - D113 (FI) August 1 MP139 L121 pTet - gfp tripart D106 (BV) - D131 (BI) August 1 MP140 L124 Strongest RBS - rfp D102 (BV) - D122 (BI) August 1 MP141 L125 Strongest RBS - yfp D102 (BV) - D118 (BI) August 1 MP144 L126 pFlgA-RFP D132 (FI) - D136 (FV) August 12 MP145 L127 pFlgB-RFP D133 (FI) - D136 (FV) August 12 Digestion Products (Legend: Vector, Insert) Ø Digestion Products Number Product Name Matrix Name Enzyme 1 Enzyme 2 Obs Size Matrix Size BB Description Product Left []ng/µL Date D100.1 [http://partsregistry.org/Part:BBa_B0034 B0034] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 34 10 July 26 D100.2 [http://partsregistry.org/Part:BBa_B0034 B0034] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 34 10 July 26 D101 [http://partsregistry.org/Part:BBa_B0034 B0034] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI XbaI FV 2076 15 10 July 29 D102.1 [http://partsregistry.org/Part:BBa_B0034 B0034] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2077 14 10 July 26 D102.2 [http://partsregistry.org/Part:BBa_B0034 B0034] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2077 14 10 July 29 D103.1 [http://partsregistry.org/Part:BBa_J23101 J23101] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2100 883 10 July 26 D103.2 [http://partsregistry.org/Part:BBa_J23101 J23101] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2100 883 10 July 26 D104.1 [http://partsregistry.org/Part:BBa_J23109 J23109] [http://partsregistry.org/Part:BBa_J61002 J61002] SpeI PstI BV 2100 883 20 July 26 D104.2 [http://partsregistry.org/Part:BBa_J23109 J23109] [http://partsregistry.org/Part:BBa_J61002 J61002] SpeI PstI BV 2100 883 20 July 26 D105.1 [http://partsregistry.org/Part:BBa_R0079 R0079] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2222 14 5 July 26 D105.2 [http://partsregistry.org/Part:BBa_R0079 R0079] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2222 14 5 July 29 D106.1 [http://partsregistry.org/Part:BBa_R0040 R0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2119 14 8 July 26 D106.2 [http://partsregistry.org/Part:BBa_R0040 R0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2119 14 5 July 29 D107 [http://partsregistry.org/Part:BBa_S03154 S03154] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2750 14 5 July 26 D108.1 [http://partsregistry.org/Part:BBa_S03154 S03154] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 707 5 July 26 D108.2 [http://partsregistry.org/Part:BBa_S03154 S03154] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 707 5 July 29 D109 [http://partsregistry.org/Part:BBa_S03154 S03154] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 2056 708 5 July 26 D110 [http://partsregistry.org/Part:BBa_S03879 S03879] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2768 14 20 July 26 D111.1 [http://partsregistry.org/Part:BBa_S03879 S03879] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 725 20 July 26 D111.2 [http://partsregistry.org/Part:BBa_S03879 S03879] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 725 20 July 29 D112 [http://partsregistry.org/Part:BBa_S03879 S03879] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 2756 726 20 July 26 D113 [http://partsregistry.org/Part:BBa_C0079 C0079] [http://partsregistry.org/Part:pSB2K3 pSB2K3] EcoRI SpeI FI 4402 779 20 July 26 D114 [http://partsregistry.org/Part:BBa_C0079 C0079] [http://partsregistry.org/Part:pSB2K3 pSB2K3] XbaI PstI BI 4003 778 20 July 26 D115.1 [http://partsregistry.org/Part:BBa_C0179 C0179] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 4402 746 - July 29 D115.2 [http://partsregistry.org/Part:BBa_C0179 C0179] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 4402 746 - July 29 D116.1 [http://partsregistry.org/Part:BBa_C0079 C0079] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 4403 745 20 July 29 D116.1 [http://partsregistry.org/Part:BBa_C0079 C0079] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 4403 745 20 July 29 D117 [http://partsregistry.org/Part:BBa_E0030 E0030] [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] EcoRI SpeI FI 3166 746 5 July 29 D118 [http://partsregistry.org/Part:BBa_E0030 E0030] [http://partsregistry.org/Part:pSB2K3 pSB2K3] XbaI PstI BI 3167 745 20 July 26 D119 [http://partsregistry.org/Part:BBa_E0040 E0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 2056 743 10 July 26 D120 [http://partsregistry.org/Part:BBa_E0040 E0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 742 10 July 26 D121 [http://partsregistry.org/Part:BBa_E1010 E1010] [http://partsregistry.org/Part:pSB2K3 pSB2K3] EcoRI SpeI FI 4402 704 10 July 26 D122 [http://partsregistry.org/Part:BBa_E0040 E0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 4403 703 10 July 26 D123.1 [http://partsregistry.org/Part:BBa_J23100 J23100] [http://partsregistry.org/Part:J61002 J61002] SpeI PstI BV 2100 883 10 July 26 D123.2 [http://partsregistry.org/Part:BBa_J23100 J23100] [http://partsregistry.org/Part:J61002 J61002] SpeI PstI BV 2100 883 10 July 26 D124.1 [http://partsregistry.org/Part:BBa_J23107 J23107] [http://partsregistry.org/Part:J61002 J61002] SpeI PstI BV 2100 883 15 July 26 D124.2 [http://partsregistry.org/Part:BBa_J23107 J23107] [http://partsregistry.org/Part:J61002 J61002] SpeI PstI BV 2100 883 15 July 26 D125.1 [http://partsregistry.org/Part:BBa_B0015 B0015] [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] EcoRI XbaI BV 3303 15 15 July 26 D125.2 [http://partsregistry.org/Part:BBa_B0015 B0015] [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] EcoRI XbaI BV 3303 15 15 July 26 D126.1 [http://partsregistry.org/Part:BBa_I0500 I0500] [http://partsregistry.org/Part:pSB2K3 pSB2K3] SpeI PstI BV 5621 14 10 July 26 D126.2 [http://partsregistry.org/Part:BBa_I0500 I0500] [http://partsregistry.org/Part:pSB2K3 pSB2K3] SpeI PstI BV 5621 14 10 July 26 D127.1 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 37 10 July 26 D127.2 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 37 10 July 26 D128.1 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI XbaI FV 2079 15 10 July 29 D128.1 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI XbaI FV 2079 15 10 July 29 D129.1 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2080 14 10 July 29 D129.2 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2080 14 10 July 29 D130.1 [http://partsregistry.org/Part:BBa_E0422 E0422] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 939 10 July 26 D130.2 [http://partsregistry.org/Part:BBa_E0422 E0422] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 939 10 July 26 D130.3 [http://partsregistry.org/Part:BBa_E0422 E0422] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 939 10 July 26 D131.1 [http://partsregistry.org/Part:BBa_E0840 E0840] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 900 10 July 26 D131.2 [http://partsregistry.org/Part:BBa_E0840 E0840] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 900 10 July 26 D131.3 [http://partsregistry.org/Part:BBa_E0840 E0840] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 900 10 July 26 D132 pFlgA - EcoRI SpeI FI - 5 August 6 D133 pFlgB - EcoRI SpeI FI - 5 August 6 D134 pFlhB - EcoRI SpeI FI - 5 August 6 D135 pFlhDC - EcoRI SpeI FI - 5 August 6 D136 - [http://partsregistry.org/Part:BBa_J61002 J61002] EcoRI SpeI FV - 3 August 6 Strains (glycerol stock) Strains glycerol stock Number Name Biobricks Description Verified clone Date S103 [http://partsregistry.org/Part:BBa_I0500 I0500] in [http://partsregistry.org/Part:BBa_SB2K3 pSB2K3] AraC pBAD July 23 S106 [http://partsregistry.org/Part:BBa_E0422 E0422] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] ECFP (RBS+LVA+Term July 23 S107 [http://partsregistry.org/Part:BBa_B0034 B0034] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Strongest RBS (Efficiency = 1) July 23 S108 [http://partsregistry.org/Part:BBa_R0079 R0079] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] promoter pLas (lasr & PAI regulated) July 23 S109 [http://partsregistry.org/Part:BBa_J23101 J23101] in [http://partsregistry.org/Part:BBa_J61002 pJ61002 ] Strongest Constitutive promoter July 23 S110 [http://partsregistry.org/Part:BBa_J23109 J23109] in [http://partsregistry.org/Part:BBa_J61002 pJ61002 ] [Image:Part_icon_regulatory.png]] Weak Constitutive promoter July 23 S111 [http://partsregistry.org/Part:BBa_R0040 R0040] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] tetR repressible promoter July 23 S112 [http://partsregistry.org/Part:BBa_S03154 S03154] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] B0034(rbs) ->LasI July 23 S113 [http://partsregistry.org/Part:BBa_S03879 S03879] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] B0034(rbs) -> TetR July 23 S114 [http://partsregistry.org/Part:BBa_C0079 C0079] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] lasR activator with LVA Tag July 23 S115 [http://partsregistry.org/Part:BBa_C0179 C0179] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] lasR activator July 23 S116 [http://partsregistry.org/Part:BBa_E0030 E0030] in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] YFP July 23 S117 [http://partsregistry.org/Part:BBa_E0040 E0040] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] GFP July 23 S118 [http://partsregistry.org/Part:BBa_E1010 E1010] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] mRFP July 23 S119 OmpR M571 July 23 S120 EnvZ I2815 July 23 S121 [http://partsregistry.org/Part:BBa_C0040 C0040] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] tetR repressor July 23 S122 [http://partsregistry.org/Part:pSB1A3 pSB1A3] July 23 S123 L102 Strong rbs - YFP D129 (BV) - D118 (BI) Aug 1 S124 L103 Image:Part_icon_rbs.png]] Strong rbs - GFP D129 (BV) - D122 (BI) Aug 1 S125 L104 Strong rbs - lasR activator D129 (BV) - D114 (BI) Aug 1 S126 L105 Strong promoter - ECFP D123 (BV) - D130 (BI) Aug 1 S127 L106 Strong promoter - gfp Tripart D123 (BV) - D131 (BI) Aug 1 S128 L107 Strongest promoter - gfp Tripart D103 (BV) - D131 (BI) Aug 1 S129 L108 |align="center"|Weak promoter - ECFP D103 (BV) - D130 (BI) Aug 1 S130 L110 Medium promoter - gfp D124 (BV) - D131 (BI) Aug 1 S131 L111 Weak promoter - ECFP D104 (BV) - D130 (BI) Aug 1 S132 L112 Weak promoter - gfp Tripart D104 (BV) - D131 (BI) Aug 1 S133 L115 pLas - ECFP D105 (BV) - D130 (BI) Aug 1 S134 L116 pLas - gfp Tripart D105 (BV) - D131 (BI) Aug 1 S135 L117 yfp - Double Terminator D125 (FV) - D117 (FI) $|align="center"| Aug 1 S136 L118 rfp - Double Terminator D125 (FV) - D117 (FI) Aug 1 S137 L119 LasR activator + LVA - Double terminator D125 (FV) - D113 (FI) Aug 1 S138 L121 Strong promoter - gfp Tripart D106 (BV) - D130 (BI) Aug 1 S139 L124 Strongest rbs - gfp D102 (BV) - D122 (BI) Aug 1 S140 L125 Strongest rbs - yfp D102 (BV) - D118 (BI) Aug 1

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