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Edinburgh/11 July 2008
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| - | * Submitted M19, M21 and M22 for sequencing using primer pTG262f1, to check for clean removal of the EcoRI site. | + | </style> |
| - | * Revived ''Cellulomonas fimi'' in NB and plated to NA and NA+glucose. '''Plates 28 and 29''' incubated at 30°C. | + | </html> |
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| + | :::: '''[[Edinburgh/10_July_2008|< Previous Entry]]''' | ||
| + | |||
| + | == Week 4 == | ||
| + | === Friday 11 July 08 === | ||
| + | |||
| + | * Submitted M19, M21 and M22 (BABEL2+''glgC''-mut2) for sequencing using primer pTG262f1, to check for clean removal of the EcoRI site. | ||
| + | * Revived ''Cellulomonas fimi'' in Nutrient Broth (NB) and plated to Nutrient Agar (NA) and NA+glucose. '''Plates 28 and 29''' incubated at 30°C. | ||
| + | * Repeated rbs+''dxs'' ligation ('''L11'''). (Yan, HX) | ||
* Transformation of JM109 with L9 (pSB1A2+''crtB'' )and L10 (pSB1A2+''crtI''-mut). '''Plates 30 to 33'''. | * Transformation of JM109 with L9 (pSB1A2+''crtB'' )and L10 (pSB1A2+''crtI''-mut). '''Plates 30 to 33'''. | ||
| - | * possible rbs+''dxs'' clones from | + | * possible rbs+''dxs'' clones from Nimisha's plates were streaked to '''plate 34'''.<br /> |
| + | <br /> | ||
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| + | :::: '''[[Edinburgh/12_July_2008|Next Entry >]]''' | ||
Revision as of 12:04, 21 August 2008
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Week 4
Friday 11 July 08
- Submitted M19, M21 and M22 (BABEL2+glgC-mut2) for sequencing using primer pTG262f1, to check for clean removal of the EcoRI site.
- Revived Cellulomonas fimi in Nutrient Broth (NB) and plated to Nutrient Agar (NA) and NA+glucose. Plates 28 and 29 incubated at 30°C.
- Repeated rbs+dxs ligation (L11). (Yan, HX)
- Transformation of JM109 with L9 (pSB1A2+crtB )and L10 (pSB1A2+crtI-mut). Plates 30 to 33.
- possible rbs+dxs clones from Nimisha's plates were streaked to plate 34.
