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Edinburgh/11 July 2008
From 2008.igem.org
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Week 4
Friday 11 July 08
- Submitted M19, M21 and M22 (BABEL2+glgC-mut2) for sequencing using primer pTG262f1, to check for clean removal of the EcoRI site.
- Revived Cellulomonas fimi in Nutrient Broth (NB) and plated to Nutrient Agar (NA) and NA+glucose. Plates 28 and 29 incubated at 30°C.
- Repeated rbs+dxs ligation (L11). (Yan, HX)
- Transformation of JM109 with L9 (pSB1A2+crtB )and L10 (pSB1A2+crtI-mut). Plates 30 to 33.
- possible rbs+dxs clones from Nimisha's plates were streaked to plate 34.
