Edinburgh/14 July 2008
From 2008.igem.org
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+ | table.calendar { margin: 0; padding: 2px; } | ||
+ | table.calendar td { margin: 0; padding: 1px; vertical-align: top; } | ||
+ | table.month .heading td { padding:1px; background-color: black; color: white; text-align:center; font-size:140%; font-weight:bold; } | ||
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- | * Digests and ligation '''L12''' to add rbs to ''appY'' clone M15 (sequence back today, seems OK). | + | </style> |
- | * Minipreps '''M31 to | + | </html> |
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+ | {|style="font color="#ffffff"; "background-color:"#cd0000"; cellpadding="0" cellspacing="4" border="4" bordercolor="#000"; border-spacing:0px; text-align:"center" width="250px" | ||
+ | </table> | ||
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+ | {| align="left" | ||
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+ | |align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=06}} | ||
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+ | |align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=07}} | ||
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+ | |align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=08}} | ||
+ | |} | ||
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+ | |||
+ | :::: '''[[Edinburgh/13_July_2008|< Previous Entry]]''' | ||
+ | |||
+ | == Week 5 == | ||
+ | === Monday 14 July 08 === | ||
+ | |||
+ | * Digests and ligation '''L12''' to add rbs to pSB1A2+''appY'' clone M15 (sequence back today, seems OK). | ||
+ | * Minipreps '''M31 to M36''' of ''crtB'' clones from plate 35 and '''M37 to to M42''' of ''crtI'' clones from plate 36. Digested with EcoRI/PstI. (AM) | ||
+ | * PCR '''P13''' and '''P14''' to mutated second EcoRI site on glgC clones M19 and M22. These were ran on '''Gel 13'''.<br /> | ||
+ | <br /> | ||
+ | |||
+ | :::: '''[[Edinburgh/15_July_2008|Next Entry >]]''' |
Revision as of 15:29, 21 August 2008
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Week 5
Monday 14 July 08
- Digests and ligation L12 to add rbs to pSB1A2+appY clone M15 (sequence back today, seems OK).
- Minipreps M31 to M36 of crtB clones from plate 35 and M37 to to M42 of crtI clones from plate 36. Digested with EcoRI/PstI. (AM)
- PCR P13 and P14 to mutated second EcoRI site on glgC clones M19 and M22. These were ran on Gel 13.