Edinburgh/28 July 2008

From 2008.igem.org

(Difference between revisions)
(New page: <html> <head> <title>Edinburgh iGEM 2008</title> <script type="text/javascript"> //Drop Down Tabs Menu- Author: Dynamic Drive (http://www.dynamicdrive.com) //Created: May 16th, 07' var ta...)
Line 289: Line 289:
</html>
</html>
-
=== Week 7 ===
+
<table>
-
==== Monday 28 July 08 ====
+
<html>
 +
<style type="text/css">
 +
table.calendar          { margin: 0; padding: 2px; }
 +
table.calendar td      { margin: 0; padding: 1px; vertical-align: top; }
 +
table.month .heading td { padding:1px; background-color: black; color: white; text-align:center; font-size:140%; font-weight:bold; }
 +
table.month .dow td    { color:#000000; text-align:center; font-size:100%; }
 +
table.month td.today    { background-color:#cd0000; }
 +
table.month td {
 +
    border: none;
 +
    margin: 0;
 +
    padding: 0pt 0.5pt;
 +
    font-weight: bold;
 +
    font-size: 9pt;
 +
    text-align: right;
 +
    background-color: white;
 +
    }
 +
#bodyContent table.month a { background:none; padding:0 }
 +
.day-active { color:#cd0000 }
 +
.day-empty  { color:#000000 }
-
* Repeated transformation of L20 (pSB1A2+rbs+''appY'') and L21 (pSB1A2+rbs+''crtB''), and plated on ''plates 63 to 66''. (YAN and Omar).
+
</style>
 +
</html>
 +
 
 +
 
 +
{|style="font color="#ffffff"; "background-color:"#cd0000"; cellpadding="0" cellspacing="4" border="4" bordercolor="#000"; border-spacing:0px; text-align:"center" width="250px"
 +
</table>
 +
 
 +
{| align="left"
 +
|-
 +
|align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=06}}
 +
|-
 +
|align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=07}}
 +
|-
 +
|align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=08}}
 +
|}
 +
 
 +
 
 +
:::: '''[[Edinburgh/27_July_2008|< Previous Entry]]'''
 +
 
 +
== Week 7 ==
 +
=== Monday 28 July 08 ===
 +
 
 +
* Repeated transformation of L20 (pSB1A2+rbs+''appY'') and L21 (pSB1A2+rbs+''crtB''), and plated on '''plates 63-66''. (Yan, Omar).
* PCR of ''cenA'', ''cenB'', ''cenC'' and ''cex'' from cell suspension ('''P38-41'''; cell solution incubated at 73-80°C for 8 minutes to kill cells/inactivate DNAse) and DNA solution 'purified' from contaminated cell culture ('''P42-45'''). Denaturing 95°C for 1 min, annealing 55°C for 10s, extension 70°C for 90s. PCR products run on '''Gel 26'''. (AM)
* PCR of ''cenA'', ''cenB'', ''cenC'' and ''cex'' from cell suspension ('''P38-41'''; cell solution incubated at 73-80°C for 8 minutes to kill cells/inactivate DNAse) and DNA solution 'purified' from contaminated cell culture ('''P42-45'''). Denaturing 95°C for 1 min, annealing 55°C for 10s, extension 70°C for 90s. PCR products run on '''Gel 26'''. (AM)
-
* Prepared '''L25''' of P36 (''glgC''-mut1,2 PCRed with primers f2 and r2) and Edinbrick 1. (AM)
+
* Prepared '''L25''' of P36 (''glgC''-mut1,2 PCRed with primers f2 and r2) and Edinbrick1. (AM)
-
* Minipreps '''M49 to M54''' of pSB1A2+rbs+''crtI'' transformants. (CF)
+
* Minipreps '''M49 to M54''' of possible pSB1A2+rbs+''crtI'' transformants. (CF)<br />
 +
<br />
 +
 
 +
:::: '''[[Edinburgh/29_July_2008|Next Entry >]]'''

Revision as of 14:58, 26 August 2008

Edinburgh iGEM 2008

 

June
MTWTFSS
            [http://2008.igem.org/wiki/index.php?title=Edinburgh/1_June_2008&action=edit 1]
[http://2008.igem.org/wiki/index.php?title=Edinburgh/2_June_2008&action=edit 2] [http://2008.igem.org/wiki/index.php?title=Edinburgh/3_June_2008&action=edit 3] [http://2008.igem.org/wiki/index.php?title=Edinburgh/4_June_2008&action=edit 4] [http://2008.igem.org/wiki/index.php?title=Edinburgh/5_June_2008&action=edit 5] [http://2008.igem.org/wiki/index.php?title=Edinburgh/6_June_2008&action=edit 6] [http://2008.igem.org/wiki/index.php?title=Edinburgh/7_June_2008&action=edit 7] [http://2008.igem.org/wiki/index.php?title=Edinburgh/8_June_2008&action=edit 8]
[http://2008.igem.org/wiki/index.php?title=Edinburgh/9_June_2008&action=edit 9] [http://2008.igem.org/wiki/index.php?title=Edinburgh/10_June_2008&action=edit 10] [http://2008.igem.org/wiki/index.php?title=Edinburgh/11_June_2008&action=edit 11] [http://2008.igem.org/wiki/index.php?title=Edinburgh/12_June_2008&action=edit 12] [http://2008.igem.org/wiki/index.php?title=Edinburgh/13_June_2008&action=edit 13] [http://2008.igem.org/wiki/index.php?title=Edinburgh/14_June_2008&action=edit 14] [http://2008.igem.org/wiki/index.php?title=Edinburgh/15_June_2008&action=edit 15]
[http://2008.igem.org/wiki/index.php?title=Edinburgh/16_June_2008&action=edit 16] [http://2008.igem.org/Edinburgh/17_June_2008 17] [http://2008.igem.org/wiki/index.php?title=Edinburgh/18_June_2008&action=edit 18] [http://2008.igem.org/Edinburgh/19_June_2008 19] [http://2008.igem.org/Edinburgh/20_June_2008 20] [http://2008.igem.org/wiki/index.php?title=Edinburgh/21_June_2008&action=edit 21] [http://2008.igem.org/wiki/index.php?title=Edinburgh/22_June_2008&action=edit 22]
[http://2008.igem.org/wiki/index.php?title=Edinburgh/23_June_2008&action=edit 23] [http://2008.igem.org/Edinburgh/24_June_2008 24] [http://2008.igem.org/Edinburgh/25_June_2008 25] [http://2008.igem.org/Edinburgh/26_June_2008 26] [http://2008.igem.org/Edinburgh/27_June_2008 27] [http://2008.igem.org/Edinburgh/28_June_2008 28] [http://2008.igem.org/Edinburgh/29_June_2008 29]
[http://2008.igem.org/Edinburgh/30_June_2008 30]
July
MTWTFSS
  [http://2008.igem.org/Edinburgh/1_July_2008 1] [http://2008.igem.org/Edinburgh/2_July_2008 2] [http://2008.igem.org/Edinburgh/3_July_2008 3] [http://2008.igem.org/Edinburgh/4_July_2008 4] [http://2008.igem.org/Edinburgh/5_July_2008 5] [http://2008.igem.org/Edinburgh/6_July_2008 6]
[http://2008.igem.org/Edinburgh/7_July_2008 7] [http://2008.igem.org/Edinburgh/8_July_2008 8] [http://2008.igem.org/Edinburgh/9_July_2008 9] [http://2008.igem.org/Edinburgh/10_July_2008 10] [http://2008.igem.org/Edinburgh/11_July_2008 11] [http://2008.igem.org/Edinburgh/12_July_2008 12] [http://2008.igem.org/Edinburgh/13_July_2008 13]
[http://2008.igem.org/Edinburgh/14_July_2008 14] [http://2008.igem.org/Edinburgh/15_July_2008 15] [http://2008.igem.org/Edinburgh/16_July_2008 16] [http://2008.igem.org/Edinburgh/17_July_2008 17] [http://2008.igem.org/Edinburgh/18_July_2008 18] [http://2008.igem.org/Edinburgh/19_July_2008 19] [http://2008.igem.org/Edinburgh/20_July_2008 20]
[http://2008.igem.org/Edinburgh/21_July_2008 21] [http://2008.igem.org/Edinburgh/22_July_2008 22] [http://2008.igem.org/Edinburgh/23_July_2008 23] [http://2008.igem.org/Edinburgh/24_July_2008 24] [http://2008.igem.org/Edinburgh/25_July_2008 25] [http://2008.igem.org/Edinburgh/26_July_2008 26] [http://2008.igem.org/Edinburgh/27_July_2008 27]
[http://2008.igem.org/Edinburgh/28_July_2008 28] [http://2008.igem.org/Edinburgh/29_July_2008 29] [http://2008.igem.org/Edinburgh/30_July_2008 30] [http://2008.igem.org/Edinburgh/31_July_2008 31]
August
MTWTFSS
        [http://2008.igem.org/Edinburgh/1_August_2008 1] [http://2008.igem.org/wiki/index.php?title=Edinburgh/2_August_2008&action=edit 2] [http://2008.igem.org/Edinburgh/3_August_2008 3]
[http://2008.igem.org/Edinburgh/4_August_2008 4] [http://2008.igem.org/Edinburgh/5_August_2008 5] [http://2008.igem.org/Edinburgh/6_August_2008 6] [http://2008.igem.org/Edinburgh/7_August_2008 7] [http://2008.igem.org/Edinburgh/8_August_2008 8] [http://2008.igem.org/Edinburgh/9_August_2008 9] [http://2008.igem.org/Edinburgh/10_August_2008 10]
[http://2008.igem.org/Edinburgh/11_August_2008 11] [http://2008.igem.org/Edinburgh/12_August_2008 12] [http://2008.igem.org/Edinburgh/13_August_2008 13] [http://2008.igem.org/Edinburgh/14_August_2008 14] [http://2008.igem.org/Edinburgh/15_August_2008 15] [http://2008.igem.org/Edinburgh/16_August_2008 16] [http://2008.igem.org/Edinburgh/17_August_2008 17]
[http://2008.igem.org/Edinburgh/18_August_2008 18] [http://2008.igem.org/Edinburgh/19_August_2008 19] [http://2008.igem.org/Edinburgh/20_August_2008 20] [http://2008.igem.org/Edinburgh/21_August_2008 21] [http://2008.igem.org/Edinburgh/22_August_2008 22] [http://2008.igem.org/wiki/index.php?title=Edinburgh/23_August_2008&action=edit 23] [http://2008.igem.org/wiki/index.php?title=Edinburgh/24_August_2008&action=edit 24]
[http://2008.igem.org/Edinburgh/25_August_2008 25] [http://2008.igem.org/Edinburgh/26_August_2008 26] [http://2008.igem.org/Edinburgh/27_August_2008 27] [http://2008.igem.org/Edinburgh/28_August_2008 28] [http://2008.igem.org/Edinburgh/29_August_2008 29] [http://2008.igem.org/wiki/index.php?title=Edinburgh/30_August_2008&action=edit 30] [http://2008.igem.org/wiki/index.php?title=Edinburgh/31_August_2008&action=edit 31]


< Previous Entry

Week 7

Monday 28 July 08

  • Repeated transformation of L20 (pSB1A2+rbs+appY) and L21 (pSB1A2+rbs+crtB), and plated on 'plates 63-66. (Yan, Omar).
  • PCR of cenA, cenB, cenC and cex from cell suspension (P38-41; cell solution incubated at 73-80°C for 8 minutes to kill cells/inactivate DNAse) and DNA solution 'purified' from contaminated cell culture (P42-45). Denaturing 95°C for 1 min, annealing 55°C for 10s, extension 70°C for 90s. PCR products run on Gel 26. (AM)
  • Prepared L25 of P36 (glgC-mut1,2 PCRed with primers f2 and r2) and Edinbrick1. (AM)
  • Minipreps M49 to M54 of possible pSB1A2+rbs+crtI transformants. (CF)


Next Entry >
Retrieved from "http://2008.igem.org/Edinburgh/28_July_2008"