Edinburgh/27 June 2008

From 2008.igem.org

(Difference between revisions)
(→Friday 27 June 08)
(→Friday 27 June 08)
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* Ordered ''Cellulomonas fimi'' ATCC 484 (NCIB 8980, DSM 20113) from DSMZ.
* Ordered ''Cellulomonas fimi'' ATCC 484 (NCIB 8980, DSM 20113) from DSMZ.
* Transformations of the iGEM competent JM109 cells with ligations L1 to L4. In each case, 100μl of cells were transformed with 5μl of ligation, and the remaining 5μl of each ligation was frozen so that it could be analysed if the transformations fail. Fresh Blue-White ampicillin plates were prepared.
* Transformations of the iGEM competent JM109 cells with ligations L1 to L4. In each case, 100μl of cells were transformed with 5μl of ligation, and the remaining 5μl of each ligation was frozen so that it could be analysed if the transformations fail. Fresh Blue-White ampicillin plates were prepared.
-
** '''Plate 3''': Ligation L1, 100μl
+
** '''Plate 3''': L1 (pSB1A2+''dxs'') transformation, 100μl
-
** '''Plate 4''': Ligation L2, 100μl
+
** '''Plate 4''': L2 (pSB1A2+''appY'') transformation, 100μl
-
** '''Plate 5''': Ligation L3, 100μl
+
** '''Plate 5''': L3 (BABEL1+''glgC'') transformation, 100μl
-
** '''Plate 6''': Ligation L4, 100μl
+
** '''Plate 6''': L4 (BABEL2+''glgC'') transformation, 100μl
-
** '''Plate 7''': Ligation L1, 900μl
+
** '''Plate 7''': L1 (pSB1A2+''dxs'') transformation, 900μl
-
** '''Plate 8''': Ligation L2, 900μl
+
** '''Plate 8''': L2 (pSB1A2+''appY'') transformation, 900μl
-
** '''Plate 9''': Ligation L3, 900μl
+
** '''Plate 9''': L3 (BABEL1+''glgC'') transformation, 900μl
-
** '''Plate 10''': Ligation L4, 900μl
+
** '''Plate 10''': L4 (BABEL2+''glgC'') transformation, 900μl

Revision as of 14:17, 20 August 2008

Edinburgh iGEM 2008

 

Week 2

Friday 27 June 08

  • Ordered Cellulomonas fimi ATCC 484 (NCIB 8980, DSM 20113) from DSMZ.
  • Transformations of the iGEM competent JM109 cells with ligations L1 to L4. In each case, 100μl of cells were transformed with 5μl of ligation, and the remaining 5μl of each ligation was frozen so that it could be analysed if the transformations fail. Fresh Blue-White ampicillin plates were prepared.
    • Plate 3: L1 (pSB1A2+dxs) transformation, 100μl
    • Plate 4: L2 (pSB1A2+appY) transformation, 100μl
    • Plate 5: L3 (BABEL1+glgC) transformation, 100μl
    • Plate 6: L4 (BABEL2+glgC) transformation, 100μl
    • Plate 7: L1 (pSB1A2+dxs) transformation, 900μl
    • Plate 8: L2 (pSB1A2+appY) transformation, 900μl
    • Plate 9: L3 (BABEL1+glgC) transformation, 900μl
    • Plate 10: L4 (BABEL2+glgC) transformation, 900μl
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