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Edinburgh/27 June 2008
From 2008.igem.org
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Week 2
Friday 27 June 08
- Ordered Cellulomonas fimi ATCC 484 (NCIB 8980, DSM 20113) from DSMZ.
- Transformations of the iGEM competent JM109 cells with ligations L1 to L4. In each case, 100μl of cells were transformed with 5μl of ligation, and the remaining 5μl of each ligation was frozen so that it could be analysed if the transformations fail. Fresh Blue-White ampicillin plates were prepared.
- Plate 3: L1 (pSB1A2+dxs) transformation, 100μl
- Plate 4: L2 (pSB1A2+appY) transformation, 100μl
- Plate 5: L3 (BABEL1+glgC) transformation, 100μl
- Plate 6: L4 (BABEL2+glgC) transformation, 100μl
- Plate 7: L1 (pSB1A2+dxs) transformation, 900μl
- Plate 8: L2 (pSB1A2+appY) transformation, 900μl
- Plate 9: L3 (BABEL1+glgC) transformation, 900μl
- Plate 10: L4 (BABEL2+glgC) transformation, 900μl
