Edinburgh/9 July 2008
From 2008.igem.org
(Difference between revisions)
(→Wednesday 9 July 08) |
|||
Line 271: | Line 271: | ||
<li><a href="https://2008.igem.org/Team:Edinburgh" title="Home"><span>Home</span></a></li> | <li><a href="https://2008.igem.org/Team:Edinburgh" title="Home"><span>Home</span></a></li> | ||
<li><a href="https://2008.igem.org/Team:Edinburgh/Project" title="Project" rel="dropmenu1_a"><span>The Project</span></a></li> | <li><a href="https://2008.igem.org/Team:Edinburgh/Project" title="Project" rel="dropmenu1_a"><span>The Project</span></a></li> | ||
- | |||
- | |||
<li><a href="https://2008.igem.org/Team:Edinburgh/Modeling" title="Modelling" rel="dropmenu2_a"><span>Modelling</span></a></li> | <li><a href="https://2008.igem.org/Team:Edinburgh/Modeling" title="Modelling" rel="dropmenu2_a"><span>Modelling</span></a></li> | ||
- | <li><a href="https://2008.igem.org/Team:Edinburgh/Notebook" title="Notebook"><span>Notebook</span></a></li> | + | <li><a href="https://2008.igem.org/Team:Edinburgh/Notebook" title="Notebook"><span>Notebook</span></a></li> |
+ | <li><a href="https://2008.igem.org/Team:Edinburgh/Results" title="Results" rel="dropmenu1_a"><span>Results</span></a></li> | ||
+ | <li><a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=Edinburgh" title="Parts" rel="dropmenu1_a"><span>BioBrick Parts</span></a></li> | ||
+ | <li><a href="https://2008.igem.org/Team:Edinburgh/Team" title="Team" ><span>The Team</span></a></li> | ||
</ul> | </ul> | ||
</div> | </div> | ||
Line 325: | Line 326: | ||
|align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=08}} | |align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=08}} | ||
|} | |} | ||
- | |||
:::: '''[[Edinburgh/8_July_2008|< Previous Entry]]''' | :::: '''[[Edinburgh/8_July_2008|< Previous Entry]]''' |
Revision as of 09:56, 28 August 2008
| ||||||||||||||||||||||||||||||||||||||||||||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||
|
Week 4
Wednesday 9 July 08
- Results of L6 (BABEL2+glgC-mut1) and L7 (BABEL2+glgC-mut2) transformations: no colonies for L6, but 9 colonies were obtained from L7. These were subcultured to plates 26 and 27. By the end of the day, these had grown sufficiently for 6 overnight cultures to be set up for minipreps tomorrow. In theory, some or all of these should be glgC with EcoRI site 2 mutated out.
- Minipreps M13 to M18 of possible pSB1A2+appY transformants. Digests with EcoRI/PstI were run on Gel 10. All but M13 looked OK (insert band on M14 was so faint as to be ambiguous, but M15 to M18 all look good).
- Digests and ligation L8 for addition of a synthetic ribosome binding site M2 (which has now been entered into the Registry as BBa_K118000).